Influence of Coating and Size of Magnetic Nanoparticles on Cellular Uptake for In Vitro MRI

Iron oxide nanoparticles (IONPs) are suitable materials for contrast enhancement in magnetic resonance imaging (MRI). Their potential clinical applications range from diagnosis to therapy and follow-up treatments. However, a deeper understanding of the interaction between IONPs, culture media and ce...

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Autores principales:	Belén Cortés-Llanos, Sandra M. Ocampo, Leonor de la Cueva, Gabriel F. Calvo, Juan Belmonte-Beitia, Lucas Pérez, Gorka Salas, Ángel Ayuso-Sacido
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	iron oxide nanoparticles colloidal properties cellular uptake magnetic resonance imaging Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/8a730299440040728ac75fa5e9cc84d6
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spelling	oai:doaj.org-article:8a730299440040728ac75fa5e9cc84d62021-11-25T18:30:36ZInfluence of Coating and Size of Magnetic Nanoparticles on Cellular Uptake for In Vitro MRI10.3390/nano111128882079-4991https://doaj.org/article/8a730299440040728ac75fa5e9cc84d62021-10-01T00:00:00Zhttps://www.mdpi.com/2079-4991/11/11/2888https://doaj.org/toc/2079-4991Iron oxide nanoparticles (IONPs) are suitable materials for contrast enhancement in magnetic resonance imaging (MRI). Their potential clinical applications range from diagnosis to therapy and follow-up treatments. However, a deeper understanding of the interaction between IONPs, culture media and cells is necessary for expanding the application of this technology to different types of cancer therapies. To achieve new insights of these interactions, a set of IONPs were prepared with the same inorganic core and five distinct coatings, to study their aggregation and interactions in different physiological media, as well as their cell labelling efficiency. Then, a second set of IONPs, with six different core sizes and the same coating, were used to study how the core size affects cell labelling and MRI in vitro. Here, IONPs suspended in biological media experience a partial removal of the coating and adhesion of molecules. The FBS concentration alters the labelling of all types of IONPs and hydrodynamic sizes ≥ 300 nm provide the greatest labelling using the centrifugation-mediated internalization (CMI). The best contrast for MRI results requires a core size range between 12–14 nm coated with dimercaptosuccinic acid (DMSA) producing <i>R</i><sub>2</sub><sup>*</sup> values of 393.7 s<sup>−1</sup> and 428.3 s<sup>−1</sup>, respectively. These findings will help to bring IONPs as negative contrast agents into clinical settings.Belén Cortés-LlanosSandra M. OcampoLeonor de la CuevaGabriel F. CalvoJuan Belmonte-BeitiaLucas PérezGorka SalasÁngel Ayuso-SacidoMDPI AGarticleiron oxide nanoparticlescolloidal propertiescellular uptakemagnetic resonance imagingChemistryQD1-999ENNanomaterials, Vol 11, Iss 2888, p 2888 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	iron oxide nanoparticles colloidal properties cellular uptake magnetic resonance imaging Chemistry QD1-999
spellingShingle	iron oxide nanoparticles colloidal properties cellular uptake magnetic resonance imaging Chemistry QD1-999 Belén Cortés-Llanos Sandra M. Ocampo Leonor de la Cueva Gabriel F. Calvo Juan Belmonte-Beitia Lucas Pérez Gorka Salas Ángel Ayuso-Sacido Influence of Coating and Size of Magnetic Nanoparticles on Cellular Uptake for In Vitro MRI
description	Iron oxide nanoparticles (IONPs) are suitable materials for contrast enhancement in magnetic resonance imaging (MRI). Their potential clinical applications range from diagnosis to therapy and follow-up treatments. However, a deeper understanding of the interaction between IONPs, culture media and cells is necessary for expanding the application of this technology to different types of cancer therapies. To achieve new insights of these interactions, a set of IONPs were prepared with the same inorganic core and five distinct coatings, to study their aggregation and interactions in different physiological media, as well as their cell labelling efficiency. Then, a second set of IONPs, with six different core sizes and the same coating, were used to study how the core size affects cell labelling and MRI in vitro. Here, IONPs suspended in biological media experience a partial removal of the coating and adhesion of molecules. The FBS concentration alters the labelling of all types of IONPs and hydrodynamic sizes ≥ 300 nm provide the greatest labelling using the centrifugation-mediated internalization (CMI). The best contrast for MRI results requires a core size range between 12–14 nm coated with dimercaptosuccinic acid (DMSA) producing <i>R</i><sub>2</sub><sup>*</sup> values of 393.7 s<sup>−1</sup> and 428.3 s<sup>−1</sup>, respectively. These findings will help to bring IONPs as negative contrast agents into clinical settings.
format	article
author	Belén Cortés-Llanos Sandra M. Ocampo Leonor de la Cueva Gabriel F. Calvo Juan Belmonte-Beitia Lucas Pérez Gorka Salas Ángel Ayuso-Sacido
author_facet	Belén Cortés-Llanos Sandra M. Ocampo Leonor de la Cueva Gabriel F. Calvo Juan Belmonte-Beitia Lucas Pérez Gorka Salas Ángel Ayuso-Sacido
author_sort	Belén Cortés-Llanos
title	Influence of Coating and Size of Magnetic Nanoparticles on Cellular Uptake for In Vitro MRI
title_short	Influence of Coating and Size of Magnetic Nanoparticles on Cellular Uptake for In Vitro MRI
title_full	Influence of Coating and Size of Magnetic Nanoparticles on Cellular Uptake for In Vitro MRI
title_fullStr	Influence of Coating and Size of Magnetic Nanoparticles on Cellular Uptake for In Vitro MRI
title_full_unstemmed	Influence of Coating and Size of Magnetic Nanoparticles on Cellular Uptake for In Vitro MRI
title_sort	influence of coating and size of magnetic nanoparticles on cellular uptake for in vitro mri
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8a730299440040728ac75fa5e9cc84d6
work_keys_str_mv	AT belencortesllanos influenceofcoatingandsizeofmagneticnanoparticlesoncellularuptakeforinvitromri AT sandramocampo influenceofcoatingandsizeofmagneticnanoparticlesoncellularuptakeforinvitromri AT leonordelacueva influenceofcoatingandsizeofmagneticnanoparticlesoncellularuptakeforinvitromri AT gabrielfcalvo influenceofcoatingandsizeofmagneticnanoparticlesoncellularuptakeforinvitromri AT juanbelmontebeitia influenceofcoatingandsizeofmagneticnanoparticlesoncellularuptakeforinvitromri AT lucasperez influenceofcoatingandsizeofmagneticnanoparticlesoncellularuptakeforinvitromri AT gorkasalas influenceofcoatingandsizeofmagneticnanoparticlesoncellularuptakeforinvitromri AT angelayusosacido influenceofcoatingandsizeofmagneticnanoparticlesoncellularuptakeforinvitromri
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Influence of Coating and Size of Magnetic Nanoparticles on Cellular Uptake for In Vitro MRI

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