MODERN TECHNOLOGIES AND APPROACHES TO APOPTOSIS STUDIES IN EXPERIMENTAL BIOLOGY

Abstract. This review is focused on analysis of currently used flow cytometric methods designed foridentifying apoptotic cells in various invertebrate and vertebrate species. Apoptosis can be characterized by stage-specific morphological and biochemical changes that are typical to all kinds of eukar...

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Autores principales:	I. V. Kudriavtsev, A. S. Golovkin, A. V. Zurochka, S. V. Khaidukov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	apoptosis criteria evaluation techniques interspecies comparisons. Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/8a76cf6f106943b4b7b254f5afd3f53e
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spelling	oai:doaj.org-article:8a76cf6f106943b4b7b254f5afd3f53e2021-11-18T08:03:42ZMODERN TECHNOLOGIES AND APPROACHES TO APOPTOSIS STUDIES IN EXPERIMENTAL BIOLOGY1563-06252313-741X10.15789/1563-0625-2012-6-461-482https://doaj.org/article/8a76cf6f106943b4b7b254f5afd3f53e2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/534https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. This review is focused on analysis of currently used flow cytometric methods designed foridentifying apoptotic cells in various invertebrate and vertebrate species. Apoptosis can be characterized by stage-specific morphological and biochemical changes that are typical to all kinds of eukaryotic cells. In this article, we consider different techniques of apoptosis detection based on assessment of cellular morphology and plasma membrane alterations, activation of intracellular enzymes and components of a caspase cascade, as well as DNA fragmentation and failure of mitochondrial transmembrane potential, as assessed in various animal groups. Apoptosis recognized as a key mechanism aiming at maintenance of cellular homeostasis in multicellular organisms, and such investigations represent a necessary component of fundamental and applied studies in diverse fields of experimental biology and immunology. A broad spectrum of apoptosis markers isused, and the preference is given to optimal approaches, as determined by experimental tasks, and technical opportunities of the laboratory.I. V. KudriavtsevA. S. GolovkinA. V. ZurochkaS. V. KhaidukovSPb RAACIarticleapoptosiscriteriaevaluation techniquesinterspecies comparisons.Immunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 14, Iss 6, Pp 461-482 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	apoptosis criteria evaluation techniques interspecies comparisons. Immunologic diseases. Allergy RC581-607
spellingShingle	apoptosis criteria evaluation techniques interspecies comparisons. Immunologic diseases. Allergy RC581-607 I. V. Kudriavtsev A. S. Golovkin A. V. Zurochka S. V. Khaidukov MODERN TECHNOLOGIES AND APPROACHES TO APOPTOSIS STUDIES IN EXPERIMENTAL BIOLOGY
description	Abstract. This review is focused on analysis of currently used flow cytometric methods designed foridentifying apoptotic cells in various invertebrate and vertebrate species. Apoptosis can be characterized by stage-specific morphological and biochemical changes that are typical to all kinds of eukaryotic cells. In this article, we consider different techniques of apoptosis detection based on assessment of cellular morphology and plasma membrane alterations, activation of intracellular enzymes and components of a caspase cascade, as well as DNA fragmentation and failure of mitochondrial transmembrane potential, as assessed in various animal groups. Apoptosis recognized as a key mechanism aiming at maintenance of cellular homeostasis in multicellular organisms, and such investigations represent a necessary component of fundamental and applied studies in diverse fields of experimental biology and immunology. A broad spectrum of apoptosis markers isused, and the preference is given to optimal approaches, as determined by experimental tasks, and technical opportunities of the laboratory.
format	article
author	I. V. Kudriavtsev A. S. Golovkin A. V. Zurochka S. V. Khaidukov
author_facet	I. V. Kudriavtsev A. S. Golovkin A. V. Zurochka S. V. Khaidukov
author_sort	I. V. Kudriavtsev
title	MODERN TECHNOLOGIES AND APPROACHES TO APOPTOSIS STUDIES IN EXPERIMENTAL BIOLOGY
title_short	MODERN TECHNOLOGIES AND APPROACHES TO APOPTOSIS STUDIES IN EXPERIMENTAL BIOLOGY
title_full	MODERN TECHNOLOGIES AND APPROACHES TO APOPTOSIS STUDIES IN EXPERIMENTAL BIOLOGY
title_fullStr	MODERN TECHNOLOGIES AND APPROACHES TO APOPTOSIS STUDIES IN EXPERIMENTAL BIOLOGY
title_full_unstemmed	MODERN TECHNOLOGIES AND APPROACHES TO APOPTOSIS STUDIES IN EXPERIMENTAL BIOLOGY
title_sort	modern technologies and approaches to apoptosis studies in experimental biology
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/8a76cf6f106943b4b7b254f5afd3f53e
work_keys_str_mv	AT ivkudriavtsev moderntechnologiesandapproachestoapoptosisstudiesinexperimentalbiology AT asgolovkin moderntechnologiesandapproachestoapoptosisstudiesinexperimentalbiology AT avzurochka moderntechnologiesandapproachestoapoptosisstudiesinexperimentalbiology AT svkhaidukov moderntechnologiesandapproachestoapoptosisstudiesinexperimentalbiology
_version_	1718422490298974208

MODERN TECHNOLOGIES AND APPROACHES TO APOPTOSIS STUDIES IN EXPERIMENTAL BIOLOGY

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