Inherent interfacial mechanical gradients in 3D hydrogels influence tumor cell behaviors.

Cells sense and respond to the rigidity of their microenvironment by altering their morphology and migration behavior. To examine this response, hydrogels with a range of moduli or mechanical gradients have been developed. Here, we show that edge effects inherent in hydrogels supported on rigid subs...

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Autores principales:	Shreyas S Rao, Sarah Bentil, Jessica DeJesus, John Larison, Alex Hissong, Rebecca Dupaix, Atom Sarkar, Jessica O Winter
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8a7b3c4efca849ed88427e8140783a73
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spelling	oai:doaj.org-article:8a7b3c4efca849ed88427e8140783a732021-11-18T07:20:54ZInherent interfacial mechanical gradients in 3D hydrogels influence tumor cell behaviors.1932-620310.1371/journal.pone.0035852https://doaj.org/article/8a7b3c4efca849ed88427e8140783a732012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22558241/?tool=EBIhttps://doaj.org/toc/1932-6203Cells sense and respond to the rigidity of their microenvironment by altering their morphology and migration behavior. To examine this response, hydrogels with a range of moduli or mechanical gradients have been developed. Here, we show that edge effects inherent in hydrogels supported on rigid substrates also influence cell behavior. A Matrigel hydrogel was supported on a rigid glass substrate, an interface which computational techniques revealed to yield relative stiffening close to the rigid substrate support. To explore the influence of these gradients in 3D, hydrogels of varying Matrigel content were synthesized and the morphology, spreading, actin organization, and migration of glioblastoma multiforme (GBM) tumor cells were examined at the lowest (<50 µm) and highest (>500 µm) gel positions. GBMs adopted bipolar morphologies, displayed actin stress fiber formation, and evidenced fast, mesenchymal migration close to the substrate, whereas away from the interface, they adopted more rounded or ellipsoid morphologies, displayed poor actin architecture, and evidenced slow migration with some amoeboid characteristics. Mechanical gradients produced via edge effects could be observed with other hydrogels and substrates and permit observation of responses to multiple mechanical environments in a single hydrogel. Thus, hydrogel-support edge effects could be used to explore mechanosensitivity in a single 3D hydrogel system and should be considered in 3D hydrogel cell culture systems.Shreyas S RaoSarah BentilJessica DeJesusJohn LarisonAlex HissongRebecca DupaixAtom SarkarJessica O WinterPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e35852 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shreyas S Rao Sarah Bentil Jessica DeJesus John Larison Alex Hissong Rebecca Dupaix Atom Sarkar Jessica O Winter Inherent interfacial mechanical gradients in 3D hydrogels influence tumor cell behaviors.
description	Cells sense and respond to the rigidity of their microenvironment by altering their morphology and migration behavior. To examine this response, hydrogels with a range of moduli or mechanical gradients have been developed. Here, we show that edge effects inherent in hydrogels supported on rigid substrates also influence cell behavior. A Matrigel hydrogel was supported on a rigid glass substrate, an interface which computational techniques revealed to yield relative stiffening close to the rigid substrate support. To explore the influence of these gradients in 3D, hydrogels of varying Matrigel content were synthesized and the morphology, spreading, actin organization, and migration of glioblastoma multiforme (GBM) tumor cells were examined at the lowest (<50 µm) and highest (>500 µm) gel positions. GBMs adopted bipolar morphologies, displayed actin stress fiber formation, and evidenced fast, mesenchymal migration close to the substrate, whereas away from the interface, they adopted more rounded or ellipsoid morphologies, displayed poor actin architecture, and evidenced slow migration with some amoeboid characteristics. Mechanical gradients produced via edge effects could be observed with other hydrogels and substrates and permit observation of responses to multiple mechanical environments in a single hydrogel. Thus, hydrogel-support edge effects could be used to explore mechanosensitivity in a single 3D hydrogel system and should be considered in 3D hydrogel cell culture systems.
format	article
author	Shreyas S Rao Sarah Bentil Jessica DeJesus John Larison Alex Hissong Rebecca Dupaix Atom Sarkar Jessica O Winter
author_facet	Shreyas S Rao Sarah Bentil Jessica DeJesus John Larison Alex Hissong Rebecca Dupaix Atom Sarkar Jessica O Winter
author_sort	Shreyas S Rao
title	Inherent interfacial mechanical gradients in 3D hydrogels influence tumor cell behaviors.
title_short	Inherent interfacial mechanical gradients in 3D hydrogels influence tumor cell behaviors.
title_full	Inherent interfacial mechanical gradients in 3D hydrogels influence tumor cell behaviors.
title_fullStr	Inherent interfacial mechanical gradients in 3D hydrogels influence tumor cell behaviors.
title_full_unstemmed	Inherent interfacial mechanical gradients in 3D hydrogels influence tumor cell behaviors.
title_sort	inherent interfacial mechanical gradients in 3d hydrogels influence tumor cell behaviors.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/8a7b3c4efca849ed88427e8140783a73
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Inherent interfacial mechanical gradients in 3D hydrogels influence tumor cell behaviors.

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