The Antibacterial Synthetic Flavonoid BrCl-Flav Exhibits Important Anti-Candida Activity by Damaging Cell Membrane Integrity

Infections caused by Candida are very difficult to treat due to increasing antifungal resistance. Recent studies showed that patients with Candida infections resistant to fluconazole have very few treatment options. Therefore, finding new efficient antifungal ag...

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Autores principales:	Cornelia Babii, Mihaela Savu, Iuliana Motrescu, Lucian Mihail Birsa, Laura Gabriela Sarbu, Marius Stefan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	anti-<i>Candida</i> synthetic flavonoid antifungal agents mechanism of action synergistic effect Medicine R Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/8a8925e98d8d423eaba9dfc7543925c5
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spelling	oai:doaj.org-article:8a8925e98d8d423eaba9dfc7543925c52021-11-25T18:39:35ZThe Antibacterial Synthetic Flavonoid BrCl-Flav Exhibits Important Anti-<i>Candida</i> Activity by Damaging Cell Membrane Integrity10.3390/ph141111301424-8247https://doaj.org/article/8a8925e98d8d423eaba9dfc7543925c52021-11-01T00:00:00Zhttps://www.mdpi.com/1424-8247/14/11/1130https://doaj.org/toc/1424-8247Infections caused by <i>Candida</i> are very difficult to treat due to increasing antifungal resistance. Recent studies showed that patients with <i>Candida</i> infections resistant to fluconazole have very few treatment options. Therefore, finding new efficient antifungal agents is a matter of medical high priority. The aim of this study was to explore the antifungal potential of BrCl-flav-a representative of a new class of synthetic flavonoids with bromine as halogen substituent at the benzopyran core against four <i>Candida</i> clinical strains. Determination of minimum inhibitory concentration and minimum fungicidal concentration along with the time kill assay indicated a strong antifungal effect of BrCl-flav against <i>C. albicans</i>, <i>C. parapsilosis</i>, <i>C. krusei</i> and <i>C. glabrata</i>. The investigation of anti-<i>Candida</i> mechanism of action using fluorescence microscopy and scanning electron microscopy revealed that Br-Cl flav could inhibit fungal growth by impairing the membrane integrity, the resulting structural damages leading to cell lysis. BrCl-flav also showed important anti-virulence properties against <i>Candida</i> spp., inhibiting biofilm formation and yeast to hyphal transition. A strong synergistic antifungal effect against <i>C. albicans</i> strain was observed when BrCl-flav was used in combination with fluconazole. BrCl-flav has a good potential to develop new effective antifungal agents in the context of <i>Candida</i> spp. multidrug resistance phenomenon.Cornelia BabiiMihaela SavuIuliana MotrescuLucian Mihail BirsaLaura Gabriela SarbuMarius StefanMDPI AGarticleanti-<i>Candida</i>synthetic flavonoidantifungal agentsmechanism of actionsynergistic effectMedicineRPharmacy and materia medicaRS1-441ENPharmaceuticals, Vol 14, Iss 1130, p 1130 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	anti-<i>Candida</i> synthetic flavonoid antifungal agents mechanism of action synergistic effect Medicine R Pharmacy and materia medica RS1-441
spellingShingle	anti-<i>Candida</i> synthetic flavonoid antifungal agents mechanism of action synergistic effect Medicine R Pharmacy and materia medica RS1-441 Cornelia Babii Mihaela Savu Iuliana Motrescu Lucian Mihail Birsa Laura Gabriela Sarbu Marius Stefan The Antibacterial Synthetic Flavonoid BrCl-Flav Exhibits Important Anti-<i>Candida</i> Activity by Damaging Cell Membrane Integrity
description	Infections caused by <i>Candida</i> are very difficult to treat due to increasing antifungal resistance. Recent studies showed that patients with <i>Candida</i> infections resistant to fluconazole have very few treatment options. Therefore, finding new efficient antifungal agents is a matter of medical high priority. The aim of this study was to explore the antifungal potential of BrCl-flav-a representative of a new class of synthetic flavonoids with bromine as halogen substituent at the benzopyran core against four <i>Candida</i> clinical strains. Determination of minimum inhibitory concentration and minimum fungicidal concentration along with the time kill assay indicated a strong antifungal effect of BrCl-flav against <i>C. albicans</i>, <i>C. parapsilosis</i>, <i>C. krusei</i> and <i>C. glabrata</i>. The investigation of anti-<i>Candida</i> mechanism of action using fluorescence microscopy and scanning electron microscopy revealed that Br-Cl flav could inhibit fungal growth by impairing the membrane integrity, the resulting structural damages leading to cell lysis. BrCl-flav also showed important anti-virulence properties against <i>Candida</i> spp., inhibiting biofilm formation and yeast to hyphal transition. A strong synergistic antifungal effect against <i>C. albicans</i> strain was observed when BrCl-flav was used in combination with fluconazole. BrCl-flav has a good potential to develop new effective antifungal agents in the context of <i>Candida</i> spp. multidrug resistance phenomenon.
format	article
author	Cornelia Babii Mihaela Savu Iuliana Motrescu Lucian Mihail Birsa Laura Gabriela Sarbu Marius Stefan
author_facet	Cornelia Babii Mihaela Savu Iuliana Motrescu Lucian Mihail Birsa Laura Gabriela Sarbu Marius Stefan
author_sort	Cornelia Babii
title	The Antibacterial Synthetic Flavonoid BrCl-Flav Exhibits Important Anti-<i>Candida</i> Activity by Damaging Cell Membrane Integrity
title_short	The Antibacterial Synthetic Flavonoid BrCl-Flav Exhibits Important Anti-<i>Candida</i> Activity by Damaging Cell Membrane Integrity
title_full	The Antibacterial Synthetic Flavonoid BrCl-Flav Exhibits Important Anti-<i>Candida</i> Activity by Damaging Cell Membrane Integrity
title_fullStr	The Antibacterial Synthetic Flavonoid BrCl-Flav Exhibits Important Anti-<i>Candida</i> Activity by Damaging Cell Membrane Integrity
title_full_unstemmed	The Antibacterial Synthetic Flavonoid BrCl-Flav Exhibits Important Anti-<i>Candida</i> Activity by Damaging Cell Membrane Integrity
title_sort	antibacterial synthetic flavonoid brcl-flav exhibits important anti-<i>candida</i> activity by damaging cell membrane integrity
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8a8925e98d8d423eaba9dfc7543925c5
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The Antibacterial Synthetic Flavonoid BrCl-Flav Exhibits Important Anti-<i>Candida</i> Activity by Damaging Cell Membrane Integrity

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