Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution

It remains a challenge to obtain high-resolution structures of molecules smaller than 200 kDa using single particle cryo-EM. Here, the authors apply the Cs-corrector-VPP coupled cryo-EM and solve structures of the 52 kDa streptavidin (SA) protein at near-atomic resolution.

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Autores principales:	Xiao Fan, Jia Wang, Xing Zhang, Zi Yang, Jin-Can Zhang, Lingyun Zhao, Hai-Lin Peng, Jianlin Lei, Hong-Wei Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/8a89421a2bf6405aa235a43e887d674f
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spelling	oai:doaj.org-article:8a89421a2bf6405aa235a43e887d674f2021-12-02T17:31:37ZSingle particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution10.1038/s41467-019-10368-w2041-1723https://doaj.org/article/8a89421a2bf6405aa235a43e887d674f2019-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-10368-whttps://doaj.org/toc/2041-1723It remains a challenge to obtain high-resolution structures of molecules smaller than 200 kDa using single particle cryo-EM. Here, the authors apply the Cs-corrector-VPP coupled cryo-EM and solve structures of the 52 kDa streptavidin (SA) protein at near-atomic resolution.Xiao FanJia WangXing ZhangZi YangJin-Can ZhangLingyun ZhaoHai-Lin PengJianlin LeiHong-Wei WangNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Xiao Fan Jia Wang Xing Zhang Zi Yang Jin-Can Zhang Lingyun Zhao Hai-Lin Peng Jianlin Lei Hong-Wei Wang Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution
description	It remains a challenge to obtain high-resolution structures of molecules smaller than 200 kDa using single particle cryo-EM. Here, the authors apply the Cs-corrector-VPP coupled cryo-EM and solve structures of the 52 kDa streptavidin (SA) protein at near-atomic resolution.
format	article
author	Xiao Fan Jia Wang Xing Zhang Zi Yang Jin-Can Zhang Lingyun Zhao Hai-Lin Peng Jianlin Lei Hong-Wei Wang
author_facet	Xiao Fan Jia Wang Xing Zhang Zi Yang Jin-Can Zhang Lingyun Zhao Hai-Lin Peng Jianlin Lei Hong-Wei Wang
author_sort	Xiao Fan
title	Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution
title_short	Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution
title_full	Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution
title_fullStr	Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution
title_full_unstemmed	Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution
title_sort	single particle cryo-em reconstruction of 52 kda streptavidin at 3.2 angstrom resolution
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/8a89421a2bf6405aa235a43e887d674f
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Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution

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