Transgenic biofortification of the starchy staple cassava (Manihot esculenta) generates a novel sink for protein.

Although calorie dense, the starchy, tuberous roots of cassava provide the lowest sources of dietary protein within the major staple food crops (Manihot esculenta Crantz). (Montagnac JA, Davis CR, Tanumihardjo SA. (2009) Compr Rev Food Sci Food Saf 8:181-194). Cassava was genetically modified to exp...

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Autores principales: Mohammad Abhary, Dimuth Siritunga, Gene Stevens, Nigel J Taylor, Claude M Fauquet
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Publicado: Public Library of Science (PLoS) 2011
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Acceso en línea:https://doaj.org/article/8a8bdec754df4eef9ce6043416993583
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spelling oai:doaj.org-article:8a8bdec754df4eef9ce60434169935832021-11-18T06:59:54ZTransgenic biofortification of the starchy staple cassava (Manihot esculenta) generates a novel sink for protein.1932-620310.1371/journal.pone.0016256https://doaj.org/article/8a8bdec754df4eef9ce60434169935832011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21283593/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Although calorie dense, the starchy, tuberous roots of cassava provide the lowest sources of dietary protein within the major staple food crops (Manihot esculenta Crantz). (Montagnac JA, Davis CR, Tanumihardjo SA. (2009) Compr Rev Food Sci Food Saf 8:181-194). Cassava was genetically modified to express zeolin, a nutritionally balanced storage protein under control of the patatin promoter. Transgenic plants accumulated zeolin within de novo protein bodies localized within the root storage tissues, resulting in total protein levels of 12.5% dry weight within this tissue, a fourfold increase compared to non-transgenic controls. No significant differences were seen for morphological or agronomic characteristics of transgenic and wild type plants in the greenhouse and field trials, but relative to controls, levels of cyanogenic compounds were reduced by up to 55% in both leaf and root tissues of transgenic plants. Data described here represent a proof of concept towards the potential transformation of cassava from a starchy staple, devoid of storage protein, to one capable of supplying inexpensive, plant-based proteins for food, feed and industrial applications.Mohammad AbharyDimuth SiritungaGene StevensNigel J TaylorClaude M FauquetPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 1, p e16256 (2011)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Mohammad Abhary
Dimuth Siritunga
Gene Stevens
Nigel J Taylor
Claude M Fauquet
Transgenic biofortification of the starchy staple cassava (Manihot esculenta) generates a novel sink for protein.
description Although calorie dense, the starchy, tuberous roots of cassava provide the lowest sources of dietary protein within the major staple food crops (Manihot esculenta Crantz). (Montagnac JA, Davis CR, Tanumihardjo SA. (2009) Compr Rev Food Sci Food Saf 8:181-194). Cassava was genetically modified to express zeolin, a nutritionally balanced storage protein under control of the patatin promoter. Transgenic plants accumulated zeolin within de novo protein bodies localized within the root storage tissues, resulting in total protein levels of 12.5% dry weight within this tissue, a fourfold increase compared to non-transgenic controls. No significant differences were seen for morphological or agronomic characteristics of transgenic and wild type plants in the greenhouse and field trials, but relative to controls, levels of cyanogenic compounds were reduced by up to 55% in both leaf and root tissues of transgenic plants. Data described here represent a proof of concept towards the potential transformation of cassava from a starchy staple, devoid of storage protein, to one capable of supplying inexpensive, plant-based proteins for food, feed and industrial applications.
format article
author Mohammad Abhary
Dimuth Siritunga
Gene Stevens
Nigel J Taylor
Claude M Fauquet
author_facet Mohammad Abhary
Dimuth Siritunga
Gene Stevens
Nigel J Taylor
Claude M Fauquet
author_sort Mohammad Abhary
title Transgenic biofortification of the starchy staple cassava (Manihot esculenta) generates a novel sink for protein.
title_short Transgenic biofortification of the starchy staple cassava (Manihot esculenta) generates a novel sink for protein.
title_full Transgenic biofortification of the starchy staple cassava (Manihot esculenta) generates a novel sink for protein.
title_fullStr Transgenic biofortification of the starchy staple cassava (Manihot esculenta) generates a novel sink for protein.
title_full_unstemmed Transgenic biofortification of the starchy staple cassava (Manihot esculenta) generates a novel sink for protein.
title_sort transgenic biofortification of the starchy staple cassava (manihot esculenta) generates a novel sink for protein.
publisher Public Library of Science (PLoS)
publishDate 2011
url https://doaj.org/article/8a8bdec754df4eef9ce6043416993583
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