Transgenic biofortification of the starchy staple cassava (Manihot esculenta) generates a novel sink for protein.

Although calorie dense, the starchy, tuberous roots of cassava provide the lowest sources of dietary protein within the major staple food crops (Manihot esculenta Crantz). (Montagnac JA, Davis CR, Tanumihardjo SA. (2009) Compr Rev Food Sci Food Saf 8:181-194). Cassava was genetically modified to exp...

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Autores principales:	Mohammad Abhary, Dimuth Siritunga, Gene Stevens, Nigel J Taylor, Claude M Fauquet
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8a8bdec754df4eef9ce6043416993583
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spelling	oai:doaj.org-article:8a8bdec754df4eef9ce60434169935832021-11-18T06:59:54ZTransgenic biofortification of the starchy staple cassava (Manihot esculenta) generates a novel sink for protein.1932-620310.1371/journal.pone.0016256https://doaj.org/article/8a8bdec754df4eef9ce60434169935832011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21283593/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Although calorie dense, the starchy, tuberous roots of cassava provide the lowest sources of dietary protein within the major staple food crops (Manihot esculenta Crantz). (Montagnac JA, Davis CR, Tanumihardjo SA. (2009) Compr Rev Food Sci Food Saf 8:181-194). Cassava was genetically modified to express zeolin, a nutritionally balanced storage protein under control of the patatin promoter. Transgenic plants accumulated zeolin within de novo protein bodies localized within the root storage tissues, resulting in total protein levels of 12.5% dry weight within this tissue, a fourfold increase compared to non-transgenic controls. No significant differences were seen for morphological or agronomic characteristics of transgenic and wild type plants in the greenhouse and field trials, but relative to controls, levels of cyanogenic compounds were reduced by up to 55% in both leaf and root tissues of transgenic plants. Data described here represent a proof of concept towards the potential transformation of cassava from a starchy staple, devoid of storage protein, to one capable of supplying inexpensive, plant-based proteins for food, feed and industrial applications.Mohammad AbharyDimuth SiritungaGene StevensNigel J TaylorClaude M FauquetPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 1, p e16256 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mohammad Abhary Dimuth Siritunga Gene Stevens Nigel J Taylor Claude M Fauquet Transgenic biofortification of the starchy staple cassava (Manihot esculenta) generates a novel sink for protein.
description	Although calorie dense, the starchy, tuberous roots of cassava provide the lowest sources of dietary protein within the major staple food crops (Manihot esculenta Crantz). (Montagnac JA, Davis CR, Tanumihardjo SA. (2009) Compr Rev Food Sci Food Saf 8:181-194). Cassava was genetically modified to express zeolin, a nutritionally balanced storage protein under control of the patatin promoter. Transgenic plants accumulated zeolin within de novo protein bodies localized within the root storage tissues, resulting in total protein levels of 12.5% dry weight within this tissue, a fourfold increase compared to non-transgenic controls. No significant differences were seen for morphological or agronomic characteristics of transgenic and wild type plants in the greenhouse and field trials, but relative to controls, levels of cyanogenic compounds were reduced by up to 55% in both leaf and root tissues of transgenic plants. Data described here represent a proof of concept towards the potential transformation of cassava from a starchy staple, devoid of storage protein, to one capable of supplying inexpensive, plant-based proteins for food, feed and industrial applications.
format	article
author	Mohammad Abhary Dimuth Siritunga Gene Stevens Nigel J Taylor Claude M Fauquet
author_facet	Mohammad Abhary Dimuth Siritunga Gene Stevens Nigel J Taylor Claude M Fauquet
author_sort	Mohammad Abhary
title	Transgenic biofortification of the starchy staple cassava (Manihot esculenta) generates a novel sink for protein.
title_short	Transgenic biofortification of the starchy staple cassava (Manihot esculenta) generates a novel sink for protein.
title_full	Transgenic biofortification of the starchy staple cassava (Manihot esculenta) generates a novel sink for protein.
title_fullStr	Transgenic biofortification of the starchy staple cassava (Manihot esculenta) generates a novel sink for protein.
title_full_unstemmed	Transgenic biofortification of the starchy staple cassava (Manihot esculenta) generates a novel sink for protein.
title_sort	transgenic biofortification of the starchy staple cassava (manihot esculenta) generates a novel sink for protein.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/8a8bdec754df4eef9ce6043416993583
work_keys_str_mv	AT mohammadabhary transgenicbiofortificationofthestarchystaplecassavamanihotesculentageneratesanovelsinkforprotein AT dimuthsiritunga transgenicbiofortificationofthestarchystaplecassavamanihotesculentageneratesanovelsinkforprotein AT genestevens transgenicbiofortificationofthestarchystaplecassavamanihotesculentageneratesanovelsinkforprotein AT nigeljtaylor transgenicbiofortificationofthestarchystaplecassavamanihotesculentageneratesanovelsinkforprotein AT claudemfauquet transgenicbiofortificationofthestarchystaplecassavamanihotesculentageneratesanovelsinkforprotein
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Transgenic biofortification of the starchy staple cassava (Manihot esculenta) generates a novel sink for protein.

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