Reduced spontaneous itch in mouse models of cholestasis

Abstract Pruritus is one of the most distressing symptoms in cholestatic patients. Plasma autotaxin (ATX) activity correlates with the severity of pruritus in cholestatic patients, but the pathophysiology is unclear. To study pruritus in mice, we measured scratch activity in cholestatic Atp8b1 mutan...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jacqueline Langedijk, Ruth Bolier, Dagmar Tolenaars, Lysbeth ten Bloemendaal, Suzanne Duijst, Dirk de Waart, Ulrich Beuers, Piter Bosma, Ronald Oude Elferink
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8a91086451c548c488f3ed16188f6c17
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8a91086451c548c488f3ed16188f6c17
record_format	dspace
spelling	oai:doaj.org-article:8a91086451c548c488f3ed16188f6c172021-12-02T17:05:00ZReduced spontaneous itch in mouse models of cholestasis10.1038/s41598-021-85660-12045-2322https://doaj.org/article/8a91086451c548c488f3ed16188f6c172021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85660-1https://doaj.org/toc/2045-2322Abstract Pruritus is one of the most distressing symptoms in cholestatic patients. Plasma autotaxin (ATX) activity correlates with the severity of pruritus in cholestatic patients, but the pathophysiology is unclear. To study pruritus in mice, we measured scratch activity in cholestatic Atp8b1 mutant mice, a model for Progressive Familial Intrahepatic Cholestasis type 1, and wild type mice (WT) with alpha-naphthylisothiocyanate (ANIT)-induced cholestasis. To induce cholestasis, Atp8b1 mutant mice received a diet containing 0.1% cholic acid (CA) and WT mice were treated with ANIT. In these mice ATX was also overexpressed by transduction with AAV-ATX. Scratch activity was measured using an unbiased, electronic assay. Marked cholestasis was accomplished in both Atp8b1 mutant mice on a CA-supplemented diet and in ANIT-treatment in WT mice, but scratch activity was decreased rather than increased while plasma ATX activity was increased. Plasma ATX activity was further increased up to fivefold with AAV-ATX, but this did not induce scratch activity. In contrast to several reports two cholestatic mouse models did not display increased scratch activity as a measure of itch perception. Increasing plasma ATX activity by overexpression also did not lead to increased scratch activity in mice. This questions whether mice are suitable to study cholestatic itch.Jacqueline LangedijkRuth BolierDagmar TolenaarsLysbeth ten BloemendaalSuzanne DuijstDirk de WaartUlrich BeuersPiter BosmaRonald Oude ElferinkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jacqueline Langedijk Ruth Bolier Dagmar Tolenaars Lysbeth ten Bloemendaal Suzanne Duijst Dirk de Waart Ulrich Beuers Piter Bosma Ronald Oude Elferink Reduced spontaneous itch in mouse models of cholestasis
description	Abstract Pruritus is one of the most distressing symptoms in cholestatic patients. Plasma autotaxin (ATX) activity correlates with the severity of pruritus in cholestatic patients, but the pathophysiology is unclear. To study pruritus in mice, we measured scratch activity in cholestatic Atp8b1 mutant mice, a model for Progressive Familial Intrahepatic Cholestasis type 1, and wild type mice (WT) with alpha-naphthylisothiocyanate (ANIT)-induced cholestasis. To induce cholestasis, Atp8b1 mutant mice received a diet containing 0.1% cholic acid (CA) and WT mice were treated with ANIT. In these mice ATX was also overexpressed by transduction with AAV-ATX. Scratch activity was measured using an unbiased, electronic assay. Marked cholestasis was accomplished in both Atp8b1 mutant mice on a CA-supplemented diet and in ANIT-treatment in WT mice, but scratch activity was decreased rather than increased while plasma ATX activity was increased. Plasma ATX activity was further increased up to fivefold with AAV-ATX, but this did not induce scratch activity. In contrast to several reports two cholestatic mouse models did not display increased scratch activity as a measure of itch perception. Increasing plasma ATX activity by overexpression also did not lead to increased scratch activity in mice. This questions whether mice are suitable to study cholestatic itch.
format	article
author	Jacqueline Langedijk Ruth Bolier Dagmar Tolenaars Lysbeth ten Bloemendaal Suzanne Duijst Dirk de Waart Ulrich Beuers Piter Bosma Ronald Oude Elferink
author_facet	Jacqueline Langedijk Ruth Bolier Dagmar Tolenaars Lysbeth ten Bloemendaal Suzanne Duijst Dirk de Waart Ulrich Beuers Piter Bosma Ronald Oude Elferink
author_sort	Jacqueline Langedijk
title	Reduced spontaneous itch in mouse models of cholestasis
title_short	Reduced spontaneous itch in mouse models of cholestasis
title_full	Reduced spontaneous itch in mouse models of cholestasis
title_fullStr	Reduced spontaneous itch in mouse models of cholestasis
title_full_unstemmed	Reduced spontaneous itch in mouse models of cholestasis
title_sort	reduced spontaneous itch in mouse models of cholestasis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8a91086451c548c488f3ed16188f6c17
work_keys_str_mv	AT jacquelinelangedijk reducedspontaneousitchinmousemodelsofcholestasis AT ruthbolier reducedspontaneousitchinmousemodelsofcholestasis AT dagmartolenaars reducedspontaneousitchinmousemodelsofcholestasis AT lysbethtenbloemendaal reducedspontaneousitchinmousemodelsofcholestasis AT suzanneduijst reducedspontaneousitchinmousemodelsofcholestasis AT dirkdewaart reducedspontaneousitchinmousemodelsofcholestasis AT ulrichbeuers reducedspontaneousitchinmousemodelsofcholestasis AT piterbosma reducedspontaneousitchinmousemodelsofcholestasis AT ronaldoudeelferink reducedspontaneousitchinmousemodelsofcholestasis
_version_	1718381801821437952

Reduced spontaneous itch in mouse models of cholestasis

Ejemplares similares