Characterization of Burkholderia rhizoxinica and B. endofungorum isolated from clinical specimens.

Eight isolates submitted to CDC from 1989 to 2006 from clinical specimens were initially identified as members of the genus Burkholderia based on preliminary cellular fatty acid analysis and/or 16S rRNA gene sequencing. With the recent descriptions of the new species B. rhizoxinica and B. endofungor...

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Autores principales: Jay E Gee, Mindy B Glass, Gerald Lackner, Leta O Helsel, Maryam Daneshvar, Dannie G Hollis, Jean Jordan, Roger Morey, Arnold Steigerwalt, Christian Hertweck
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Publicado: Public Library of Science (PLoS) 2011
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spelling oai:doaj.org-article:8a9776117bf442699b79effe5af7d1e42021-11-18T07:00:20ZCharacterization of Burkholderia rhizoxinica and B. endofungorum isolated from clinical specimens.1932-620310.1371/journal.pone.0015731https://doaj.org/article/8a9776117bf442699b79effe5af7d1e42011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21267449/?tool=EBIhttps://doaj.org/toc/1932-6203Eight isolates submitted to CDC from 1989 to 2006 from clinical specimens were initially identified as members of the genus Burkholderia based on preliminary cellular fatty acid analysis and/or 16S rRNA gene sequencing. With the recent descriptions of the new species B. rhizoxinica and B. endofungorum, which are considered endosymbiotic bacteria in Rhizopus microsporus fungi, we now identify seven of these clinical isolates as B. rhizoxinica and one as B. endofungorum based on biochemical testing, 16s rRNA, and DNA-DNA hybridization results. We also further characterize these isolates by assessing toxin production and/or by multiple locus sequence typing.Jay E GeeMindy B GlassGerald LacknerLeta O HelselMaryam DaneshvarDannie G HollisJean JordanRoger MoreyArnold SteigerwaltChristian HertweckPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 1, p e15731 (2011)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Jay E Gee
Mindy B Glass
Gerald Lackner
Leta O Helsel
Maryam Daneshvar
Dannie G Hollis
Jean Jordan
Roger Morey
Arnold Steigerwalt
Christian Hertweck
Characterization of Burkholderia rhizoxinica and B. endofungorum isolated from clinical specimens.
description Eight isolates submitted to CDC from 1989 to 2006 from clinical specimens were initially identified as members of the genus Burkholderia based on preliminary cellular fatty acid analysis and/or 16S rRNA gene sequencing. With the recent descriptions of the new species B. rhizoxinica and B. endofungorum, which are considered endosymbiotic bacteria in Rhizopus microsporus fungi, we now identify seven of these clinical isolates as B. rhizoxinica and one as B. endofungorum based on biochemical testing, 16s rRNA, and DNA-DNA hybridization results. We also further characterize these isolates by assessing toxin production and/or by multiple locus sequence typing.
format article
author Jay E Gee
Mindy B Glass
Gerald Lackner
Leta O Helsel
Maryam Daneshvar
Dannie G Hollis
Jean Jordan
Roger Morey
Arnold Steigerwalt
Christian Hertweck
author_facet Jay E Gee
Mindy B Glass
Gerald Lackner
Leta O Helsel
Maryam Daneshvar
Dannie G Hollis
Jean Jordan
Roger Morey
Arnold Steigerwalt
Christian Hertweck
author_sort Jay E Gee
title Characterization of Burkholderia rhizoxinica and B. endofungorum isolated from clinical specimens.
title_short Characterization of Burkholderia rhizoxinica and B. endofungorum isolated from clinical specimens.
title_full Characterization of Burkholderia rhizoxinica and B. endofungorum isolated from clinical specimens.
title_fullStr Characterization of Burkholderia rhizoxinica and B. endofungorum isolated from clinical specimens.
title_full_unstemmed Characterization of Burkholderia rhizoxinica and B. endofungorum isolated from clinical specimens.
title_sort characterization of burkholderia rhizoxinica and b. endofungorum isolated from clinical specimens.
publisher Public Library of Science (PLoS)
publishDate 2011
url https://doaj.org/article/8a9776117bf442699b79effe5af7d1e4
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