Low-Magnitude High-Frequency Vibration Decreases Body Weight Gain and Increases Muscle Strength by Enhancing the p38 and AMPK Pathways in db/db Mice

Zhitao Ren,1 Qingping Lan,1 Yan Chen,1 Yuet Wa Judy Chan,1 Gail B Mahady,2 Simon Ming-Yuen Lee1 1State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macau, People’s Republic of China; 2Department of Pharmacy Practice,...

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Autores principales:	Ren Z, Lan Q, Chen Y, Chan YWJ, Mahady GB, Lee SMY
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	type 2 diabetes mellitus low-magnitude high frequency vibration mitochondrial biogenesis ampkα p38; Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/8a9f40f637ce479da4220dabbf0c135f
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spelling	oai:doaj.org-article:8a9f40f637ce479da4220dabbf0c135f2021-12-02T11:43:36ZLow-Magnitude High-Frequency Vibration Decreases Body Weight Gain and Increases Muscle Strength by Enhancing the p38 and AMPK Pathways in db/db Mice1178-7007https://doaj.org/article/8a9f40f637ce479da4220dabbf0c135f2020-04-01T00:00:00Zhttps://www.dovepress.com/low-magnitude-high-frequency-vibration-decreases-body-weight-gain-and--peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Zhitao Ren,1 Qingping Lan,1 Yan Chen,1 Yuet Wa Judy Chan,1 Gail B Mahady,2 Simon Ming-Yuen Lee1 1State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macau, People’s Republic of China; 2Department of Pharmacy Practice, College of Pharmacy, PAHO/WHO Collaborating Center for Traditional Medicine, University of Illinois at Chicago, Chicago, IL, USACorrespondence: Simon Ming-Yuen LeeInstitute of Chinese Medical Sciences, University of Macau, Avenide Da Universidade, Room 7003, N22 Building, Taipa, Macau, People’s Republic of ChinaTel +853-88224695Email simonlee@umac.moObjective: To evaluate the effect LMHFV on body weight gain, NAFLD and muscle strength and explore effect in mitochondrial biogenesis, AMPKα and p38 pathways.Methods: Vibration platform used in this study provides specific whole-body cyclic mechanical stimulation at low magnitude (0.3 g) and high frequency (50 Hz). Diabetic mice (8– 9 mice per group) (C57BL/KsJ-m+/+Leprdb) were randomly divided into untreated group (no vibration) and two vibration groups. Lean mice (8 mice) were used as non-diabetic control for both groups. Two diabetic vibration groups received LMHFV every day for 20 min/day and 40 min/day separately.Results: After 8 weeks of treatment, results showed that body weight, liver weight, fat pad weight, glucose level and insulin level were lower in vibration group when compared with the untreated group. The ratio of fat in liver was significantly decreased after vibration treatment. Muscle strength was significantly increased after vibration. Mitochondrial biogenesis-related gene expression was increased in soleus, gastrocnemius and liver. AMPKα mRNA expression level was increased in soleus and gastrocnemius after vibration treatment. p38 and AMPKα mRNA expression level and protein expression level in liver were enhanced with vibration treatment. Moreover, phosphorylation of p38 and AMPKα was enhanced in liver.Conclusion: LMHFV applied in our study decreases body weight gain and improves muscle strength and NAFLD in diabetic mice which were partly through improving mitochondrial biogenesis by enhancing p38 and AMPKα pathway.Keywords: type 2 diabetes mellitus, low-magnitude high-frequency vibration, mitochondrial biogenesis, AMPKα, p38Ren ZLan QChen YChan YWJMahady GBLee SMYDove Medical Pressarticletype 2 diabetes mellituslow-magnitude high frequency vibrationmitochondrial biogenesisampkαp38;Specialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 979-989 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	type 2 diabetes mellitus low-magnitude high frequency vibration mitochondrial biogenesis ampkα p38; Specialties of internal medicine RC581-951
spellingShingle	type 2 diabetes mellitus low-magnitude high frequency vibration mitochondrial biogenesis ampkα p38; Specialties of internal medicine RC581-951 Ren Z Lan Q Chen Y Chan YWJ Mahady GB Lee SMY Low-Magnitude High-Frequency Vibration Decreases Body Weight Gain and Increases Muscle Strength by Enhancing the p38 and AMPK Pathways in db/db Mice
description	Zhitao Ren,1 Qingping Lan,1 Yan Chen,1 Yuet Wa Judy Chan,1 Gail B Mahady,2 Simon Ming-Yuen Lee1 1State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macau, People’s Republic of China; 2Department of Pharmacy Practice, College of Pharmacy, PAHO/WHO Collaborating Center for Traditional Medicine, University of Illinois at Chicago, Chicago, IL, USACorrespondence: Simon Ming-Yuen LeeInstitute of Chinese Medical Sciences, University of Macau, Avenide Da Universidade, Room 7003, N22 Building, Taipa, Macau, People’s Republic of ChinaTel +853-88224695Email simonlee@umac.moObjective: To evaluate the effect LMHFV on body weight gain, NAFLD and muscle strength and explore effect in mitochondrial biogenesis, AMPKα and p38 pathways.Methods: Vibration platform used in this study provides specific whole-body cyclic mechanical stimulation at low magnitude (0.3 g) and high frequency (50 Hz). Diabetic mice (8– 9 mice per group) (C57BL/KsJ-m+/+Leprdb) were randomly divided into untreated group (no vibration) and two vibration groups. Lean mice (8 mice) were used as non-diabetic control for both groups. Two diabetic vibration groups received LMHFV every day for 20 min/day and 40 min/day separately.Results: After 8 weeks of treatment, results showed that body weight, liver weight, fat pad weight, glucose level and insulin level were lower in vibration group when compared with the untreated group. The ratio of fat in liver was significantly decreased after vibration treatment. Muscle strength was significantly increased after vibration. Mitochondrial biogenesis-related gene expression was increased in soleus, gastrocnemius and liver. AMPKα mRNA expression level was increased in soleus and gastrocnemius after vibration treatment. p38 and AMPKα mRNA expression level and protein expression level in liver were enhanced with vibration treatment. Moreover, phosphorylation of p38 and AMPKα was enhanced in liver.Conclusion: LMHFV applied in our study decreases body weight gain and improves muscle strength and NAFLD in diabetic mice which were partly through improving mitochondrial biogenesis by enhancing p38 and AMPKα pathway.Keywords: type 2 diabetes mellitus, low-magnitude high-frequency vibration, mitochondrial biogenesis, AMPKα, p38
format	article
author	Ren Z Lan Q Chen Y Chan YWJ Mahady GB Lee SMY
author_facet	Ren Z Lan Q Chen Y Chan YWJ Mahady GB Lee SMY
author_sort	Ren Z
title	Low-Magnitude High-Frequency Vibration Decreases Body Weight Gain and Increases Muscle Strength by Enhancing the p38 and AMPK Pathways in db/db Mice
title_short	Low-Magnitude High-Frequency Vibration Decreases Body Weight Gain and Increases Muscle Strength by Enhancing the p38 and AMPK Pathways in db/db Mice
title_full	Low-Magnitude High-Frequency Vibration Decreases Body Weight Gain and Increases Muscle Strength by Enhancing the p38 and AMPK Pathways in db/db Mice
title_fullStr	Low-Magnitude High-Frequency Vibration Decreases Body Weight Gain and Increases Muscle Strength by Enhancing the p38 and AMPK Pathways in db/db Mice
title_full_unstemmed	Low-Magnitude High-Frequency Vibration Decreases Body Weight Gain and Increases Muscle Strength by Enhancing the p38 and AMPK Pathways in db/db Mice
title_sort	low-magnitude high-frequency vibration decreases body weight gain and increases muscle strength by enhancing the p38 and ampk pathways in db/db mice
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/8a9f40f637ce479da4220dabbf0c135f
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Low-Magnitude High-Frequency Vibration Decreases Body Weight Gain and Increases Muscle Strength by Enhancing the p38 and AMPK Pathways in db/db Mice

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