Lactotransferrin in Asian elephant (Elephas maximus) seminal plasma correlates with semen quality.

Asian elephants (Elephas maximus) have highly variable ejaculate quality within individuals, greatly reducing the efficacy of artificial insemination and making it difficult to devise a sperm cryopreservation protocol for this endangered species. Because seminal plasma influences sperm function and...

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Autores principales:	Wendy K Kiso, Vimal Selvaraj, Jennifer Nagashima, Atsushi Asano, Janine L Brown, Dennis L Schmitt, John Leszyk, Alexander J Travis, Budhan S Pukazhenthi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8aa9ef5179d04865922dfebd1de543c6
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spelling	oai:doaj.org-article:8aa9ef5179d04865922dfebd1de543c62021-11-18T08:59:19ZLactotransferrin in Asian elephant (Elephas maximus) seminal plasma correlates with semen quality.1932-620310.1371/journal.pone.0071033https://doaj.org/article/8aa9ef5179d04865922dfebd1de543c62013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23976974/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Asian elephants (Elephas maximus) have highly variable ejaculate quality within individuals, greatly reducing the efficacy of artificial insemination and making it difficult to devise a sperm cryopreservation protocol for this endangered species. Because seminal plasma influences sperm function and physiology, including sperm motility, the objectives of this study were to characterize the chemistry and protein profiles of Asian elephant seminal plasma and to determine the relationships between seminal plasma components and semen quality. Ejaculates exhibiting good sperm motility (≥65%) expressed higher percentages of spermatozoa with normal morphology (80.3±13.0 vs. 44.9±30.8%) and positive Spermac staining (51.9±14.5 vs. 7.5±14.4%), in addition to higher total volume (135.1±89.6 vs. 88.8±73.1 ml) and lower sperm concentration (473.0±511.2 vs. 1313.8±764.7×10⁶ cells ml⁻¹) compared to ejaculates exhibiting poor sperm motility (≤10%; P<0.05). Comparison of seminal plasma from ejaculates with good versus poor sperm motility revealed significant differences in concentrations of creatine phosphokinase, alanine aminotransferase, phosphorus, sodium, chloride, magnesium, and glucose. These observations suggest seminal plasma influences semen quality in elephants. One- and two-dimensional (2D) gel electrophoresis revealed largely similar compositional profiles of seminal plasma proteins between good and poor motility ejaculates. However, a protein of ∼80 kDa was abundant in 85% of ejaculates with good motility, and was absent in 90% of poor motility ejaculates (P<0.05). We used mass spectrometry to identify this protein as lactotransferrin, and immunoblot analysis to confirm this identification. Together, these findings lay a functional foundation for understanding the contributions of seminal plasma in the regulation of Asian elephant sperm motility, and for improving semen collection and storage in this endangered species.Wendy K KisoVimal SelvarajJennifer NagashimaAtsushi AsanoJanine L BrownDennis L SchmittJohn LeszykAlexander J TravisBudhan S PukazhenthiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e71033 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wendy K Kiso Vimal Selvaraj Jennifer Nagashima Atsushi Asano Janine L Brown Dennis L Schmitt John Leszyk Alexander J Travis Budhan S Pukazhenthi Lactotransferrin in Asian elephant (Elephas maximus) seminal plasma correlates with semen quality.
description	Asian elephants (Elephas maximus) have highly variable ejaculate quality within individuals, greatly reducing the efficacy of artificial insemination and making it difficult to devise a sperm cryopreservation protocol for this endangered species. Because seminal plasma influences sperm function and physiology, including sperm motility, the objectives of this study were to characterize the chemistry and protein profiles of Asian elephant seminal plasma and to determine the relationships between seminal plasma components and semen quality. Ejaculates exhibiting good sperm motility (≥65%) expressed higher percentages of spermatozoa with normal morphology (80.3±13.0 vs. 44.9±30.8%) and positive Spermac staining (51.9±14.5 vs. 7.5±14.4%), in addition to higher total volume (135.1±89.6 vs. 88.8±73.1 ml) and lower sperm concentration (473.0±511.2 vs. 1313.8±764.7×10⁶ cells ml⁻¹) compared to ejaculates exhibiting poor sperm motility (≤10%; P<0.05). Comparison of seminal plasma from ejaculates with good versus poor sperm motility revealed significant differences in concentrations of creatine phosphokinase, alanine aminotransferase, phosphorus, sodium, chloride, magnesium, and glucose. These observations suggest seminal plasma influences semen quality in elephants. One- and two-dimensional (2D) gel electrophoresis revealed largely similar compositional profiles of seminal plasma proteins between good and poor motility ejaculates. However, a protein of ∼80 kDa was abundant in 85% of ejaculates with good motility, and was absent in 90% of poor motility ejaculates (P<0.05). We used mass spectrometry to identify this protein as lactotransferrin, and immunoblot analysis to confirm this identification. Together, these findings lay a functional foundation for understanding the contributions of seminal plasma in the regulation of Asian elephant sperm motility, and for improving semen collection and storage in this endangered species.
format	article
author	Wendy K Kiso Vimal Selvaraj Jennifer Nagashima Atsushi Asano Janine L Brown Dennis L Schmitt John Leszyk Alexander J Travis Budhan S Pukazhenthi
author_facet	Wendy K Kiso Vimal Selvaraj Jennifer Nagashima Atsushi Asano Janine L Brown Dennis L Schmitt John Leszyk Alexander J Travis Budhan S Pukazhenthi
author_sort	Wendy K Kiso
title	Lactotransferrin in Asian elephant (Elephas maximus) seminal plasma correlates with semen quality.
title_short	Lactotransferrin in Asian elephant (Elephas maximus) seminal plasma correlates with semen quality.
title_full	Lactotransferrin in Asian elephant (Elephas maximus) seminal plasma correlates with semen quality.
title_fullStr	Lactotransferrin in Asian elephant (Elephas maximus) seminal plasma correlates with semen quality.
title_full_unstemmed	Lactotransferrin in Asian elephant (Elephas maximus) seminal plasma correlates with semen quality.
title_sort	lactotransferrin in asian elephant (elephas maximus) seminal plasma correlates with semen quality.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/8aa9ef5179d04865922dfebd1de543c6
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Lactotransferrin in Asian elephant (Elephas maximus) seminal plasma correlates with semen quality.

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