Genetic diversity and population structure of the Bacillus cereus group bacteria from diverse marine environments

Abstract The phylogenetic diversity of marine bacteria belonged to the Bacillus cereus group has not been well investigated. Here, we present the genetic diversity and population structure of 71 bacteria from diverse marine environments, using a multilocus sequence typing (MLST) approach and the ana...

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Autores principales:	Yang Liu, Qiliang Lai, Juan Du, Zongze Shao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8aabf1e311084c668a56427e10c6e460
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spelling	oai:doaj.org-article:8aabf1e311084c668a56427e10c6e4602021-12-02T15:04:56ZGenetic diversity and population structure of the Bacillus cereus group bacteria from diverse marine environments10.1038/s41598-017-00817-12045-2322https://doaj.org/article/8aabf1e311084c668a56427e10c6e4602017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00817-1https://doaj.org/toc/2045-2322Abstract The phylogenetic diversity of marine bacteria belonged to the Bacillus cereus group has not been well investigated. Here, we present the genetic diversity and population structure of 71 bacteria from diverse marine environments, using a multilocus sequence typing (MLST) approach and the analyses of digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) based on some representative genomic sequences. The MLST analysis demonstrated that these isolates were highly diverse and a wide distribution in marine environments and some of them showed niche specificity to some extent. They were assigned to 27 sequence types (STs) with 23 novel STs. Phylogenetic analysis of 82 bacteria containing 11 type strains based on MLST discriminated them as 20 clusters including 10 new ones. Both the dDDH and ANI results supported the proposition that each of 20 clusters represented one independent species, including 10 putative novel species. Values of 98.3% of MLST similarity and 96.2% of ANI were proposed as the standard for the species definition of this group. In summary, the first insight into the phylogenetic diversity of the group bacteria from marine environments will contribute to better understanding of their ecological role and evolution in contrast with terrestrial environments.Yang LiuQiliang LaiJuan DuZongze ShaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yang Liu Qiliang Lai Juan Du Zongze Shao Genetic diversity and population structure of the Bacillus cereus group bacteria from diverse marine environments
description	Abstract The phylogenetic diversity of marine bacteria belonged to the Bacillus cereus group has not been well investigated. Here, we present the genetic diversity and population structure of 71 bacteria from diverse marine environments, using a multilocus sequence typing (MLST) approach and the analyses of digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) based on some representative genomic sequences. The MLST analysis demonstrated that these isolates were highly diverse and a wide distribution in marine environments and some of them showed niche specificity to some extent. They were assigned to 27 sequence types (STs) with 23 novel STs. Phylogenetic analysis of 82 bacteria containing 11 type strains based on MLST discriminated them as 20 clusters including 10 new ones. Both the dDDH and ANI results supported the proposition that each of 20 clusters represented one independent species, including 10 putative novel species. Values of 98.3% of MLST similarity and 96.2% of ANI were proposed as the standard for the species definition of this group. In summary, the first insight into the phylogenetic diversity of the group bacteria from marine environments will contribute to better understanding of their ecological role and evolution in contrast with terrestrial environments.
format	article
author	Yang Liu Qiliang Lai Juan Du Zongze Shao
author_facet	Yang Liu Qiliang Lai Juan Du Zongze Shao
author_sort	Yang Liu
title	Genetic diversity and population structure of the Bacillus cereus group bacteria from diverse marine environments
title_short	Genetic diversity and population structure of the Bacillus cereus group bacteria from diverse marine environments
title_full	Genetic diversity and population structure of the Bacillus cereus group bacteria from diverse marine environments
title_fullStr	Genetic diversity and population structure of the Bacillus cereus group bacteria from diverse marine environments
title_full_unstemmed	Genetic diversity and population structure of the Bacillus cereus group bacteria from diverse marine environments
title_sort	genetic diversity and population structure of the bacillus cereus group bacteria from diverse marine environments
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8aabf1e311084c668a56427e10c6e460
work_keys_str_mv	AT yangliu geneticdiversityandpopulationstructureofthebacilluscereusgroupbacteriafromdiversemarineenvironments AT qilianglai geneticdiversityandpopulationstructureofthebacilluscereusgroupbacteriafromdiversemarineenvironments AT juandu geneticdiversityandpopulationstructureofthebacilluscereusgroupbacteriafromdiversemarineenvironments AT zongzeshao geneticdiversityandpopulationstructureofthebacilluscereusgroupbacteriafromdiversemarineenvironments
_version_	1718388968195620864

Genetic diversity and population structure of the Bacillus cereus group bacteria from diverse marine environments

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