Complement Profiles in Patients with Amyotrophic Lateral Sclerosis: A Prospective Observational Cohort Study

Anne-Lene Kjældgaard,1,2 Katrine Pilely,1 Karsten Skovgaard Olsen,2 Anne Øberg Lauritsen,2 Stephen Wørlich Pedersen,3 Kirsten Svenstrup,3,4 Merete Karlsborg,4 Helle Thagesen,5 Morten Blaabjerg,5 Ásta Theódórsdóttir,6 Elisabe...

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Autores principales: Kjældgaard AL, Pilely K, Olsen KS, Øberg Lauritsen A, Wørlich Pedersen S, Svenstrup K, Karlsborg M, Thagesen H, Blaabjerg M, Theódórsdóttir Á, Gundtoft Elmo E, Torvin Møller A, Pedersen NA, Kirkegaard N, Møller K, Garred P
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Lenguaje:EN
Publicado: Dove Medical Press 2021
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Acceso en línea:https://doaj.org/article/8aaef6c5c7044c7789d2318a4cf8b7b7
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id oai:doaj.org-article:8aaef6c5c7044c7789d2318a4cf8b7b7
record_format dspace
institution DOAJ
collection DOAJ
language EN
topic amyotrophic lateral sclerosis
innate immunity
complement
lectin pathway
cerebrospinal fluid
pathophysiology
observational cohort study
Pathology
RB1-214
Therapeutics. Pharmacology
RM1-950
spellingShingle amyotrophic lateral sclerosis
innate immunity
complement
lectin pathway
cerebrospinal fluid
pathophysiology
observational cohort study
Pathology
RB1-214
Therapeutics. Pharmacology
RM1-950
Kjældgaard AL
Pilely K
Olsen KS
Øberg Lauritsen A
Wørlich Pedersen S
Svenstrup K
Karlsborg M
Thagesen H
Blaabjerg M
Theódórsdóttir Á
Gundtoft Elmo E
Torvin Møller A
Pedersen NA
Kirkegaard N
Møller K
Garred P
Complement Profiles in Patients with Amyotrophic Lateral Sclerosis: A Prospective Observational Cohort Study
description Anne-Lene Kjældgaard,1,2 Katrine Pilely,1 Karsten Skovgaard Olsen,2 Anne Øberg Lauritsen,2 Stephen Wørlich Pedersen,3 Kirsten Svenstrup,3,4 Merete Karlsborg,4 Helle Thagesen,5 Morten Blaabjerg,5 Ásta Theódórsdóttir,6 Elisabeth Gundtoft Elmo,3 Anette Torvin Møller,7 Niels Anker Pedersen,8 Niels Kirkegaard,8 Kirsten Møller,2,9 Peter Garred1,9 1Laboratory of Molecular Medicine, Department of Clinical Immunology, Section 7631, Diagnostic Centre, Rigshospitalet, Copenhagen, Denmark; 2Department of Neuroanaesthesiology Neuroscience Centre, Rigshospitalet, Copenhagen, Denmark; 3Department of Neurology, Neuroscience Centre, Rigshospitalet, Copenhagen, Denmark; 4Department of Neurology, Bispebjerg Hospital, Copenhagen, Denmark; 5Department of Neurology, Roskilde University Hospital, Roskilde, Denmark; 6Department of Neurology, Odense University Hospital, Odense, Denmark; 7Department of Neurology, Aarhus Hospital, Copenhagen, Denmark; 8Department of Anaesthesiology, Private Hospital Gildhøj, Brondby, Denmark; 9Institute of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, DenmarkCorrespondence: Anne-Lene KjældgaardLaboratory of Molecular Medicine, Department of Clinical Immunology, Section 7631, Diagnostic Centre, Rigshospitalet, Ole Maaloesvej 26, Copenhagen, DK-2200, DenmarkTel +45 35457631Fax +45 35398766Email anne-lene.kjaeldgaard@regionh.dkBackground: The complement system has been suggested to be involved in the pathophysiology of amyotrophic lateral sclerosis (ALS), a progressive motor neuron disease. In the present study, we compared levels of selected complement markers to clinical outcome in ALS patients.Methods: This observational, explorative cohort study included 92 ALS patients, 61 neurological controls (NCs) admitted for suspected aneurysmal subarachnoid haemorrhage, and 96 neurologically healthy controls (NHCs). Peripheral blood and cerebrospinal fluid (CSF) were obtained for the measurement of ficolin-1, − 2, and − 3; collectin-11, MBL, MASP-3, MAP-1, C4, C3, PTX-3, and complement activation products C4c, C3bc, and sC5b-9. We recorded clinical outcomes of ALS patients for 24 to 48 months after inclusion in order to analyse the effects of the complement markers on survival time.Results: Compared with both control groups, ALS patients exhibited increased collectin-11, C4 and sC5b-9 in plasma, as well as increased ficolin-3 in CSF. Ficolin-2 was significantly decreased in plasma of the ALS patients compared with NHCs, but not with NCs. The concentration of collectin-11, C3 and C3bc correlated negatively with the revised ALS functional rating scale (ALSFRS-R). No association was found between levels of complement markers and survival as estimated by hazard ratios.Conclusion: ALS patients exhibit aberrant expression of selected mediators of the lectin complement pathway as well as increased activation of the terminal complement pathway, corroborating the notion that the complement system might be involved in the pathophysiology of ALS.Keywords: amyotrophic lateral sclerosis, innate immunity, complement, lectin pathway, cerebrospinal fluid, pathophysiology, observational cohort study
format article
author Kjældgaard AL
Pilely K
Olsen KS
Øberg Lauritsen A
Wørlich Pedersen S
Svenstrup K
Karlsborg M
Thagesen H
Blaabjerg M
Theódórsdóttir Á
Gundtoft Elmo E
Torvin Møller A
Pedersen NA
Kirkegaard N
Møller K
Garred P
author_facet Kjældgaard AL
Pilely K
Olsen KS
Øberg Lauritsen A
Wørlich Pedersen S
Svenstrup K
Karlsborg M
Thagesen H
Blaabjerg M
Theódórsdóttir Á
Gundtoft Elmo E
Torvin Møller A
Pedersen NA
Kirkegaard N
Møller K
Garred P
author_sort Kjældgaard AL
title Complement Profiles in Patients with Amyotrophic Lateral Sclerosis: A Prospective Observational Cohort Study
title_short Complement Profiles in Patients with Amyotrophic Lateral Sclerosis: A Prospective Observational Cohort Study
title_full Complement Profiles in Patients with Amyotrophic Lateral Sclerosis: A Prospective Observational Cohort Study
title_fullStr Complement Profiles in Patients with Amyotrophic Lateral Sclerosis: A Prospective Observational Cohort Study
title_full_unstemmed Complement Profiles in Patients with Amyotrophic Lateral Sclerosis: A Prospective Observational Cohort Study
title_sort complement profiles in patients with amyotrophic lateral sclerosis: a prospective observational cohort study
publisher Dove Medical Press
publishDate 2021
url https://doaj.org/article/8aaef6c5c7044c7789d2318a4cf8b7b7
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spelling oai:doaj.org-article:8aaef6c5c7044c7789d2318a4cf8b7b72021-12-02T13:13:15ZComplement Profiles in Patients with Amyotrophic Lateral Sclerosis: A Prospective Observational Cohort Study1178-7031https://doaj.org/article/8aaef6c5c7044c7789d2318a4cf8b7b72021-03-01T00:00:00Zhttps://www.dovepress.com/complement-profiles-in-patients-with-amyotrophic-lateral-sclerosis-a-p-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Anne-Lene Kjældgaard,1,2 Katrine Pilely,1 Karsten Skovgaard Olsen,2 Anne Øberg Lauritsen,2 Stephen Wørlich Pedersen,3 Kirsten Svenstrup,3,4 Merete Karlsborg,4 Helle Thagesen,5 Morten Blaabjerg,5 Ásta Theódórsdóttir,6 Elisabeth Gundtoft Elmo,3 Anette Torvin Møller,7 Niels Anker Pedersen,8 Niels Kirkegaard,8 Kirsten Møller,2,9 Peter Garred1,9 1Laboratory of Molecular Medicine, Department of Clinical Immunology, Section 7631, Diagnostic Centre, Rigshospitalet, Copenhagen, Denmark; 2Department of Neuroanaesthesiology Neuroscience Centre, Rigshospitalet, Copenhagen, Denmark; 3Department of Neurology, Neuroscience Centre, Rigshospitalet, Copenhagen, Denmark; 4Department of Neurology, Bispebjerg Hospital, Copenhagen, Denmark; 5Department of Neurology, Roskilde University Hospital, Roskilde, Denmark; 6Department of Neurology, Odense University Hospital, Odense, Denmark; 7Department of Neurology, Aarhus Hospital, Copenhagen, Denmark; 8Department of Anaesthesiology, Private Hospital Gildhøj, Brondby, Denmark; 9Institute of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, DenmarkCorrespondence: Anne-Lene KjældgaardLaboratory of Molecular Medicine, Department of Clinical Immunology, Section 7631, Diagnostic Centre, Rigshospitalet, Ole Maaloesvej 26, Copenhagen, DK-2200, DenmarkTel +45 35457631Fax +45 35398766Email anne-lene.kjaeldgaard@regionh.dkBackground: The complement system has been suggested to be involved in the pathophysiology of amyotrophic lateral sclerosis (ALS), a progressive motor neuron disease. In the present study, we compared levels of selected complement markers to clinical outcome in ALS patients.Methods: This observational, explorative cohort study included 92 ALS patients, 61 neurological controls (NCs) admitted for suspected aneurysmal subarachnoid haemorrhage, and 96 neurologically healthy controls (NHCs). Peripheral blood and cerebrospinal fluid (CSF) were obtained for the measurement of ficolin-1, − 2, and − 3; collectin-11, MBL, MASP-3, MAP-1, C4, C3, PTX-3, and complement activation products C4c, C3bc, and sC5b-9. We recorded clinical outcomes of ALS patients for 24 to 48 months after inclusion in order to analyse the effects of the complement markers on survival time.Results: Compared with both control groups, ALS patients exhibited increased collectin-11, C4 and sC5b-9 in plasma, as well as increased ficolin-3 in CSF. Ficolin-2 was significantly decreased in plasma of the ALS patients compared with NHCs, but not with NCs. The concentration of collectin-11, C3 and C3bc correlated negatively with the revised ALS functional rating scale (ALSFRS-R). No association was found between levels of complement markers and survival as estimated by hazard ratios.Conclusion: ALS patients exhibit aberrant expression of selected mediators of the lectin complement pathway as well as increased activation of the terminal complement pathway, corroborating the notion that the complement system might be involved in the pathophysiology of ALS.Keywords: amyotrophic lateral sclerosis, innate immunity, complement, lectin pathway, cerebrospinal fluid, pathophysiology, observational cohort studyKjældgaard ALPilely KOlsen KSØberg Lauritsen AWørlich Pedersen SSvenstrup KKarlsborg MThagesen HBlaabjerg MTheódórsdóttir ÁGundtoft Elmo ETorvin Møller APedersen NAKirkegaard NMøller KGarred PDove Medical Pressarticleamyotrophic lateral sclerosisinnate immunitycomplementlectin pathwaycerebrospinal fluidpathophysiologyobservational cohort studyPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 1043-1053 (2021)