Osteogenic capacity of nanocrystalline bone cement in a weight-bearing defect at the ovine tibial metaphysis

Christoph Harms,1 Kai Helms,1 Tibor Taschner,1 Ioannis Stratos,1 Anita Ignatius,5 Thomas Gerber,2 Solvig Lenz,3 Stefan Rammelt,6 Brigitte Vollmar,4 Thomas Mittlmeier11Department of Trauma and Reconstructive Surgery, 2Department for Materials Research and Nanostructures, Institute for Physics, 3Depar...

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Autores principales:	Mittlmeier T, Vollmar B, Rammelt S, Lenz S, Gerber T, Ignatius A, Stratos I, Taschner T, Helms K, Harms C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8ab9c875a2134d438adf096231026eb3
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spelling	oai:doaj.org-article:8ab9c875a2134d438adf096231026eb32021-12-02T05:02:11ZOsteogenic capacity of nanocrystalline bone cement in a weight-bearing defect at the ovine tibial metaphysis1176-91141178-2013https://doaj.org/article/8ab9c875a2134d438adf096231026eb32012-06-01T00:00:00Zhttp://www.dovepress.com/osteogenic-capacity-of-nanocrystalline-bone-cement-in-a-weight-bearing-a10114https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Christoph Harms,1 Kai Helms,1 Tibor Taschner,1 Ioannis Stratos,1 Anita Ignatius,5 Thomas Gerber,2 Solvig Lenz,3 Stefan Rammelt,6 Brigitte Vollmar,4 Thomas Mittlmeier11Department of Trauma and Reconstructive Surgery, 2Department for Materials Research and Nanostructures, Institute for Physics, 3Department of Oral and Maxillofacial Plastic Surgery, 4Institute for Experimental Surgery, University of Rostock, Rostock, 5Institute of Orthopaedic Research and Biomechanics, University of Ulm, Ulm, 6Clinic of Trauma and Reconstructive Surgery, University Hospital &quot;Carl Gustav Carus&quot;, Dresden, GermanyAbstract: The synthetic material Nanobone&reg; (hydroxyapatite nanocrystallines embedded in a porous silica gel matrix) was examined in vivo using a standardized bone defect model in the ovine tibial metaphysis. A standardized 6 &times; 12 &times; 24-mm bone defect was created below the articular surface of the medial tibia condyles on both hind legs of 18 adult sheep. The defect on the right side was filled with Nanobone&reg;, while the defect on the contralateral side was left empty. The tibial heads of six sheep were analyzed after 6, 12, and 26 weeks each. The histological and radiological analysis of the defect on the control side did not reveal any bone formation after the total of 26 weeks. In contrast, the microcomputed tomography analysis of the defect filled with Nanobone&reg; showed a 55%, 72%, and 74% volume fraction of structures with bone density after 6, 12, and 26 weeks, respectively. Quantitative histomorphological analysis after 6, and 12 weeks revealed an osteoneogenesis of 22%, and 36%, respectively. Hematoxylin and eosin sections demonstrated multinucleated giant cells on the surface of the biomaterial and resorption lacunae, indicating osteoclastic resorptive activity. Nanobone&reg; appears to be a highly potent bone substitute material with osteoconductive properties in a loaded large animal defect model, supporting the potential use of Nanobone&reg; also in humans.Keywords: biocompatibility, bone ingrowth, hydroxyapatite, osseointegration, osteoconductionMittlmeier TVollmar BRammelt SLenz SGerber TIgnatius AStratos ITaschner THelms KHarms CDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 2883-2889 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Mittlmeier T Vollmar B Rammelt S Lenz S Gerber T Ignatius A Stratos I Taschner T Helms K Harms C Osteogenic capacity of nanocrystalline bone cement in a weight-bearing defect at the ovine tibial metaphysis
description	Christoph Harms,1 Kai Helms,1 Tibor Taschner,1 Ioannis Stratos,1 Anita Ignatius,5 Thomas Gerber,2 Solvig Lenz,3 Stefan Rammelt,6 Brigitte Vollmar,4 Thomas Mittlmeier11Department of Trauma and Reconstructive Surgery, 2Department for Materials Research and Nanostructures, Institute for Physics, 3Department of Oral and Maxillofacial Plastic Surgery, 4Institute for Experimental Surgery, University of Rostock, Rostock, 5Institute of Orthopaedic Research and Biomechanics, University of Ulm, Ulm, 6Clinic of Trauma and Reconstructive Surgery, University Hospital &quot;Carl Gustav Carus&quot;, Dresden, GermanyAbstract: The synthetic material Nanobone&reg; (hydroxyapatite nanocrystallines embedded in a porous silica gel matrix) was examined in vivo using a standardized bone defect model in the ovine tibial metaphysis. A standardized 6 &times; 12 &times; 24-mm bone defect was created below the articular surface of the medial tibia condyles on both hind legs of 18 adult sheep. The defect on the right side was filled with Nanobone&reg;, while the defect on the contralateral side was left empty. The tibial heads of six sheep were analyzed after 6, 12, and 26 weeks each. The histological and radiological analysis of the defect on the control side did not reveal any bone formation after the total of 26 weeks. In contrast, the microcomputed tomography analysis of the defect filled with Nanobone&reg; showed a 55%, 72%, and 74% volume fraction of structures with bone density after 6, 12, and 26 weeks, respectively. Quantitative histomorphological analysis after 6, and 12 weeks revealed an osteoneogenesis of 22%, and 36%, respectively. Hematoxylin and eosin sections demonstrated multinucleated giant cells on the surface of the biomaterial and resorption lacunae, indicating osteoclastic resorptive activity. Nanobone&reg; appears to be a highly potent bone substitute material with osteoconductive properties in a loaded large animal defect model, supporting the potential use of Nanobone&reg; also in humans.Keywords: biocompatibility, bone ingrowth, hydroxyapatite, osseointegration, osteoconduction
format	article
author	Mittlmeier T Vollmar B Rammelt S Lenz S Gerber T Ignatius A Stratos I Taschner T Helms K Harms C
author_facet	Mittlmeier T Vollmar B Rammelt S Lenz S Gerber T Ignatius A Stratos I Taschner T Helms K Harms C
author_sort	Mittlmeier T
title	Osteogenic capacity of nanocrystalline bone cement in a weight-bearing defect at the ovine tibial metaphysis
title_short	Osteogenic capacity of nanocrystalline bone cement in a weight-bearing defect at the ovine tibial metaphysis
title_full	Osteogenic capacity of nanocrystalline bone cement in a weight-bearing defect at the ovine tibial metaphysis
title_fullStr	Osteogenic capacity of nanocrystalline bone cement in a weight-bearing defect at the ovine tibial metaphysis
title_full_unstemmed	Osteogenic capacity of nanocrystalline bone cement in a weight-bearing defect at the ovine tibial metaphysis
title_sort	osteogenic capacity of nanocrystalline bone cement in a weight-bearing defect at the ovine tibial metaphysis
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/8ab9c875a2134d438adf096231026eb3
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Osteogenic capacity of nanocrystalline bone cement in a weight-bearing defect at the ovine tibial metaphysis

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