Prognosis of severe cytomegalovirus infection in newborns

Objective is to study the features of impaired activation of T and B lymphocytes in order to predicting severe cytomegalovirus infection in newborns. Materials and methods. 133 newborns with cytomegalovirus infection were examined. Immediately after diagnosing cytomegalovirus infection, all patients...

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Autor principal:	L. V. Kravchenko
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2021
Materias:	neonates cytomegalovirus infection t lymphocytes impaired activation Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/8aba3931042745eca82df25e808cf67c
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spelling	oai:doaj.org-article:8aba3931042745eca82df25e808cf67c2021-11-22T07:09:55ZPrognosis of severe cytomegalovirus infection in newborns2220-76192313-739810.15789/2220-7619-POS-1537https://doaj.org/article/8aba3931042745eca82df25e808cf67c2021-09-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/1537https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Objective is to study the features of impaired activation of T and B lymphocytes in order to predicting severe cytomegalovirus infection in newborns. Materials and methods. 133 newborns with cytomegalovirus infection were examined. Immediately after diagnosing cytomegalovirus infection, all patients observed were immunologically ex amined, including assessing count of peripheral blood T and B lymphocytes, as well as their intercellular interaction by using flow cytometry immunostaining for CD3, CD3+CD28–, CD3+CD28+, CD3–CD28+, CD4, CD8, CD20, CD20+CD40+, CD28, CD40. The test was performed by using a Beckman Coulter Epics XL laser flow cytofluorometer. Depending on the condition severity, all children were divided into two groups: 1 — cytomegalovirus infection, severe form — 60 subjects (45.1%); 2 — cytomegalovirus infection, moderate form — 73 subjects (54.9%). Results of the entire set of studied indicators for cellular and humoral arms of immune system revealed statistically significant differences for the prognosis of severe cytomegalovirus infection: CD3+CD28–, CD20, CD20+CD40+, CD4. T lymphocytes with CD3+CD28+ activation markers, through which costimulating signals necessary for the activation of T helper cells are exerted cell-intrinsic features, serving as an important factor ensuring immune response. Using the “classification trees” method, we developed a differentiated approach to forecast severe cytomegalovirus infection in newborns. Systems of inequalities were obtained, four of which classify a subgroup of newborns with severe cytomegalovirus infection. The consistent application of the obtained inequalities makes it possible to isolate from the input stream of sick patients with a prognosis of the development of severe cytomegalovirus infection. The proposed diagnostic rules can be considered as screening markers for predicting a severe cytomegalovirus infection in newborns, which makes possible the timely onset of specific therapy.L. V. KravchenkoSankt-Peterburg : NIIÈM imeni Pasteraarticleneonatescytomegalovirus infectiont lymphocytesimpaired activationInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 11, Iss 4, Pp 745-751 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	neonates cytomegalovirus infection t lymphocytes impaired activation Infectious and parasitic diseases RC109-216
spellingShingle	neonates cytomegalovirus infection t lymphocytes impaired activation Infectious and parasitic diseases RC109-216 L. V. Kravchenko Prognosis of severe cytomegalovirus infection in newborns
description	Objective is to study the features of impaired activation of T and B lymphocytes in order to predicting severe cytomegalovirus infection in newborns. Materials and methods. 133 newborns with cytomegalovirus infection were examined. Immediately after diagnosing cytomegalovirus infection, all patients observed were immunologically ex amined, including assessing count of peripheral blood T and B lymphocytes, as well as their intercellular interaction by using flow cytometry immunostaining for CD3, CD3+CD28–, CD3+CD28+, CD3–CD28+, CD4, CD8, CD20, CD20+CD40+, CD28, CD40. The test was performed by using a Beckman Coulter Epics XL laser flow cytofluorometer. Depending on the condition severity, all children were divided into two groups: 1 — cytomegalovirus infection, severe form — 60 subjects (45.1%); 2 — cytomegalovirus infection, moderate form — 73 subjects (54.9%). Results of the entire set of studied indicators for cellular and humoral arms of immune system revealed statistically significant differences for the prognosis of severe cytomegalovirus infection: CD3+CD28–, CD20, CD20+CD40+, CD4. T lymphocytes with CD3+CD28+ activation markers, through which costimulating signals necessary for the activation of T helper cells are exerted cell-intrinsic features, serving as an important factor ensuring immune response. Using the “classification trees” method, we developed a differentiated approach to forecast severe cytomegalovirus infection in newborns. Systems of inequalities were obtained, four of which classify a subgroup of newborns with severe cytomegalovirus infection. The consistent application of the obtained inequalities makes it possible to isolate from the input stream of sick patients with a prognosis of the development of severe cytomegalovirus infection. The proposed diagnostic rules can be considered as screening markers for predicting a severe cytomegalovirus infection in newborns, which makes possible the timely onset of specific therapy.
format	article
author	L. V. Kravchenko
author_facet	L. V. Kravchenko
author_sort	L. V. Kravchenko
title	Prognosis of severe cytomegalovirus infection in newborns
title_short	Prognosis of severe cytomegalovirus infection in newborns
title_full	Prognosis of severe cytomegalovirus infection in newborns
title_fullStr	Prognosis of severe cytomegalovirus infection in newborns
title_full_unstemmed	Prognosis of severe cytomegalovirus infection in newborns
title_sort	prognosis of severe cytomegalovirus infection in newborns
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2021
url	https://doaj.org/article/8aba3931042745eca82df25e808cf67c
work_keys_str_mv	AT lvkravchenko prognosisofseverecytomegalovirusinfectioninnewborns
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Prognosis of severe cytomegalovirus infection in newborns

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