Parasitoid wasp venom vesicles (venosomes) enter Drosophila melanogaster lamellocytes through a flotillin/lipid raft-dependent endocytic pathway

Venosomes are extracellular vesicles found in the venom of Leptopilina endoparasitoids wasps, which transport and target virulence factors to impair the parasitoid egg encapsulation by the lamellocytes of their Drosophila melanogaster host larva. Using the co-immunolocalization of fluorescent L. bou...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bin Wan, Marylène Poirié, Jean-Luc Gatti
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	lamellocytes endosomes lysosomes rafts drosophila melanogaster venosomes parasitoid wasps confocal microscopy Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/8abb264d027f459fa9ed5ba8e4e9106c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8abb264d027f459fa9ed5ba8e4e9106c
record_format	dspace
spelling	oai:doaj.org-article:8abb264d027f459fa9ed5ba8e4e9106c2021-11-17T14:21:59ZParasitoid wasp venom vesicles (venosomes) enter Drosophila melanogaster lamellocytes through a flotillin/lipid raft-dependent endocytic pathway2150-55942150-560810.1080/21505594.2020.1838116https://doaj.org/article/8abb264d027f459fa9ed5ba8e4e9106c2020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1838116https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Venosomes are extracellular vesicles found in the venom of Leptopilina endoparasitoids wasps, which transport and target virulence factors to impair the parasitoid egg encapsulation by the lamellocytes of their Drosophila melanogaster host larva. Using the co-immunolocalization of fluorescent L. boulardi venosomes and one of the putative-transported virulence factors, LbGAP, with known markers of cellular endocytosis, we show that venosomes endocytosis by lamellocytes is not a process dependent on clathrin or macropinocytosis and internalization seems to bypass the early endosomal compartment Rab5. After internalization, LbGAP colocalizes strongly with flotillin-1 and the GPI-anchored protein Atilla/L1 (a lamellocyte surface marker) suggesting that entry occurs via a flotillin/lipid raft-dependent pathway. Once internalized, venosomes reach all intracellular compartments, including late and recycling endosomes, lysosomes, and the endoplasmic reticulum network. Venosomes therefore enter their target cells by a specific mechanism and the virulence factors are widely distributed in the lamellocytes’ compartments to impair their functions.Bin WanMarylène PoiriéJean-Luc GattiTaylor & Francis Grouparticlelamellocytesendosomeslysosomesraftsdrosophila melanogastervenosomesparasitoid waspsconfocal microscopyInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 1512-1521 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	lamellocytes endosomes lysosomes rafts drosophila melanogaster venosomes parasitoid wasps confocal microscopy Infectious and parasitic diseases RC109-216
spellingShingle	lamellocytes endosomes lysosomes rafts drosophila melanogaster venosomes parasitoid wasps confocal microscopy Infectious and parasitic diseases RC109-216 Bin Wan Marylène Poirié Jean-Luc Gatti Parasitoid wasp venom vesicles (venosomes) enter Drosophila melanogaster lamellocytes through a flotillin/lipid raft-dependent endocytic pathway
description	Venosomes are extracellular vesicles found in the venom of Leptopilina endoparasitoids wasps, which transport and target virulence factors to impair the parasitoid egg encapsulation by the lamellocytes of their Drosophila melanogaster host larva. Using the co-immunolocalization of fluorescent L. boulardi venosomes and one of the putative-transported virulence factors, LbGAP, with known markers of cellular endocytosis, we show that venosomes endocytosis by lamellocytes is not a process dependent on clathrin or macropinocytosis and internalization seems to bypass the early endosomal compartment Rab5. After internalization, LbGAP colocalizes strongly with flotillin-1 and the GPI-anchored protein Atilla/L1 (a lamellocyte surface marker) suggesting that entry occurs via a flotillin/lipid raft-dependent pathway. Once internalized, venosomes reach all intracellular compartments, including late and recycling endosomes, lysosomes, and the endoplasmic reticulum network. Venosomes therefore enter their target cells by a specific mechanism and the virulence factors are widely distributed in the lamellocytes’ compartments to impair their functions.
format	article
author	Bin Wan Marylène Poirié Jean-Luc Gatti
author_facet	Bin Wan Marylène Poirié Jean-Luc Gatti
author_sort	Bin Wan
title	Parasitoid wasp venom vesicles (venosomes) enter Drosophila melanogaster lamellocytes through a flotillin/lipid raft-dependent endocytic pathway
title_short	Parasitoid wasp venom vesicles (venosomes) enter Drosophila melanogaster lamellocytes through a flotillin/lipid raft-dependent endocytic pathway
title_full	Parasitoid wasp venom vesicles (venosomes) enter Drosophila melanogaster lamellocytes through a flotillin/lipid raft-dependent endocytic pathway
title_fullStr	Parasitoid wasp venom vesicles (venosomes) enter Drosophila melanogaster lamellocytes through a flotillin/lipid raft-dependent endocytic pathway
title_full_unstemmed	Parasitoid wasp venom vesicles (venosomes) enter Drosophila melanogaster lamellocytes through a flotillin/lipid raft-dependent endocytic pathway
title_sort	parasitoid wasp venom vesicles (venosomes) enter drosophila melanogaster lamellocytes through a flotillin/lipid raft-dependent endocytic pathway
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/8abb264d027f459fa9ed5ba8e4e9106c
work_keys_str_mv	AT binwan parasitoidwaspvenomvesiclesvenosomesenterdrosophilamelanogasterlamellocytesthroughaflotillinlipidraftdependentendocyticpathway AT marylenepoirie parasitoidwaspvenomvesiclesvenosomesenterdrosophilamelanogasterlamellocytesthroughaflotillinlipidraftdependentendocyticpathway AT jeanlucgatti parasitoidwaspvenomvesiclesvenosomesenterdrosophilamelanogasterlamellocytesthroughaflotillinlipidraftdependentendocyticpathway
_version_	1718425427693797376

Parasitoid wasp venom vesicles (venosomes) enter Drosophila melanogaster lamellocytes through a flotillin/lipid raft-dependent endocytic pathway

Ejemplares similares