Low-intensity pulsed ultrasound therapy suppresses coronary adventitial inflammatory changes and hyperconstricting responses after coronary stent implantation in pigs in vivo.

<h4>Backgrounds</h4>We demonstrated that coronary adventitial inflammation plays important roles in the pathogenesis of drug-eluting stent (DES)-induced coronary hyperconstricting responses in pigs in vivo. However, no therapy is yet available to treat coronary adventitial inflammation....

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Autores principales:	Tasuku Watanabe, Yasuharu Matsumoto, Kensuke Nishimiya, Tomohiko Shindo, Hirokazu Amamizu, Jun Sugisawa, Satoshi Tsuchiya, Koichi Sato, Susumu Morosawa, Kazuma Ohyama, Tomomi Watanabe-Asaka, Moyuru Hayashi, Yoshiko Kawai, Jun Takahashi, Satoshi Yasuda, Hiroaki Shimokawa
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8abb7f777a514b419ae798fe61081292
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spelling	oai:doaj.org-article:8abb7f777a514b419ae798fe610812922021-12-02T20:08:17ZLow-intensity pulsed ultrasound therapy suppresses coronary adventitial inflammatory changes and hyperconstricting responses after coronary stent implantation in pigs in vivo.1932-620310.1371/journal.pone.0257175https://doaj.org/article/8abb7f777a514b419ae798fe610812922021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257175https://doaj.org/toc/1932-6203<h4>Backgrounds</h4>We demonstrated that coronary adventitial inflammation plays important roles in the pathogenesis of drug-eluting stent (DES)-induced coronary hyperconstricting responses in pigs in vivo. However, no therapy is yet available to treat coronary adventitial inflammation. We thus developed the low-intensity pulsed ultrasound (LIPUS) therapy that ameliorates myocardial ischemia by enhancing angiogenesis.<h4>Aims</h4>We aimed to examine whether our LIPUS therapy suppresses DES-induced coronary hyperconstricting responses in pigs in vivo, and if so, what mechanisms are involved.<h4>Methods</h4>Sixteen normal male pigs were randomly assigned to the LIPUS or the sham therapy groups after DES implantation into the left anterior descending (LAD) coronary artery. In the LIPUS group, LIPUS (32 cycles, 193 mW/cm2) was applied to the heart at 3 different levels (segments proximal and distal to the stent edges and middle of the stent) for 20 min at each level for every other day for 2 weeks. The sham therapy group was treated in the same manner but without LIPUS. At 4 weeks after stent implantation, we performed coronary angiography, followed by immunohistological analysis.<h4>Results</h4>Coronary vasoconstricting responses to serotonin in LAD at DES edges were significantly suppressed in the LIPUS group compared with the sham group. Furthermore, lymph transport speed in vivo was significantly faster in the LIPUS group than in the sham group. Histological analysis at DES edges showed that inflammatory changes and Rho-kinase activity were significantly suppressed in the LIPUS group, associated with eNOS up-regulation and enhanced lymph-angiogenesis.<h4>Conclusions</h4>These results suggest that our non-invasive LIPUS therapy is useful to treat coronary functional abnormalities caused by coronary adventitial inflammation, indicating its potential for the novel and safe therapeutic approach of coronary artery disease.Tasuku WatanabeYasuharu MatsumotoKensuke NishimiyaTomohiko ShindoHirokazu AmamizuJun SugisawaSatoshi TsuchiyaKoichi SatoSusumu MorosawaKazuma OhyamaTomomi Watanabe-AsakaMoyuru HayashiYoshiko KawaiJun TakahashiSatoshi YasudaHiroaki ShimokawaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0257175 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tasuku Watanabe Yasuharu Matsumoto Kensuke Nishimiya Tomohiko Shindo Hirokazu Amamizu Jun Sugisawa Satoshi Tsuchiya Koichi Sato Susumu Morosawa Kazuma Ohyama Tomomi Watanabe-Asaka Moyuru Hayashi Yoshiko Kawai Jun Takahashi Satoshi Yasuda Hiroaki Shimokawa Low-intensity pulsed ultrasound therapy suppresses coronary adventitial inflammatory changes and hyperconstricting responses after coronary stent implantation in pigs in vivo.
description	<h4>Backgrounds</h4>We demonstrated that coronary adventitial inflammation plays important roles in the pathogenesis of drug-eluting stent (DES)-induced coronary hyperconstricting responses in pigs in vivo. However, no therapy is yet available to treat coronary adventitial inflammation. We thus developed the low-intensity pulsed ultrasound (LIPUS) therapy that ameliorates myocardial ischemia by enhancing angiogenesis.<h4>Aims</h4>We aimed to examine whether our LIPUS therapy suppresses DES-induced coronary hyperconstricting responses in pigs in vivo, and if so, what mechanisms are involved.<h4>Methods</h4>Sixteen normal male pigs were randomly assigned to the LIPUS or the sham therapy groups after DES implantation into the left anterior descending (LAD) coronary artery. In the LIPUS group, LIPUS (32 cycles, 193 mW/cm2) was applied to the heart at 3 different levels (segments proximal and distal to the stent edges and middle of the stent) for 20 min at each level for every other day for 2 weeks. The sham therapy group was treated in the same manner but without LIPUS. At 4 weeks after stent implantation, we performed coronary angiography, followed by immunohistological analysis.<h4>Results</h4>Coronary vasoconstricting responses to serotonin in LAD at DES edges were significantly suppressed in the LIPUS group compared with the sham group. Furthermore, lymph transport speed in vivo was significantly faster in the LIPUS group than in the sham group. Histological analysis at DES edges showed that inflammatory changes and Rho-kinase activity were significantly suppressed in the LIPUS group, associated with eNOS up-regulation and enhanced lymph-angiogenesis.<h4>Conclusions</h4>These results suggest that our non-invasive LIPUS therapy is useful to treat coronary functional abnormalities caused by coronary adventitial inflammation, indicating its potential for the novel and safe therapeutic approach of coronary artery disease.
format	article
author	Tasuku Watanabe Yasuharu Matsumoto Kensuke Nishimiya Tomohiko Shindo Hirokazu Amamizu Jun Sugisawa Satoshi Tsuchiya Koichi Sato Susumu Morosawa Kazuma Ohyama Tomomi Watanabe-Asaka Moyuru Hayashi Yoshiko Kawai Jun Takahashi Satoshi Yasuda Hiroaki Shimokawa
author_facet	Tasuku Watanabe Yasuharu Matsumoto Kensuke Nishimiya Tomohiko Shindo Hirokazu Amamizu Jun Sugisawa Satoshi Tsuchiya Koichi Sato Susumu Morosawa Kazuma Ohyama Tomomi Watanabe-Asaka Moyuru Hayashi Yoshiko Kawai Jun Takahashi Satoshi Yasuda Hiroaki Shimokawa
author_sort	Tasuku Watanabe
title	Low-intensity pulsed ultrasound therapy suppresses coronary adventitial inflammatory changes and hyperconstricting responses after coronary stent implantation in pigs in vivo.
title_short	Low-intensity pulsed ultrasound therapy suppresses coronary adventitial inflammatory changes and hyperconstricting responses after coronary stent implantation in pigs in vivo.
title_full	Low-intensity pulsed ultrasound therapy suppresses coronary adventitial inflammatory changes and hyperconstricting responses after coronary stent implantation in pigs in vivo.
title_fullStr	Low-intensity pulsed ultrasound therapy suppresses coronary adventitial inflammatory changes and hyperconstricting responses after coronary stent implantation in pigs in vivo.
title_full_unstemmed	Low-intensity pulsed ultrasound therapy suppresses coronary adventitial inflammatory changes and hyperconstricting responses after coronary stent implantation in pigs in vivo.
title_sort	low-intensity pulsed ultrasound therapy suppresses coronary adventitial inflammatory changes and hyperconstricting responses after coronary stent implantation in pigs in vivo.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/8abb7f777a514b419ae798fe61081292
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Low-intensity pulsed ultrasound therapy suppresses coronary adventitial inflammatory changes and hyperconstricting responses after coronary stent implantation in pigs in vivo.

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