Establishment of a Novel and Efficient Agrobacterium-Mediated in Planta Transformation System for Passion Fruit (Passiflora edulis)

Passion fruit (Passiflora edulis) is an important fruit crop with high economic value. Genetic engineering plays an important role in crop improvement with desired traits and gene functional studies. The lack of a simple, efficient, and stable transformation system for passion fru...

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Autores principales:	Hafiz Muhammad Rizwan, Qiang Yang, Ahmed Fathy Yousef, Xiaoxue Zhang, Yasir Sharif, Jia Kaijie, Meng Shi, Han Li, Nigarish Munir, Xuelian Yang, Xiaoxia Wei, Ralf Oelmüller, Chunzhen Cheng, Faxing Chen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Passiflora edulis</i> dark incubation parafilm wrapping <i>in planta</i> <i>Agrobacterium</i>-mediated transformation Botany QK1-989
Acceso en línea:	https://doaj.org/article/8abe206488404b2fb958f2311aee7cf0
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spelling	oai:doaj.org-article:8abe206488404b2fb958f2311aee7cf02021-11-25T18:46:45ZEstablishment of a Novel and Efficient <i>Agrobacterium</i>-Mediated <i>in Planta</i> Transformation System for Passion Fruit (<i>Passiflora edulis</i>)10.3390/plants101124592223-7747https://doaj.org/article/8abe206488404b2fb958f2311aee7cf02021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2459https://doaj.org/toc/2223-7747Passion fruit (<i>Passiflora edulis</i>) is an important fruit crop with high economic value. Genetic engineering plays an important role in crop improvement with desired traits and gene functional studies. The lack of a simple, efficient, and stable transformation system for passion fruit has greatly limited gene functional studies. In this study, a simple and efficient <i>Agrobacterium</i>-mediated <i>in planta</i> transformation system for passion fruit was established, using <i>Agrobacterium</i> virulent strain EHA105 harboring the binary vectors pCAMBIA1301 and pCAMBIA1302 with GUS and GFP reporter genes. The system requires less time and labor costs than conventional transformation systems, and no additional phytohormones and sterile conditions are required. Regeneration efficiency of 86% and transformation efficiency of 29% were achieved, when the wounds were wrapped with Parafilm and the plants were kept in darkness for 15 days. Approximately 75% of the regenerated plants had a single shoot and 26% multiple shoots. The transformation was confirmed at the DNA and RNA levels as well as by GUS staining and GFP fluorescent measurements. The developed protocol will contribute to the genetic improvement of passion fruit breeding.Hafiz Muhammad RizwanQiang YangAhmed Fathy YousefXiaoxue ZhangYasir SharifJia KaijieMeng ShiHan LiNigarish MunirXuelian YangXiaoxia WeiRalf OelmüllerChunzhen ChengFaxing ChenMDPI AGarticle<i>Passiflora edulis</i>dark incubationparafilm wrapping<i>in planta</i><i>Agrobacterium</i>-mediated transformationBotanyQK1-989ENPlants, Vol 10, Iss 2459, p 2459 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Passiflora edulis</i> dark incubation parafilm wrapping <i>in planta</i> <i>Agrobacterium</i>-mediated transformation Botany QK1-989
spellingShingle	<i>Passiflora edulis</i> dark incubation parafilm wrapping <i>in planta</i> <i>Agrobacterium</i>-mediated transformation Botany QK1-989 Hafiz Muhammad Rizwan Qiang Yang Ahmed Fathy Yousef Xiaoxue Zhang Yasir Sharif Jia Kaijie Meng Shi Han Li Nigarish Munir Xuelian Yang Xiaoxia Wei Ralf Oelmüller Chunzhen Cheng Faxing Chen Establishment of a Novel and Efficient <i>Agrobacterium</i>-Mediated <i>in Planta</i> Transformation System for Passion Fruit (<i>Passiflora edulis</i>)
description	Passion fruit (<i>Passiflora edulis</i>) is an important fruit crop with high economic value. Genetic engineering plays an important role in crop improvement with desired traits and gene functional studies. The lack of a simple, efficient, and stable transformation system for passion fruit has greatly limited gene functional studies. In this study, a simple and efficient <i>Agrobacterium</i>-mediated <i>in planta</i> transformation system for passion fruit was established, using <i>Agrobacterium</i> virulent strain EHA105 harboring the binary vectors pCAMBIA1301 and pCAMBIA1302 with GUS and GFP reporter genes. The system requires less time and labor costs than conventional transformation systems, and no additional phytohormones and sterile conditions are required. Regeneration efficiency of 86% and transformation efficiency of 29% were achieved, when the wounds were wrapped with Parafilm and the plants were kept in darkness for 15 days. Approximately 75% of the regenerated plants had a single shoot and 26% multiple shoots. The transformation was confirmed at the DNA and RNA levels as well as by GUS staining and GFP fluorescent measurements. The developed protocol will contribute to the genetic improvement of passion fruit breeding.
format	article
author	Hafiz Muhammad Rizwan Qiang Yang Ahmed Fathy Yousef Xiaoxue Zhang Yasir Sharif Jia Kaijie Meng Shi Han Li Nigarish Munir Xuelian Yang Xiaoxia Wei Ralf Oelmüller Chunzhen Cheng Faxing Chen
author_facet	Hafiz Muhammad Rizwan Qiang Yang Ahmed Fathy Yousef Xiaoxue Zhang Yasir Sharif Jia Kaijie Meng Shi Han Li Nigarish Munir Xuelian Yang Xiaoxia Wei Ralf Oelmüller Chunzhen Cheng Faxing Chen
author_sort	Hafiz Muhammad Rizwan
title	Establishment of a Novel and Efficient <i>Agrobacterium</i>-Mediated <i>in Planta</i> Transformation System for Passion Fruit (<i>Passiflora edulis</i>)
title_short	Establishment of a Novel and Efficient <i>Agrobacterium</i>-Mediated <i>in Planta</i> Transformation System for Passion Fruit (<i>Passiflora edulis</i>)
title_full	Establishment of a Novel and Efficient <i>Agrobacterium</i>-Mediated <i>in Planta</i> Transformation System for Passion Fruit (<i>Passiflora edulis</i>)
title_fullStr	Establishment of a Novel and Efficient <i>Agrobacterium</i>-Mediated <i>in Planta</i> Transformation System for Passion Fruit (<i>Passiflora edulis</i>)
title_full_unstemmed	Establishment of a Novel and Efficient <i>Agrobacterium</i>-Mediated <i>in Planta</i> Transformation System for Passion Fruit (<i>Passiflora edulis</i>)
title_sort	establishment of a novel and efficient <i>agrobacterium</i>-mediated <i>in planta</i> transformation system for passion fruit (<i>passiflora edulis</i>)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8abe206488404b2fb958f2311aee7cf0
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Establishment of a Novel and Efficient <i>Agrobacterium</i>-Mediated <i>in Planta</i> Transformation System for Passion Fruit (<i>Passiflora edulis</i>)

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