Characterization of GMPP from Dendrobium huoshanense yielding GDP-D-mannose

Dendrobium huoshanense has been used for centuries in China and its polysaccharides are the main active components in treating loss of body fluids resulting from fever and asthenic symptoms. However, the biosynthetic pathway of polysaccharides in D. huoshanense remains to be elucidated. In this stud...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yi Yuqi, Liu Lulu, Zhou Wenyan, Peng Daiyin, Han Rongchun, Yu Nianjun
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	gdp-mannose pyrophosphorylase polysaccharide biosynthesis guanosine-5-triphosphoric acid Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8abe55b1c8624feaa63a18f3e369bffd
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8abe55b1c8624feaa63a18f3e369bffd
record_format	dspace
spelling	oai:doaj.org-article:8abe55b1c8624feaa63a18f3e369bffd2021-12-05T14:10:40ZCharacterization of GMPP from Dendrobium huoshanense yielding GDP-D-mannose2391-541210.1515/biol-2021-0015https://doaj.org/article/8abe55b1c8624feaa63a18f3e369bffd2021-02-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0015https://doaj.org/toc/2391-5412Dendrobium huoshanense has been used for centuries in China and its polysaccharides are the main active components in treating loss of body fluids resulting from fever and asthenic symptoms. However, the biosynthetic pathway of polysaccharides in D. huoshanense remains to be elucidated. In this study, we obtained a guanosine diphosphate (GDP)-mannose pyrophosphorylase (DhGMPP) from D. huoshanense and characterized its function to catalyze the conversion of α-D-mannose-phosphate to GDP-D-mannose involved in the production of polysaccharides. DhGMPP, with the open reading frame of 1,245 bp, was isolated from RNA-Seq data of D. huoshanense. Phylogenetic analysis as well as sequence characterization suggested its involvement in the biosynthesis of GDP-D-mannose. In vitro enzyme assay demonstrated that GMPP encoded a pyrophosphorylase that converted α-D-mannose-phosphate and GTP into GDP-D-mannose. Identification of DhGMPP could provide more insights into the mechanism concerning polysaccharide biosynthesis in D. huoshanense and be utilized for enhancing polysaccharide accumulation through metabolic engineering.Yi YuqiLiu LuluZhou WenyanPeng DaiyinHan RongchunYu NianjunDe Gruyterarticlegdp-mannose pyrophosphorylasepolysaccharide biosynthesisguanosine-5-triphosphoric acidBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 102-107 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gdp-mannose pyrophosphorylase polysaccharide biosynthesis guanosine-5-triphosphoric acid Biology (General) QH301-705.5
spellingShingle	gdp-mannose pyrophosphorylase polysaccharide biosynthesis guanosine-5-triphosphoric acid Biology (General) QH301-705.5 Yi Yuqi Liu Lulu Zhou Wenyan Peng Daiyin Han Rongchun Yu Nianjun Characterization of GMPP from Dendrobium huoshanense yielding GDP-D-mannose
description	Dendrobium huoshanense has been used for centuries in China and its polysaccharides are the main active components in treating loss of body fluids resulting from fever and asthenic symptoms. However, the biosynthetic pathway of polysaccharides in D. huoshanense remains to be elucidated. In this study, we obtained a guanosine diphosphate (GDP)-mannose pyrophosphorylase (DhGMPP) from D. huoshanense and characterized its function to catalyze the conversion of α-D-mannose-phosphate to GDP-D-mannose involved in the production of polysaccharides. DhGMPP, with the open reading frame of 1,245 bp, was isolated from RNA-Seq data of D. huoshanense. Phylogenetic analysis as well as sequence characterization suggested its involvement in the biosynthesis of GDP-D-mannose. In vitro enzyme assay demonstrated that GMPP encoded a pyrophosphorylase that converted α-D-mannose-phosphate and GTP into GDP-D-mannose. Identification of DhGMPP could provide more insights into the mechanism concerning polysaccharide biosynthesis in D. huoshanense and be utilized for enhancing polysaccharide accumulation through metabolic engineering.
format	article
author	Yi Yuqi Liu Lulu Zhou Wenyan Peng Daiyin Han Rongchun Yu Nianjun
author_facet	Yi Yuqi Liu Lulu Zhou Wenyan Peng Daiyin Han Rongchun Yu Nianjun
author_sort	Yi Yuqi
title	Characterization of GMPP from Dendrobium huoshanense yielding GDP-D-mannose
title_short	Characterization of GMPP from Dendrobium huoshanense yielding GDP-D-mannose
title_full	Characterization of GMPP from Dendrobium huoshanense yielding GDP-D-mannose
title_fullStr	Characterization of GMPP from Dendrobium huoshanense yielding GDP-D-mannose
title_full_unstemmed	Characterization of GMPP from Dendrobium huoshanense yielding GDP-D-mannose
title_sort	characterization of gmpp from dendrobium huoshanense yielding gdp-d-mannose
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/8abe55b1c8624feaa63a18f3e369bffd
work_keys_str_mv	AT yiyuqi characterizationofgmppfromdendrobiumhuoshanenseyieldinggdpdmannose AT liululu characterizationofgmppfromdendrobiumhuoshanenseyieldinggdpdmannose AT zhouwenyan characterizationofgmppfromdendrobiumhuoshanenseyieldinggdpdmannose AT pengdaiyin characterizationofgmppfromdendrobiumhuoshanenseyieldinggdpdmannose AT hanrongchun characterizationofgmppfromdendrobiumhuoshanenseyieldinggdpdmannose AT yunianjun characterizationofgmppfromdendrobiumhuoshanenseyieldinggdpdmannose
_version_	1718371850730340352

Characterization of GMPP from Dendrobium huoshanense yielding GDP-D-mannose

Ejemplares similares