Biodegradation of Crude Oil Spill Using Bacillus subtilis and Pseudomonas putida in Sequencing Method

Crude oil, otherwise called petroleum, occurs naturally as a complex organic mixture underneath the subsurface. The activities related to its exploration, production, refining, storage and distribution are mostly accompanied with extreme pollution and other hazardous conditions. For these reasons, t...

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Autores principales:	Harmin Sulistiyaning Titah, Herman Pratikno, Ipung Fitri Purwanti, Widhowati Kesoema Wardhani
Formato:	article
Lenguaje:	EN
Publicado:	Polish Society of Ecological Engineering (PTIE) 2021
Materias:	biodegradation tph crude oil seawater sequence bacteria inoculum single bacteria inoculum Environmental technology. Sanitary engineering TD1-1066 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/8ac7a1c18003413ba238c2235a83e891
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spelling	oai:doaj.org-article:8ac7a1c18003413ba238c2235a83e8912021-11-04T08:03:23ZBiodegradation of Crude Oil Spill Using Bacillus subtilis and Pseudomonas putida in Sequencing Method2299-899310.12911/22998993/142913https://doaj.org/article/8ac7a1c18003413ba238c2235a83e8912021-12-01T00:00:00Zhttp://www.jeeng.net/Biodegradation-of-Crude-Oil-Spill-Using-Bacillus-subtilis-and-Pseudomonas-putida,142913,0,2.htmlhttps://doaj.org/toc/2299-8993Crude oil, otherwise called petroleum, occurs naturally as a complex organic mixture underneath the subsurface. The activities related to its exploration, production, refining, storage and distribution are mostly accompanied with extreme pollution and other hazardous conditions. For these reasons, the need to critically devise the best possible solutions becomes paramount, particularly as regards oil spills. Therefore, the purpose of this research was to determine the efficiency of TPH removal in crude oil using Bacillus Subtilis and Pseudomonas Putida. The sequencing method was applied in a laboratory scale and under artificial seawater media conditions. The total petroleum hydrocarbon (TPH) serves as a significant parameter in detecting crude oil, although the extraction and analysis were conducted with the use of a separator funnel and gas chromatography mass spectrometry (GCMS), respectively. In addition, the simulated seawater media was described as the mineral salt medium (MSM), with 33% salinity. Moreover, five reactors were also employed, including K for control, B for B. subtilis, P for P. putida, BP for B. subtilis and P. putida sequence and PB for P. putida and B. subtilis sequence. The entire treatments obtained the access to two replicate reactors. Furthermore, the bacteria inoculum and crude oil concentration in each unit were estimated at 5% and 10% (v/v), respectively. The results achieved the maximum TPH removal at 66.29% in the PB reactor after 35 days. On the basis of ANOVA reports, no significant variation was observed between the sequential additions of a single bacterial treatment and consortium microbes. In summary, two bacterial species demonstrated high potential to degrade TPH, but predicted an increase in the break down time, as the nutrient or oxygen tends to accelerate the process.Harmin Sulistiyaning TitahHerman PratiknoIpung Fitri PurwantiWidhowati Kesoema WardhaniPolish Society of Ecological Engineering (PTIE)articlebiodegradationtphcrude oilseawatersequence bacteria inoculumsingle bacteria inoculumEnvironmental technology. Sanitary engineeringTD1-1066Environmental sciencesGE1-350ENJournal of Ecological Engineering, Vol 22, Iss 11, Pp 157-167 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biodegradation tph crude oil seawater sequence bacteria inoculum single bacteria inoculum Environmental technology. Sanitary engineering TD1-1066 Environmental sciences GE1-350
spellingShingle	biodegradation tph crude oil seawater sequence bacteria inoculum single bacteria inoculum Environmental technology. Sanitary engineering TD1-1066 Environmental sciences GE1-350 Harmin Sulistiyaning Titah Herman Pratikno Ipung Fitri Purwanti Widhowati Kesoema Wardhani Biodegradation of Crude Oil Spill Using Bacillus subtilis and Pseudomonas putida in Sequencing Method
description	Crude oil, otherwise called petroleum, occurs naturally as a complex organic mixture underneath the subsurface. The activities related to its exploration, production, refining, storage and distribution are mostly accompanied with extreme pollution and other hazardous conditions. For these reasons, the need to critically devise the best possible solutions becomes paramount, particularly as regards oil spills. Therefore, the purpose of this research was to determine the efficiency of TPH removal in crude oil using Bacillus Subtilis and Pseudomonas Putida. The sequencing method was applied in a laboratory scale and under artificial seawater media conditions. The total petroleum hydrocarbon (TPH) serves as a significant parameter in detecting crude oil, although the extraction and analysis were conducted with the use of a separator funnel and gas chromatography mass spectrometry (GCMS), respectively. In addition, the simulated seawater media was described as the mineral salt medium (MSM), with 33% salinity. Moreover, five reactors were also employed, including K for control, B for B. subtilis, P for P. putida, BP for B. subtilis and P. putida sequence and PB for P. putida and B. subtilis sequence. The entire treatments obtained the access to two replicate reactors. Furthermore, the bacteria inoculum and crude oil concentration in each unit were estimated at 5% and 10% (v/v), respectively. The results achieved the maximum TPH removal at 66.29% in the PB reactor after 35 days. On the basis of ANOVA reports, no significant variation was observed between the sequential additions of a single bacterial treatment and consortium microbes. In summary, two bacterial species demonstrated high potential to degrade TPH, but predicted an increase in the break down time, as the nutrient or oxygen tends to accelerate the process.
format	article
author	Harmin Sulistiyaning Titah Herman Pratikno Ipung Fitri Purwanti Widhowati Kesoema Wardhani
author_facet	Harmin Sulistiyaning Titah Herman Pratikno Ipung Fitri Purwanti Widhowati Kesoema Wardhani
author_sort	Harmin Sulistiyaning Titah
title	Biodegradation of Crude Oil Spill Using Bacillus subtilis and Pseudomonas putida in Sequencing Method
title_short	Biodegradation of Crude Oil Spill Using Bacillus subtilis and Pseudomonas putida in Sequencing Method
title_full	Biodegradation of Crude Oil Spill Using Bacillus subtilis and Pseudomonas putida in Sequencing Method
title_fullStr	Biodegradation of Crude Oil Spill Using Bacillus subtilis and Pseudomonas putida in Sequencing Method
title_full_unstemmed	Biodegradation of Crude Oil Spill Using Bacillus subtilis and Pseudomonas putida in Sequencing Method
title_sort	biodegradation of crude oil spill using bacillus subtilis and pseudomonas putida in sequencing method
publisher	Polish Society of Ecological Engineering (PTIE)
publishDate	2021
url	https://doaj.org/article/8ac7a1c18003413ba238c2235a83e891
work_keys_str_mv	AT harminsulistiyaningtitah biodegradationofcrudeoilspillusingbacillussubtilisandpseudomonasputidainsequencingmethod AT hermanpratikno biodegradationofcrudeoilspillusingbacillussubtilisandpseudomonasputidainsequencingmethod AT ipungfitripurwanti biodegradationofcrudeoilspillusingbacillussubtilisandpseudomonasputidainsequencingmethod AT widhowatikesoemawardhani biodegradationofcrudeoilspillusingbacillussubtilisandpseudomonasputidainsequencingmethod
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Biodegradation of Crude Oil Spill Using Bacillus subtilis and Pseudomonas putida in Sequencing Method

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