Evaluation and diagnostic potential of circulating extracellular vesicle-associated microRNAs in adrenocortical tumors

Abstract There is no available blood marker for the preoperative diagnosis of adrenocortical malignancy. The objective of this study was to investigate the expression of extracellular vesicle-associated microRNAs and their diagnostic potential in plasma samples of patients suffering from adrenocorti...

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Autores principales:	Pál Perge, Henriett Butz, Raffaele Pezzani, Irina Bancos, Zoltán Nagy, Krisztina Pálóczi, Gábor Nyírő, Ábel Decmann, Erna Pap, Michaela Luconi, Massimo Mannelli, Edit I. Buzás, Miklós Tóth, Marco Boscaro, Attila Patócs, Peter Igaz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8ac9e4277c1f449a9d3888d49957b036
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spelling	oai:doaj.org-article:8ac9e4277c1f449a9d3888d49957b0362021-12-02T15:05:44ZEvaluation and diagnostic potential of circulating extracellular vesicle-associated microRNAs in adrenocortical tumors10.1038/s41598-017-05777-02045-2322https://doaj.org/article/8ac9e4277c1f449a9d3888d49957b0362017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05777-0https://doaj.org/toc/2045-2322Abstract There is no available blood marker for the preoperative diagnosis of adrenocortical malignancy. The objective of this study was to investigate the expression of extracellular vesicle-associated microRNAs and their diagnostic potential in plasma samples of patients suffering from adrenocortical tumors. Extracellular vesicles were isolated either by using Total Exosome Isolation Kit or by differential centrifugation/ultracentrifugation. Preoperative plasma extracellular vesicle samples of 6 adrenocortical adenomas (ACA) and 6 histologically verified adrenocortical cancer (ACC) were first screened by Taqman Human Microarray A-cards. Based on the results of screening, two miRNAs were selected and validated by targeted quantitative real-time PCR. The validation cohort included 18 ACAs and 16 ACCs. Beside RNA analysis, extracellular vesicle preparations were also assessed by transmission electron microscopy, flow cytometry and dynamic light scattering. Significant overexpression of hsa-miR-101 and hsa-miR-483-5p in ACC relative to ACA samples has been validated. Receiver operator characteristics of data revealed dCT hsa-miR-483-5p normalized to cel-miR-39 to have the highest diagnostic accuracy (area under curve 0.965), the sensitivity and the specifity were 87.5 and 94.44, respectively. Extracellular vesicle-associated hsa-miR-483-5p thus appears to be a promising minimally invasive biomarker in the preoperative diagnosis of ACC but needs further validation in larger cohorts of patients.Pál PergeHenriett ButzRaffaele PezzaniIrina BancosZoltán NagyKrisztina PálócziGábor NyírőÁbel DecmannErna PapMichaela LuconiMassimo MannelliEdit I. BuzásMiklós TóthMarco BoscaroAttila PatócsPeter IgazNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pál Perge Henriett Butz Raffaele Pezzani Irina Bancos Zoltán Nagy Krisztina Pálóczi Gábor Nyírő Ábel Decmann Erna Pap Michaela Luconi Massimo Mannelli Edit I. Buzás Miklós Tóth Marco Boscaro Attila Patócs Peter Igaz Evaluation and diagnostic potential of circulating extracellular vesicle-associated microRNAs in adrenocortical tumors
description	Abstract There is no available blood marker for the preoperative diagnosis of adrenocortical malignancy. The objective of this study was to investigate the expression of extracellular vesicle-associated microRNAs and their diagnostic potential in plasma samples of patients suffering from adrenocortical tumors. Extracellular vesicles were isolated either by using Total Exosome Isolation Kit or by differential centrifugation/ultracentrifugation. Preoperative plasma extracellular vesicle samples of 6 adrenocortical adenomas (ACA) and 6 histologically verified adrenocortical cancer (ACC) were first screened by Taqman Human Microarray A-cards. Based on the results of screening, two miRNAs were selected and validated by targeted quantitative real-time PCR. The validation cohort included 18 ACAs and 16 ACCs. Beside RNA analysis, extracellular vesicle preparations were also assessed by transmission electron microscopy, flow cytometry and dynamic light scattering. Significant overexpression of hsa-miR-101 and hsa-miR-483-5p in ACC relative to ACA samples has been validated. Receiver operator characteristics of data revealed dCT hsa-miR-483-5p normalized to cel-miR-39 to have the highest diagnostic accuracy (area under curve 0.965), the sensitivity and the specifity were 87.5 and 94.44, respectively. Extracellular vesicle-associated hsa-miR-483-5p thus appears to be a promising minimally invasive biomarker in the preoperative diagnosis of ACC but needs further validation in larger cohorts of patients.
format	article
author	Pál Perge Henriett Butz Raffaele Pezzani Irina Bancos Zoltán Nagy Krisztina Pálóczi Gábor Nyírő Ábel Decmann Erna Pap Michaela Luconi Massimo Mannelli Edit I. Buzás Miklós Tóth Marco Boscaro Attila Patócs Peter Igaz
author_facet	Pál Perge Henriett Butz Raffaele Pezzani Irina Bancos Zoltán Nagy Krisztina Pálóczi Gábor Nyírő Ábel Decmann Erna Pap Michaela Luconi Massimo Mannelli Edit I. Buzás Miklós Tóth Marco Boscaro Attila Patócs Peter Igaz
author_sort	Pál Perge
title	Evaluation and diagnostic potential of circulating extracellular vesicle-associated microRNAs in adrenocortical tumors
title_short	Evaluation and diagnostic potential of circulating extracellular vesicle-associated microRNAs in adrenocortical tumors
title_full	Evaluation and diagnostic potential of circulating extracellular vesicle-associated microRNAs in adrenocortical tumors
title_fullStr	Evaluation and diagnostic potential of circulating extracellular vesicle-associated microRNAs in adrenocortical tumors
title_full_unstemmed	Evaluation and diagnostic potential of circulating extracellular vesicle-associated microRNAs in adrenocortical tumors
title_sort	evaluation and diagnostic potential of circulating extracellular vesicle-associated micrornas in adrenocortical tumors
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8ac9e4277c1f449a9d3888d49957b036
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Evaluation and diagnostic potential of circulating extracellular vesicle-associated microRNAs in adrenocortical tumors

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