Effect on Osteogenic Differentiation of Genetically Modified IL4 or PDGF-BB Over-Expressing and IL4-PDGF-BB Co-Over-Expressing Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro

The use of genetically modified (GM) mesenchymal stromal cells (MSCs) and preconditioned MSCs (pMSCs) may provide further opportunities to improve the outcome of core decompression (CD) for the treatment of early-stage osteonecrosis of the femoral head (ONFH). GM interleukin-4 (IL4) over-expressing...

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Autores principales:	Masanori Tsubosaka, Masahiro Maruyama, Elijah Ejun Huang, Ning Zhang, Takeshi Utsunomiya, Qi Gao, Huaishuang Shen, Xueping Li, Junichi Kushioka, Hirohito Hirata, Zhenyu Yao, Yunzhi Peter Yang, Stuart B. Goodman
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	mesenchymal stromal cells genetically modified preconditioning osteonecrosis core decompression Technology T Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8acc8113415f4067b5fcfc36b60d7aa4
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spelling	oai:doaj.org-article:8acc8113415f4067b5fcfc36b60d7aa42021-11-25T16:46:28ZEffect on Osteogenic Differentiation of Genetically Modified IL4 or PDGF-BB Over-Expressing and IL4-PDGF-BB Co-Over-Expressing Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro10.3390/bioengineering81101652306-5354https://doaj.org/article/8acc8113415f4067b5fcfc36b60d7aa42021-10-01T00:00:00Zhttps://www.mdpi.com/2306-5354/8/11/165https://doaj.org/toc/2306-5354The use of genetically modified (GM) mesenchymal stromal cells (MSCs) and preconditioned MSCs (pMSCs) may provide further opportunities to improve the outcome of core decompression (CD) for the treatment of early-stage osteonecrosis of the femoral head (ONFH). GM interleukin-4 (IL4) over-expressing MSCs (IL4-MSCs), platelet-derived growth factor (PDGF)-BB over-expressing MSCs (PDGF-BB-MSCs), and IL4-PDGF-BB co-over-expressing MSCs (IL4-PDGF-BB-MSCs) and their respective pMSCs were used in this in vitro study and compared with respect to cell proliferation and osteogenic differentiation. IL4-MSCs, PDGF-BB-MSCs, IL4-PDGF-BB-MSCs, and each pMSC treatment significantly increased cell proliferation compared to the MSC group alone. The percentage of Alizarin red-stained area in the IL4-MSC and IL4-pMSC groups was significantly lower than in the MSC group. However, the percentage of Alizarin red-stained area in the PDGF-BB-MSC group was significantly higher than in the MSC and PDGF-BB-pMSC groups. The percentage of Alizarin red-stained area in the IL4-PDGF-BB-pMSC was significantly higher than in the IL4-PDGF-BB-MSC group. There were no significant differences in the percentage of Alizarin red-stained area between the MSC and IL4-PDGF-BB-pMSC groups. The use of PDGF-BB-MSCs or IL4-PDGF-BB-pMSCs increased cell proliferation. Furthermore, PDGF-BB-MSCs promoted osteogenic differentiation. The addition of GM MSCs may provide a useful supplementary cell-based therapy to CD for treatment of ONFH.Masanori TsubosakaMasahiro MaruyamaElijah Ejun HuangNing ZhangTakeshi UtsunomiyaQi GaoHuaishuang ShenXueping LiJunichi KushiokaHirohito HirataZhenyu YaoYunzhi Peter YangStuart B. GoodmanMDPI AGarticlemesenchymal stromal cellsgenetically modifiedpreconditioningosteonecrosiscore decompressionTechnologyTBiology (General)QH301-705.5ENBioengineering, Vol 8, Iss 165, p 165 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mesenchymal stromal cells genetically modified preconditioning osteonecrosis core decompression Technology T Biology (General) QH301-705.5
spellingShingle	mesenchymal stromal cells genetically modified preconditioning osteonecrosis core decompression Technology T Biology (General) QH301-705.5 Masanori Tsubosaka Masahiro Maruyama Elijah Ejun Huang Ning Zhang Takeshi Utsunomiya Qi Gao Huaishuang Shen Xueping Li Junichi Kushioka Hirohito Hirata Zhenyu Yao Yunzhi Peter Yang Stuart B. Goodman Effect on Osteogenic Differentiation of Genetically Modified IL4 or PDGF-BB Over-Expressing and IL4-PDGF-BB Co-Over-Expressing Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro
description	The use of genetically modified (GM) mesenchymal stromal cells (MSCs) and preconditioned MSCs (pMSCs) may provide further opportunities to improve the outcome of core decompression (CD) for the treatment of early-stage osteonecrosis of the femoral head (ONFH). GM interleukin-4 (IL4) over-expressing MSCs (IL4-MSCs), platelet-derived growth factor (PDGF)-BB over-expressing MSCs (PDGF-BB-MSCs), and IL4-PDGF-BB co-over-expressing MSCs (IL4-PDGF-BB-MSCs) and their respective pMSCs were used in this in vitro study and compared with respect to cell proliferation and osteogenic differentiation. IL4-MSCs, PDGF-BB-MSCs, IL4-PDGF-BB-MSCs, and each pMSC treatment significantly increased cell proliferation compared to the MSC group alone. The percentage of Alizarin red-stained area in the IL4-MSC and IL4-pMSC groups was significantly lower than in the MSC group. However, the percentage of Alizarin red-stained area in the PDGF-BB-MSC group was significantly higher than in the MSC and PDGF-BB-pMSC groups. The percentage of Alizarin red-stained area in the IL4-PDGF-BB-pMSC was significantly higher than in the IL4-PDGF-BB-MSC group. There were no significant differences in the percentage of Alizarin red-stained area between the MSC and IL4-PDGF-BB-pMSC groups. The use of PDGF-BB-MSCs or IL4-PDGF-BB-pMSCs increased cell proliferation. Furthermore, PDGF-BB-MSCs promoted osteogenic differentiation. The addition of GM MSCs may provide a useful supplementary cell-based therapy to CD for treatment of ONFH.
format	article
author	Masanori Tsubosaka Masahiro Maruyama Elijah Ejun Huang Ning Zhang Takeshi Utsunomiya Qi Gao Huaishuang Shen Xueping Li Junichi Kushioka Hirohito Hirata Zhenyu Yao Yunzhi Peter Yang Stuart B. Goodman
author_facet	Masanori Tsubosaka Masahiro Maruyama Elijah Ejun Huang Ning Zhang Takeshi Utsunomiya Qi Gao Huaishuang Shen Xueping Li Junichi Kushioka Hirohito Hirata Zhenyu Yao Yunzhi Peter Yang Stuart B. Goodman
author_sort	Masanori Tsubosaka
title	Effect on Osteogenic Differentiation of Genetically Modified IL4 or PDGF-BB Over-Expressing and IL4-PDGF-BB Co-Over-Expressing Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro
title_short	Effect on Osteogenic Differentiation of Genetically Modified IL4 or PDGF-BB Over-Expressing and IL4-PDGF-BB Co-Over-Expressing Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro
title_full	Effect on Osteogenic Differentiation of Genetically Modified IL4 or PDGF-BB Over-Expressing and IL4-PDGF-BB Co-Over-Expressing Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro
title_fullStr	Effect on Osteogenic Differentiation of Genetically Modified IL4 or PDGF-BB Over-Expressing and IL4-PDGF-BB Co-Over-Expressing Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro
title_full_unstemmed	Effect on Osteogenic Differentiation of Genetically Modified IL4 or PDGF-BB Over-Expressing and IL4-PDGF-BB Co-Over-Expressing Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro
title_sort	effect on osteogenic differentiation of genetically modified il4 or pdgf-bb over-expressing and il4-pdgf-bb co-over-expressing bone marrow-derived mesenchymal stromal cells in vitro
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8acc8113415f4067b5fcfc36b60d7aa4
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Effect on Osteogenic Differentiation of Genetically Modified IL4 or PDGF-BB Over-Expressing and IL4-PDGF-BB Co-Over-Expressing Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro

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