The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics

Abstract This study proposed a new experimental approach for the vascular and phenotype evaluation of the non-anesthetized zebrafish with representative imaging orientations for heart, pectoral fin beating, and vasculature views by means of the designed microfluidic device through inducing the optom...

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Autores principales:	Satishkumar Subendran, Yi-Chieh Wang, Yueh-Hsun Lu, Chia-Yuan Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8acecfae3a674fb4b832ae230530496f
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spelling	oai:doaj.org-article:8acecfae3a674fb4b832ae230530496f2021-12-02T18:34:20ZThe evaluation of zebrafish cardiovascular and behavioral functions through microfluidics10.1038/s41598-021-93078-y2045-2322https://doaj.org/article/8acecfae3a674fb4b832ae230530496f2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93078-yhttps://doaj.org/toc/2045-2322Abstract This study proposed a new experimental approach for the vascular and phenotype evaluation of the non-anesthetized zebrafish with representative imaging orientations for heart, pectoral fin beating, and vasculature views by means of the designed microfluidic device through inducing the optomotor response and hydrodynamic pressure control. In order to provide the visual cues for better positioning of zebrafish, computer-animated moving grids were generated by an in-house control interface which was powered by the larval optomotor response, in conjunction with the pressure suction control. The presented platform provided a comprehensive evaluation of internal circulation and the linked external behaviors of zebrafish in response to the cardiovascular parameter changes. The insights from these imaging sections was extended to identify the linkage between the cardiac parameters and behavioral endpoints. In addition, selected chemicals such as ethanol and caffeine were employed for the treatment of zebrafish. The obtained findings can be applicable for future investigation in behavioral drug screening serving as the forefront in psychopharmacological and cognition research.Satishkumar SubendranYi-Chieh WangYueh-Hsun LuChia-Yuan ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Satishkumar Subendran Yi-Chieh Wang Yueh-Hsun Lu Chia-Yuan Chen The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics
description	Abstract This study proposed a new experimental approach for the vascular and phenotype evaluation of the non-anesthetized zebrafish with representative imaging orientations for heart, pectoral fin beating, and vasculature views by means of the designed microfluidic device through inducing the optomotor response and hydrodynamic pressure control. In order to provide the visual cues for better positioning of zebrafish, computer-animated moving grids were generated by an in-house control interface which was powered by the larval optomotor response, in conjunction with the pressure suction control. The presented platform provided a comprehensive evaluation of internal circulation and the linked external behaviors of zebrafish in response to the cardiovascular parameter changes. The insights from these imaging sections was extended to identify the linkage between the cardiac parameters and behavioral endpoints. In addition, selected chemicals such as ethanol and caffeine were employed for the treatment of zebrafish. The obtained findings can be applicable for future investigation in behavioral drug screening serving as the forefront in psychopharmacological and cognition research.
format	article
author	Satishkumar Subendran Yi-Chieh Wang Yueh-Hsun Lu Chia-Yuan Chen
author_facet	Satishkumar Subendran Yi-Chieh Wang Yueh-Hsun Lu Chia-Yuan Chen
author_sort	Satishkumar Subendran
title	The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics
title_short	The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics
title_full	The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics
title_fullStr	The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics
title_full_unstemmed	The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics
title_sort	evaluation of zebrafish cardiovascular and behavioral functions through microfluidics
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8acecfae3a674fb4b832ae230530496f
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The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics

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