A novel family of mammalian transmembrane proteins involved in cholesterol transport

Abstract Cholesterol is an essential compound in mammalian cells because it is involved in a wide range of functions, including as a key component of membranes, precursor of important molecules such as hormones, bile acids and vitamin D. The cholesterol transport across the circulatory system is a w...

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Autores principales:	Kevin M. Méndez-Acevedo, Victor Julián Valdes, Alexander Asanov, Luis Vaca
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8acff224168641cdbf1d0d72744cdb9a
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spelling	oai:doaj.org-article:8acff224168641cdbf1d0d72744cdb9a2021-12-02T11:53:08ZA novel family of mammalian transmembrane proteins involved in cholesterol transport10.1038/s41598-017-07077-z2045-2322https://doaj.org/article/8acff224168641cdbf1d0d72744cdb9a2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07077-zhttps://doaj.org/toc/2045-2322Abstract Cholesterol is an essential compound in mammalian cells because it is involved in a wide range of functions, including as a key component of membranes, precursor of important molecules such as hormones, bile acids and vitamin D. The cholesterol transport across the circulatory system is a well-known process in contrast to the intracellular cholesterol transport, which is poorly understood. Recently in our laboratory, we identified a novel protein in C. elegans involved in dietary cholesterol uptake, which we have named ChUP-1. Insillicoanalysis identified two putative orthologue candidate proteins in mammals. The proteins SIDT1 and SIDT2 share identity and conserved cholesterol binding (CRAC) domains with C. elegans ChUP-1. Both mammalian proteins are annotated as RNA transporters in databases. In the present study, we show evidence indicating that SIDT1 and SIDT2 not only do not transport RNA, but they are involved in cholesterol transport. Furthermore, we show that single point mutations directed to disrupt the CRAC domains of both proteins prevent FRET between SIDT1 and SIDT2 and the cholesterol analogue dehydroergosterol (DHE) and alter cholesterol transport.Kevin M. Méndez-AcevedoVictor Julián ValdesAlexander AsanovLuis VacaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kevin M. Méndez-Acevedo Victor Julián Valdes Alexander Asanov Luis Vaca A novel family of mammalian transmembrane proteins involved in cholesterol transport
description	Abstract Cholesterol is an essential compound in mammalian cells because it is involved in a wide range of functions, including as a key component of membranes, precursor of important molecules such as hormones, bile acids and vitamin D. The cholesterol transport across the circulatory system is a well-known process in contrast to the intracellular cholesterol transport, which is poorly understood. Recently in our laboratory, we identified a novel protein in C. elegans involved in dietary cholesterol uptake, which we have named ChUP-1. Insillicoanalysis identified two putative orthologue candidate proteins in mammals. The proteins SIDT1 and SIDT2 share identity and conserved cholesterol binding (CRAC) domains with C. elegans ChUP-1. Both mammalian proteins are annotated as RNA transporters in databases. In the present study, we show evidence indicating that SIDT1 and SIDT2 not only do not transport RNA, but they are involved in cholesterol transport. Furthermore, we show that single point mutations directed to disrupt the CRAC domains of both proteins prevent FRET between SIDT1 and SIDT2 and the cholesterol analogue dehydroergosterol (DHE) and alter cholesterol transport.
format	article
author	Kevin M. Méndez-Acevedo Victor Julián Valdes Alexander Asanov Luis Vaca
author_facet	Kevin M. Méndez-Acevedo Victor Julián Valdes Alexander Asanov Luis Vaca
author_sort	Kevin M. Méndez-Acevedo
title	A novel family of mammalian transmembrane proteins involved in cholesterol transport
title_short	A novel family of mammalian transmembrane proteins involved in cholesterol transport
title_full	A novel family of mammalian transmembrane proteins involved in cholesterol transport
title_fullStr	A novel family of mammalian transmembrane proteins involved in cholesterol transport
title_full_unstemmed	A novel family of mammalian transmembrane proteins involved in cholesterol transport
title_sort	novel family of mammalian transmembrane proteins involved in cholesterol transport
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8acff224168641cdbf1d0d72744cdb9a
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A novel family of mammalian transmembrane proteins involved in cholesterol transport

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