Identification and comparative expression analysis of odorant-binding proteins in the reproductive system and antennae of Athetis dissimilis

Abstract Odorant-binding proteins (OBPs) are prevalent in the antennal transcriptomes of different orders of insects. Studies on OBPs have focused on their role in the insect chemosensory system, but knowledge of their functions in the insect testis is limited. We sequenced the transcriptomes of the...

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Autores principales:	Yue-Qin Song, Zhi-Yu Song, Jun-Feng Dong, Qi-Hui Lv, Qing-Xiao Chen, Hui-Zhong Sun
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8ad04feff36e429eb50712d3a9196bd0
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spelling	oai:doaj.org-article:8ad04feff36e429eb50712d3a9196bd02021-12-02T16:14:55ZIdentification and comparative expression analysis of odorant-binding proteins in the reproductive system and antennae of Athetis dissimilis10.1038/s41598-021-93423-12045-2322https://doaj.org/article/8ad04feff36e429eb50712d3a9196bd02021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93423-1https://doaj.org/toc/2045-2322Abstract Odorant-binding proteins (OBPs) are prevalent in the antennal transcriptomes of different orders of insects. Studies on OBPs have focused on their role in the insect chemosensory system, but knowledge of their functions in the insect testis is limited. We sequenced the transcriptomes of the Athetis dissimilis reproductive organs and analyzed the expression of AdisOBP genes in different tissues. We identified 23 OBPs in the testis and ovaries and 31 OBPs in antennal transcriptomes. The results of real-time quantitative PCR revealed that 23 of the 54 OBP genes were highly expressed in both female and male antennae, including three that exhibited male-biased expression and 15 that exhibited female-biased expression. A total of 24 OBPs were highly expressed in the testis of A. dissimilis, while expression of OBPs in the ovaries was very low. These findings highlight the functional diversity of OBPs in insects and can facilitate further studies on the OBPs in A. dissimilis and lepidopteran species.Yue-Qin SongZhi-Yu SongJun-Feng DongQi-Hui LvQing-Xiao ChenHui-Zhong SunNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yue-Qin Song Zhi-Yu Song Jun-Feng Dong Qi-Hui Lv Qing-Xiao Chen Hui-Zhong Sun Identification and comparative expression analysis of odorant-binding proteins in the reproductive system and antennae of Athetis dissimilis
description	Abstract Odorant-binding proteins (OBPs) are prevalent in the antennal transcriptomes of different orders of insects. Studies on OBPs have focused on their role in the insect chemosensory system, but knowledge of their functions in the insect testis is limited. We sequenced the transcriptomes of the Athetis dissimilis reproductive organs and analyzed the expression of AdisOBP genes in different tissues. We identified 23 OBPs in the testis and ovaries and 31 OBPs in antennal transcriptomes. The results of real-time quantitative PCR revealed that 23 of the 54 OBP genes were highly expressed in both female and male antennae, including three that exhibited male-biased expression and 15 that exhibited female-biased expression. A total of 24 OBPs were highly expressed in the testis of A. dissimilis, while expression of OBPs in the ovaries was very low. These findings highlight the functional diversity of OBPs in insects and can facilitate further studies on the OBPs in A. dissimilis and lepidopteran species.
format	article
author	Yue-Qin Song Zhi-Yu Song Jun-Feng Dong Qi-Hui Lv Qing-Xiao Chen Hui-Zhong Sun
author_facet	Yue-Qin Song Zhi-Yu Song Jun-Feng Dong Qi-Hui Lv Qing-Xiao Chen Hui-Zhong Sun
author_sort	Yue-Qin Song
title	Identification and comparative expression analysis of odorant-binding proteins in the reproductive system and antennae of Athetis dissimilis
title_short	Identification and comparative expression analysis of odorant-binding proteins in the reproductive system and antennae of Athetis dissimilis
title_full	Identification and comparative expression analysis of odorant-binding proteins in the reproductive system and antennae of Athetis dissimilis
title_fullStr	Identification and comparative expression analysis of odorant-binding proteins in the reproductive system and antennae of Athetis dissimilis
title_full_unstemmed	Identification and comparative expression analysis of odorant-binding proteins in the reproductive system and antennae of Athetis dissimilis
title_sort	identification and comparative expression analysis of odorant-binding proteins in the reproductive system and antennae of athetis dissimilis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8ad04feff36e429eb50712d3a9196bd0
work_keys_str_mv	AT yueqinsong identificationandcomparativeexpressionanalysisofodorantbindingproteinsinthereproductivesystemandantennaeofathetisdissimilis AT zhiyusong identificationandcomparativeexpressionanalysisofodorantbindingproteinsinthereproductivesystemandantennaeofathetisdissimilis AT junfengdong identificationandcomparativeexpressionanalysisofodorantbindingproteinsinthereproductivesystemandantennaeofathetisdissimilis AT qihuilv identificationandcomparativeexpressionanalysisofodorantbindingproteinsinthereproductivesystemandantennaeofathetisdissimilis AT qingxiaochen identificationandcomparativeexpressionanalysisofodorantbindingproteinsinthereproductivesystemandantennaeofathetisdissimilis AT huizhongsun identificationandcomparativeexpressionanalysisofodorantbindingproteinsinthereproductivesystemandantennaeofathetisdissimilis
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Identification and comparative expression analysis of odorant-binding proteins in the reproductive system and antennae of Athetis dissimilis

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