Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9

Abstract Parthenocarpy in horticultural crop plants is an important trait with agricultural value for various industrial purposes as well as direct eating quality. Here, we demonstrate a breeding strategy to generate parthenocarpic tomato plants using the CRISPR/Cas9 system. We optimized the CRISPR/...

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Autores principales:	Risa Ueta, Chihiro Abe, Takahito Watanabe, Shigeo S. Sugano, Ryosuke Ishihara, Hiroshi Ezura, Yuriko Osakabe, Keishi Osakabe
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8ad056f11a024f9098a24705ca822fbe
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spelling	oai:doaj.org-article:8ad056f11a024f9098a24705ca822fbe2021-12-02T15:05:57ZRapid breeding of parthenocarpic tomato plants using CRISPR/Cas910.1038/s41598-017-00501-42045-2322https://doaj.org/article/8ad056f11a024f9098a24705ca822fbe2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00501-4https://doaj.org/toc/2045-2322Abstract Parthenocarpy in horticultural crop plants is an important trait with agricultural value for various industrial purposes as well as direct eating quality. Here, we demonstrate a breeding strategy to generate parthenocarpic tomato plants using the CRISPR/Cas9 system. We optimized the CRISPR/Cas9 system to introduce somatic mutations effectively into SlIAA9—a key gene controlling parthenocarpy—with mutation rates of up to 100% in the T0 generation. Furthermore, analysis of off-target mutations using deep sequencing indicated that our customized gRNAs induced no additional mutations in the host genome. Regenerated mutants exhibited morphological changes in leaf shape and seedless fruit—a characteristic of parthenocarpic tomato. And the segregated next generation (T1) also showed a severe phenotype associated with the homozygous mutated genome. The system developed here could be applied to produce parthenocarpic tomato in a wide variety of cultivars, as well as other major horticultural crops, using this precise and rapid breeding technique.Risa UetaChihiro AbeTakahito WatanabeShigeo S. SuganoRyosuke IshiharaHiroshi EzuraYuriko OsakabeKeishi OsakabeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Risa Ueta Chihiro Abe Takahito Watanabe Shigeo S. Sugano Ryosuke Ishihara Hiroshi Ezura Yuriko Osakabe Keishi Osakabe Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9
description	Abstract Parthenocarpy in horticultural crop plants is an important trait with agricultural value for various industrial purposes as well as direct eating quality. Here, we demonstrate a breeding strategy to generate parthenocarpic tomato plants using the CRISPR/Cas9 system. We optimized the CRISPR/Cas9 system to introduce somatic mutations effectively into SlIAA9—a key gene controlling parthenocarpy—with mutation rates of up to 100% in the T0 generation. Furthermore, analysis of off-target mutations using deep sequencing indicated that our customized gRNAs induced no additional mutations in the host genome. Regenerated mutants exhibited morphological changes in leaf shape and seedless fruit—a characteristic of parthenocarpic tomato. And the segregated next generation (T1) also showed a severe phenotype associated with the homozygous mutated genome. The system developed here could be applied to produce parthenocarpic tomato in a wide variety of cultivars, as well as other major horticultural crops, using this precise and rapid breeding technique.
format	article
author	Risa Ueta Chihiro Abe Takahito Watanabe Shigeo S. Sugano Ryosuke Ishihara Hiroshi Ezura Yuriko Osakabe Keishi Osakabe
author_facet	Risa Ueta Chihiro Abe Takahito Watanabe Shigeo S. Sugano Ryosuke Ishihara Hiroshi Ezura Yuriko Osakabe Keishi Osakabe
author_sort	Risa Ueta
title	Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9
title_short	Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9
title_full	Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9
title_fullStr	Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9
title_full_unstemmed	Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9
title_sort	rapid breeding of parthenocarpic tomato plants using crispr/cas9
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8ad056f11a024f9098a24705ca822fbe
work_keys_str_mv	AT risaueta rapidbreedingofparthenocarpictomatoplantsusingcrisprcas9 AT chihiroabe rapidbreedingofparthenocarpictomatoplantsusingcrisprcas9 AT takahitowatanabe rapidbreedingofparthenocarpictomatoplantsusingcrisprcas9 AT shigeossugano rapidbreedingofparthenocarpictomatoplantsusingcrisprcas9 AT ryosukeishihara rapidbreedingofparthenocarpictomatoplantsusingcrisprcas9 AT hiroshiezura rapidbreedingofparthenocarpictomatoplantsusingcrisprcas9 AT yurikoosakabe rapidbreedingofparthenocarpictomatoplantsusingcrisprcas9 AT keishiosakabe rapidbreedingofparthenocarpictomatoplantsusingcrisprcas9
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Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9

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