Mapping genetic variants underlying differences in the central nitrogen metabolism in fermenter yeasts.

Different populations within a species represent a rich reservoir of allelic variants, corresponding to an evolutionary signature of withstood environmental constraints. Saccharomyces cerevisiae strains are widely utilised in the fermentation of different kinds of alcoholic beverages, such as, wine...

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Autores principales:	Matías Jara, Francisco A Cubillos, Verónica García, Francisco Salinas, Omayra Aguilera, Gianni Liti, Claudio Martínez
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8ad1f030602c4521944c061f5183c9af
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spelling	oai:doaj.org-article:8ad1f030602c4521944c061f5183c9af2021-11-18T08:36:45ZMapping genetic variants underlying differences in the central nitrogen metabolism in fermenter yeasts.1932-620310.1371/journal.pone.0086533https://doaj.org/article/8ad1f030602c4521944c061f5183c9af2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24466135/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Different populations within a species represent a rich reservoir of allelic variants, corresponding to an evolutionary signature of withstood environmental constraints. Saccharomyces cerevisiae strains are widely utilised in the fermentation of different kinds of alcoholic beverages, such as, wine and sake, each of them derived from must with distinct nutrient composition. Importantly, adequate nitrogen levels in the medium are essential for the fermentation process, however, a comprehensive understanding of the genetic variants determining variation in nitrogen consumption is lacking. Here, we assessed the genetic factors underlying variation in nitrogen consumption in a segregating population derived from a cross between two main fermenter yeasts, a Wine/European and a Sake isolate. By linkage analysis we identified 18 main effect QTLs for ammonium and amino acids sources. Interestingly, majority of QTLs were involved in more than a single trait, grouped based on amino acid structure and indicating high levels of pleiotropy across nitrogen sources, in agreement with the observed patterns of phenotypic co-variation. Accordingly, we performed reciprocal hemizygosity analysis validating an effect for three genes, GLT1, ASI1 and AGP1. Furthermore, we detected a widespread pleiotropic effect on these genes, with AGP1 affecting seven amino acids and nine in the case of GLT1 and ASI1. Based on sequence and comparative analysis, candidate causative mutations within these genes were also predicted. Altogether, the identification of these variants demonstrate how Sake and Wine/European genetic backgrounds differentially consume nitrogen sources, in part explaining independently evolved preferences for nitrogen assimilation and representing a niche of genetic diversity for the implementation of practical approaches towards more efficient strains for nitrogen metabolism.Matías JaraFrancisco A CubillosVerónica GarcíaFrancisco SalinasOmayra AguileraGianni LitiClaudio MartínezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e86533 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Matías Jara Francisco A Cubillos Verónica García Francisco Salinas Omayra Aguilera Gianni Liti Claudio Martínez Mapping genetic variants underlying differences in the central nitrogen metabolism in fermenter yeasts.
description	Different populations within a species represent a rich reservoir of allelic variants, corresponding to an evolutionary signature of withstood environmental constraints. Saccharomyces cerevisiae strains are widely utilised in the fermentation of different kinds of alcoholic beverages, such as, wine and sake, each of them derived from must with distinct nutrient composition. Importantly, adequate nitrogen levels in the medium are essential for the fermentation process, however, a comprehensive understanding of the genetic variants determining variation in nitrogen consumption is lacking. Here, we assessed the genetic factors underlying variation in nitrogen consumption in a segregating population derived from a cross between two main fermenter yeasts, a Wine/European and a Sake isolate. By linkage analysis we identified 18 main effect QTLs for ammonium and amino acids sources. Interestingly, majority of QTLs were involved in more than a single trait, grouped based on amino acid structure and indicating high levels of pleiotropy across nitrogen sources, in agreement with the observed patterns of phenotypic co-variation. Accordingly, we performed reciprocal hemizygosity analysis validating an effect for three genes, GLT1, ASI1 and AGP1. Furthermore, we detected a widespread pleiotropic effect on these genes, with AGP1 affecting seven amino acids and nine in the case of GLT1 and ASI1. Based on sequence and comparative analysis, candidate causative mutations within these genes were also predicted. Altogether, the identification of these variants demonstrate how Sake and Wine/European genetic backgrounds differentially consume nitrogen sources, in part explaining independently evolved preferences for nitrogen assimilation and representing a niche of genetic diversity for the implementation of practical approaches towards more efficient strains for nitrogen metabolism.
format	article
author	Matías Jara Francisco A Cubillos Verónica García Francisco Salinas Omayra Aguilera Gianni Liti Claudio Martínez
author_facet	Matías Jara Francisco A Cubillos Verónica García Francisco Salinas Omayra Aguilera Gianni Liti Claudio Martínez
author_sort	Matías Jara
title	Mapping genetic variants underlying differences in the central nitrogen metabolism in fermenter yeasts.
title_short	Mapping genetic variants underlying differences in the central nitrogen metabolism in fermenter yeasts.
title_full	Mapping genetic variants underlying differences in the central nitrogen metabolism in fermenter yeasts.
title_fullStr	Mapping genetic variants underlying differences in the central nitrogen metabolism in fermenter yeasts.
title_full_unstemmed	Mapping genetic variants underlying differences in the central nitrogen metabolism in fermenter yeasts.
title_sort	mapping genetic variants underlying differences in the central nitrogen metabolism in fermenter yeasts.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/8ad1f030602c4521944c061f5183c9af
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Mapping genetic variants underlying differences in the central nitrogen metabolism in fermenter yeasts.

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