FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway

The family with sequence similarity 13 member A (FAM13A) gene has been discovered in recent years and is related to metabolism. In this study, the function of FAM13A in precursor adipocyte proliferation in Qinchuan cattle was investigated using fluorescence quantitative polymerase chain reaction (PC...

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Autores principales:	Chengcheng Liang, Guohua Wang, Sayed Haidar Abbas Raza, Xiaoyu Wang, Bingzhi Li, Wenzhen Zhang, Linsen Zan
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	fam13a hypoxia proliferation hif-1 pathway Diseases of the endocrine glands. Clinical endocrinology RC648-665 Cytology QH573-671 Physiology QP1-981
Acceso en línea:	https://doaj.org/article/8ad2ecac595844b58e8c9703e6ec8a32
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spelling	oai:doaj.org-article:8ad2ecac595844b58e8c9703e6ec8a322021-11-04T15:00:45ZFAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway2162-39452162-397X10.1080/21623945.2021.1986327https://doaj.org/article/8ad2ecac595844b58e8c9703e6ec8a322021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21623945.2021.1986327https://doaj.org/toc/2162-3945https://doaj.org/toc/2162-397XThe family with sequence similarity 13 member A (FAM13A) gene has been discovered in recent years and is related to metabolism. In this study, the function of FAM13A in precursor adipocyte proliferation in Qinchuan cattle was investigated using fluorescence quantitative polymerase chain reaction (PCR), western blotting, 5-ethynyl-2′-deoxyuridine staining, and other tests. FAM13A promoted precursor adipocyte proliferation. To determine the pathway FAM13A was involved in, transcriptome sequencing, fluorescence quantitative PCR, western blotting, and other tests were used, which identified the hypoxia inducible factor-1 (HIF-1) signalling pathway. Finally, cobalt chloride, a chemical mimic of hypoxia, was used to treat precursor adipocytes. mRNA and protein levels of FAM13A were significantly increased after hypoxia. Thus, FAM13A promoted bovine precursor adipocyte proliferation by inhibiting the HIF-1 signalling pathway, whereas chemically induced hypoxia negatively regulated FAM13A expression, regulating cell proliferation.Chengcheng LiangGuohua WangSayed Haidar Abbas RazaXiaoyu WangBingzhi LiWenzhen ZhangLinsen ZanTaylor & Francis Grouparticlefam13ahypoxiaproliferationhif-1 pathwayDiseases of the endocrine glands. Clinical endocrinologyRC648-665CytologyQH573-671PhysiologyQP1-981ENAdipocyte, Vol 10, Iss 1, Pp 546-557 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	fam13a hypoxia proliferation hif-1 pathway Diseases of the endocrine glands. Clinical endocrinology RC648-665 Cytology QH573-671 Physiology QP1-981
spellingShingle	fam13a hypoxia proliferation hif-1 pathway Diseases of the endocrine glands. Clinical endocrinology RC648-665 Cytology QH573-671 Physiology QP1-981 Chengcheng Liang Guohua Wang Sayed Haidar Abbas Raza Xiaoyu Wang Bingzhi Li Wenzhen Zhang Linsen Zan FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway
description	The family with sequence similarity 13 member A (FAM13A) gene has been discovered in recent years and is related to metabolism. In this study, the function of FAM13A in precursor adipocyte proliferation in Qinchuan cattle was investigated using fluorescence quantitative polymerase chain reaction (PCR), western blotting, 5-ethynyl-2′-deoxyuridine staining, and other tests. FAM13A promoted precursor adipocyte proliferation. To determine the pathway FAM13A was involved in, transcriptome sequencing, fluorescence quantitative PCR, western blotting, and other tests were used, which identified the hypoxia inducible factor-1 (HIF-1) signalling pathway. Finally, cobalt chloride, a chemical mimic of hypoxia, was used to treat precursor adipocytes. mRNA and protein levels of FAM13A were significantly increased after hypoxia. Thus, FAM13A promoted bovine precursor adipocyte proliferation by inhibiting the HIF-1 signalling pathway, whereas chemically induced hypoxia negatively regulated FAM13A expression, regulating cell proliferation.
format	article
author	Chengcheng Liang Guohua Wang Sayed Haidar Abbas Raza Xiaoyu Wang Bingzhi Li Wenzhen Zhang Linsen Zan
author_facet	Chengcheng Liang Guohua Wang Sayed Haidar Abbas Raza Xiaoyu Wang Bingzhi Li Wenzhen Zhang Linsen Zan
author_sort	Chengcheng Liang
title	FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway
title_short	FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway
title_full	FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway
title_fullStr	FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway
title_full_unstemmed	FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway
title_sort	fam13a promotes proliferation of bovine preadipocytes by targeting hypoxia-inducible factor-1 signaling pathway
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/8ad2ecac595844b58e8c9703e6ec8a32
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FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway

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