Selection and Characterization of Vimentin-Binding Aptamer Motifs for Ovarian Cancer

The application of aptamers in biomedicine is emerging as an essential technology in the field of cancer research. As small single-stranded DNA or RNA ligands with high specificity and low immunogenicity for their targets, aptamers provide many advantages in cancer therapeutics over protein-based mo...

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Autores principales:	Andrea M. Costello, Miguel-Angel Elizondo-Riojas, Xin Li, David E. Volk, Anil K. Pillai, Hongyu Wang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	aptamer binding motifs ovarian cancer Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/8ad852c2e154486eb8bd2225bd6512d1
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spelling	oai:doaj.org-article:8ad852c2e154486eb8bd2225bd6512d12021-11-11T18:30:52ZSelection and Characterization of Vimentin-Binding Aptamer Motifs for Ovarian Cancer10.3390/molecules262165251420-3049https://doaj.org/article/8ad852c2e154486eb8bd2225bd6512d12021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6525https://doaj.org/toc/1420-3049The application of aptamers in biomedicine is emerging as an essential technology in the field of cancer research. As small single-stranded DNA or RNA ligands with high specificity and low immunogenicity for their targets, aptamers provide many advantages in cancer therapeutics over protein-based molecules, such as antibodies. Vimentin is an intermediate filament protein that is overexpressed in endothelial cells of cancerous tissue. High expression levels of vimentin have been associated with increased capacity for migration and invasion of the tumor cells. We have selected and identified thioated aptamers with high specificity for vimentin using human ovarian cancer tissues. Tentative binding motifs were chosen for two vimentin aptamers based on predicted secondary structures. Each of these shorter, tentative binding motifs was synthesized, purified, and characterized via cell binding assays. Two vimentin binding motifs with high fidelity binding were selected and further characterized via cell and tissue binding assays, as well as flow cytometric analysis. The equilibrium binding constants of these small thioated aptamer constructs were also determined. Future applications for the vimentin binding aptamer motifs include conjugation of the aptamers to synthetic dyes for use in targeted imaging and therapy, and ultimately more detailed and precise monitoring of treatment response and tumor progression in ovarian pathology.Andrea M. CostelloMiguel-Angel Elizondo-RiojasXin LiDavid E. VolkAnil K. PillaiHongyu WangMDPI AGarticleaptamerbinding motifsovarian cancerOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6525, p 6525 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	aptamer binding motifs ovarian cancer Organic chemistry QD241-441
spellingShingle	aptamer binding motifs ovarian cancer Organic chemistry QD241-441 Andrea M. Costello Miguel-Angel Elizondo-Riojas Xin Li David E. Volk Anil K. Pillai Hongyu Wang Selection and Characterization of Vimentin-Binding Aptamer Motifs for Ovarian Cancer
description	The application of aptamers in biomedicine is emerging as an essential technology in the field of cancer research. As small single-stranded DNA or RNA ligands with high specificity and low immunogenicity for their targets, aptamers provide many advantages in cancer therapeutics over protein-based molecules, such as antibodies. Vimentin is an intermediate filament protein that is overexpressed in endothelial cells of cancerous tissue. High expression levels of vimentin have been associated with increased capacity for migration and invasion of the tumor cells. We have selected and identified thioated aptamers with high specificity for vimentin using human ovarian cancer tissues. Tentative binding motifs were chosen for two vimentin aptamers based on predicted secondary structures. Each of these shorter, tentative binding motifs was synthesized, purified, and characterized via cell binding assays. Two vimentin binding motifs with high fidelity binding were selected and further characterized via cell and tissue binding assays, as well as flow cytometric analysis. The equilibrium binding constants of these small thioated aptamer constructs were also determined. Future applications for the vimentin binding aptamer motifs include conjugation of the aptamers to synthetic dyes for use in targeted imaging and therapy, and ultimately more detailed and precise monitoring of treatment response and tumor progression in ovarian pathology.
format	article
author	Andrea M. Costello Miguel-Angel Elizondo-Riojas Xin Li David E. Volk Anil K. Pillai Hongyu Wang
author_facet	Andrea M. Costello Miguel-Angel Elizondo-Riojas Xin Li David E. Volk Anil K. Pillai Hongyu Wang
author_sort	Andrea M. Costello
title	Selection and Characterization of Vimentin-Binding Aptamer Motifs for Ovarian Cancer
title_short	Selection and Characterization of Vimentin-Binding Aptamer Motifs for Ovarian Cancer
title_full	Selection and Characterization of Vimentin-Binding Aptamer Motifs for Ovarian Cancer
title_fullStr	Selection and Characterization of Vimentin-Binding Aptamer Motifs for Ovarian Cancer
title_full_unstemmed	Selection and Characterization of Vimentin-Binding Aptamer Motifs for Ovarian Cancer
title_sort	selection and characterization of vimentin-binding aptamer motifs for ovarian cancer
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8ad852c2e154486eb8bd2225bd6512d1
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Selection and Characterization of Vimentin-Binding Aptamer Motifs for Ovarian Cancer

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