Klebsiella phage vB_KleM-RaK2 - a giant singleton virus of the family Myoviridae.

At 346 kbp in size, the genome of a jumbo bacteriophage vB_KleM-RaK2 (RaK2) is the largest Klebsiella infecting myovirus genome sequenced to date. In total, 272 out of 534 RaK2 ORFs lack detectable database homologues. Based on the similarity to biologically defined proteins and/or MS/MS analysis, 1...

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Autores principales:	Eugenijus Simoliūnas, Laura Kaliniene, Lidija Truncaitė, Aurelija Zajančkauskaitė, Juozas Staniulis, Algirdas Kaupinis, Marija Ger, Mindaugas Valius, Rolandas Meškys
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8adb0b69871e487fa8c42197ac88cb6a
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spelling	oai:doaj.org-article:8adb0b69871e487fa8c42197ac88cb6a2021-11-18T07:49:54ZKlebsiella phage vB_KleM-RaK2 - a giant singleton virus of the family Myoviridae.1932-620310.1371/journal.pone.0060717https://doaj.org/article/8adb0b69871e487fa8c42197ac88cb6a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23593293/?tool=EBIhttps://doaj.org/toc/1932-6203At 346 kbp in size, the genome of a jumbo bacteriophage vB_KleM-RaK2 (RaK2) is the largest Klebsiella infecting myovirus genome sequenced to date. In total, 272 out of 534 RaK2 ORFs lack detectable database homologues. Based on the similarity to biologically defined proteins and/or MS/MS analysis, 117 of RaK2 ORFs were given a functional annotation, including 28 RaK2 ORFs coding for structural proteins that have no reliable homologues to annotated structural proteins in other organisms. The electron micrographs revealed elaborate spike-like structures on the tail fibers of Rak2, suggesting that this phage is an atypical myovirus. While head and tail proteins of RaK2 are mostly myoviridae-related, the bioinformatics analysis indicate that tail fibers/spikes of this phage are formed from podovirus-like peptides predominantly. Overall, these results provide evidence that bacteriophage RaK2 differs profoundly from previously studied viruses of the Myoviridae family.Eugenijus SimoliūnasLaura KalinieneLidija TruncaitėAurelija ZajančkauskaitėJuozas StaniulisAlgirdas KaupinisMarija GerMindaugas ValiusRolandas MeškysPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e60717 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eugenijus Simoliūnas Laura Kaliniene Lidija Truncaitė Aurelija Zajančkauskaitė Juozas Staniulis Algirdas Kaupinis Marija Ger Mindaugas Valius Rolandas Meškys Klebsiella phage vB_KleM-RaK2 - a giant singleton virus of the family Myoviridae.
description	At 346 kbp in size, the genome of a jumbo bacteriophage vB_KleM-RaK2 (RaK2) is the largest Klebsiella infecting myovirus genome sequenced to date. In total, 272 out of 534 RaK2 ORFs lack detectable database homologues. Based on the similarity to biologically defined proteins and/or MS/MS analysis, 117 of RaK2 ORFs were given a functional annotation, including 28 RaK2 ORFs coding for structural proteins that have no reliable homologues to annotated structural proteins in other organisms. The electron micrographs revealed elaborate spike-like structures on the tail fibers of Rak2, suggesting that this phage is an atypical myovirus. While head and tail proteins of RaK2 are mostly myoviridae-related, the bioinformatics analysis indicate that tail fibers/spikes of this phage are formed from podovirus-like peptides predominantly. Overall, these results provide evidence that bacteriophage RaK2 differs profoundly from previously studied viruses of the Myoviridae family.
format	article
author	Eugenijus Simoliūnas Laura Kaliniene Lidija Truncaitė Aurelija Zajančkauskaitė Juozas Staniulis Algirdas Kaupinis Marija Ger Mindaugas Valius Rolandas Meškys
author_facet	Eugenijus Simoliūnas Laura Kaliniene Lidija Truncaitė Aurelija Zajančkauskaitė Juozas Staniulis Algirdas Kaupinis Marija Ger Mindaugas Valius Rolandas Meškys
author_sort	Eugenijus Simoliūnas
title	Klebsiella phage vB_KleM-RaK2 - a giant singleton virus of the family Myoviridae.
title_short	Klebsiella phage vB_KleM-RaK2 - a giant singleton virus of the family Myoviridae.
title_full	Klebsiella phage vB_KleM-RaK2 - a giant singleton virus of the family Myoviridae.
title_fullStr	Klebsiella phage vB_KleM-RaK2 - a giant singleton virus of the family Myoviridae.
title_full_unstemmed	Klebsiella phage vB_KleM-RaK2 - a giant singleton virus of the family Myoviridae.
title_sort	klebsiella phage vb_klem-rak2 - a giant singleton virus of the family myoviridae.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/8adb0b69871e487fa8c42197ac88cb6a
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Klebsiella phage vB_KleM-RaK2 - a giant singleton virus of the family Myoviridae.

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