Complex Responses to Hydrogen Peroxide and Hypochlorous Acid by the Probiotic Bacterium <named-content content-type="genus-species">Lactobacillus reuteri</named-content>

ABSTRACT Inflammatory diseases of the gut are associated with increased intestinal oxygen concentrations and high levels of inflammatory oxidants, including hydrogen peroxide (H2O2) and hypochlorous acid (HOCl), which are antimicrobial compounds produced by the innate immune system. This contributes...

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Autores principales:	Poulami Basu Thakur, Abagail R. Long, Benjamin J. Nelson, Ranjit Kumar, Alexander F. Rosenberg, Michael J. Gray
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	oxidative stress probiotics transcriptomics Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/8ae048719e8341598290cf3855a23cc3
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spelling	oai:doaj.org-article:8ae048719e8341598290cf3855a23cc32021-12-02T18:39:15ZComplex Responses to Hydrogen Peroxide and Hypochlorous Acid by the Probiotic Bacterium <named-content content-type="genus-species">Lactobacillus reuteri</named-content>10.1128/mSystems.00453-192379-5077https://doaj.org/article/8ae048719e8341598290cf3855a23cc32019-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00453-19https://doaj.org/toc/2379-5077ABSTRACT Inflammatory diseases of the gut are associated with increased intestinal oxygen concentrations and high levels of inflammatory oxidants, including hydrogen peroxide (H2O2) and hypochlorous acid (HOCl), which are antimicrobial compounds produced by the innate immune system. This contributes to dysbiotic changes in the gut microbiome, including increased populations of proinflammatory enterobacteria (Escherichia coli and related species) and decreased levels of health-associated anaerobic Firmicutes and Bacteroidetes. The pathways for H2O2 and HOCl resistance in E. coli have been well studied, but little is known about how commensal and probiotic bacteria respond to inflammatory oxidants. In this work, we have characterized the transcriptomic response of the anti-inflammatory, gut-colonizing Gram-positive probiotic Lactobacillus reuteri to both H2O2 and HOCl. L. reuteri mounts distinct but overlapping responses to each of these stressors, and both gene expression and survival were strongly affected by the presence or absence of oxygen. Oxidative stress response in L. reuteri required several factors not found in enterobacteria, including the small heat shock protein Lo18, polyphosphate kinase 2, and RsiR, an L. reuteri-specific regulator of anti-inflammatory mechanisms. IMPORTANCE Reactive oxidants, including hydrogen peroxide and hypochlorous acid, are antimicrobial compounds produced by the immune system during inflammation. Little is known, however, about how many important types of bacteria present in the human microbiome respond to these oxidants, especially commensal and other health-associated species. We have now mapped the stress response to both H2O2 and HOCl in the intestinal lactic acid bacterium Lactobacillus reuteri.Poulami Basu ThakurAbagail R. LongBenjamin J. NelsonRanjit KumarAlexander F. RosenbergMichael J. GrayAmerican Society for Microbiologyarticleoxidative stressprobioticstranscriptomicsMicrobiologyQR1-502ENmSystems, Vol 4, Iss 5 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	oxidative stress probiotics transcriptomics Microbiology QR1-502
spellingShingle	oxidative stress probiotics transcriptomics Microbiology QR1-502 Poulami Basu Thakur Abagail R. Long Benjamin J. Nelson Ranjit Kumar Alexander F. Rosenberg Michael J. Gray Complex Responses to Hydrogen Peroxide and Hypochlorous Acid by the Probiotic Bacterium <named-content content-type="genus-species">Lactobacillus reuteri</named-content>
description	ABSTRACT Inflammatory diseases of the gut are associated with increased intestinal oxygen concentrations and high levels of inflammatory oxidants, including hydrogen peroxide (H2O2) and hypochlorous acid (HOCl), which are antimicrobial compounds produced by the innate immune system. This contributes to dysbiotic changes in the gut microbiome, including increased populations of proinflammatory enterobacteria (Escherichia coli and related species) and decreased levels of health-associated anaerobic Firmicutes and Bacteroidetes. The pathways for H2O2 and HOCl resistance in E. coli have been well studied, but little is known about how commensal and probiotic bacteria respond to inflammatory oxidants. In this work, we have characterized the transcriptomic response of the anti-inflammatory, gut-colonizing Gram-positive probiotic Lactobacillus reuteri to both H2O2 and HOCl. L. reuteri mounts distinct but overlapping responses to each of these stressors, and both gene expression and survival were strongly affected by the presence or absence of oxygen. Oxidative stress response in L. reuteri required several factors not found in enterobacteria, including the small heat shock protein Lo18, polyphosphate kinase 2, and RsiR, an L. reuteri-specific regulator of anti-inflammatory mechanisms. IMPORTANCE Reactive oxidants, including hydrogen peroxide and hypochlorous acid, are antimicrobial compounds produced by the immune system during inflammation. Little is known, however, about how many important types of bacteria present in the human microbiome respond to these oxidants, especially commensal and other health-associated species. We have now mapped the stress response to both H2O2 and HOCl in the intestinal lactic acid bacterium Lactobacillus reuteri.
format	article
author	Poulami Basu Thakur Abagail R. Long Benjamin J. Nelson Ranjit Kumar Alexander F. Rosenberg Michael J. Gray
author_facet	Poulami Basu Thakur Abagail R. Long Benjamin J. Nelson Ranjit Kumar Alexander F. Rosenberg Michael J. Gray
author_sort	Poulami Basu Thakur
title	Complex Responses to Hydrogen Peroxide and Hypochlorous Acid by the Probiotic Bacterium <named-content content-type="genus-species">Lactobacillus reuteri</named-content>
title_short	Complex Responses to Hydrogen Peroxide and Hypochlorous Acid by the Probiotic Bacterium <named-content content-type="genus-species">Lactobacillus reuteri</named-content>
title_full	Complex Responses to Hydrogen Peroxide and Hypochlorous Acid by the Probiotic Bacterium <named-content content-type="genus-species">Lactobacillus reuteri</named-content>
title_fullStr	Complex Responses to Hydrogen Peroxide and Hypochlorous Acid by the Probiotic Bacterium <named-content content-type="genus-species">Lactobacillus reuteri</named-content>
title_full_unstemmed	Complex Responses to Hydrogen Peroxide and Hypochlorous Acid by the Probiotic Bacterium <named-content content-type="genus-species">Lactobacillus reuteri</named-content>
title_sort	complex responses to hydrogen peroxide and hypochlorous acid by the probiotic bacterium <named-content content-type="genus-species">lactobacillus reuteri</named-content>
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/8ae048719e8341598290cf3855a23cc3
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Complex Responses to Hydrogen Peroxide and Hypochlorous Acid by the Probiotic Bacterium <named-content content-type="genus-species">Lactobacillus reuteri</named-content>

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