A protein aggregation based test for screening of the agents affecting thermostability of proteins.

To search for agents affecting thermal stability of proteins, a test based on the registration of protein aggregation in the regime of heating with a constant rate was used. The initial parts of the dependences of the light scattering intensity (I) on temperature (T) were analyzed using the followin...

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Autores principales:	Tatyana Eronina, Vera Borzova, Olga Maloletkina, Sergey Kleymenov, Regina Asryants, Kira Markossian, Boris Kurganov
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8ae27d9ab41646138d21c101bfc010c9
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spelling	oai:doaj.org-article:8ae27d9ab41646138d21c101bfc010c92021-11-18T06:50:30ZA protein aggregation based test for screening of the agents affecting thermostability of proteins.1932-620310.1371/journal.pone.0022154https://doaj.org/article/8ae27d9ab41646138d21c101bfc010c92011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21760963/?tool=EBIhttps://doaj.org/toc/1932-6203To search for agents affecting thermal stability of proteins, a test based on the registration of protein aggregation in the regime of heating with a constant rate was used. The initial parts of the dependences of the light scattering intensity (I) on temperature (T) were analyzed using the following empiric equation: I = K(agg)(T-T(0))(2), where K(agg) is the parameter characterizing the initial rate of aggregation and T(0) is a temperature at which the initial increase in the light scattering intensity is registered. The aggregation data are interpreted in the frame of the model assuming the formation of the start aggregates at the initial stages of the aggregation process. Parameter T(0) corresponds to the moment of the origination of the start aggregates. The applicability of the proposed approach was demonstrated on the examples of thermal aggregation of glycogen phosphorylase b from rabbit skeletal muscles and bovine liver glutamate dehydrogenase studied in the presence of agents of different chemical nature. The elaborated approach to the study of protein aggregation may be used for rapid identification of small molecules that interact with protein targets.Tatyana EroninaVera BorzovaOlga MaloletkinaSergey KleymenovRegina AsryantsKira MarkossianBoris KurganovPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e22154 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tatyana Eronina Vera Borzova Olga Maloletkina Sergey Kleymenov Regina Asryants Kira Markossian Boris Kurganov A protein aggregation based test for screening of the agents affecting thermostability of proteins.
description	To search for agents affecting thermal stability of proteins, a test based on the registration of protein aggregation in the regime of heating with a constant rate was used. The initial parts of the dependences of the light scattering intensity (I) on temperature (T) were analyzed using the following empiric equation: I = K(agg)(T-T(0))(2), where K(agg) is the parameter characterizing the initial rate of aggregation and T(0) is a temperature at which the initial increase in the light scattering intensity is registered. The aggregation data are interpreted in the frame of the model assuming the formation of the start aggregates at the initial stages of the aggregation process. Parameter T(0) corresponds to the moment of the origination of the start aggregates. The applicability of the proposed approach was demonstrated on the examples of thermal aggregation of glycogen phosphorylase b from rabbit skeletal muscles and bovine liver glutamate dehydrogenase studied in the presence of agents of different chemical nature. The elaborated approach to the study of protein aggregation may be used for rapid identification of small molecules that interact with protein targets.
format	article
author	Tatyana Eronina Vera Borzova Olga Maloletkina Sergey Kleymenov Regina Asryants Kira Markossian Boris Kurganov
author_facet	Tatyana Eronina Vera Borzova Olga Maloletkina Sergey Kleymenov Regina Asryants Kira Markossian Boris Kurganov
author_sort	Tatyana Eronina
title	A protein aggregation based test for screening of the agents affecting thermostability of proteins.
title_short	A protein aggregation based test for screening of the agents affecting thermostability of proteins.
title_full	A protein aggregation based test for screening of the agents affecting thermostability of proteins.
title_fullStr	A protein aggregation based test for screening of the agents affecting thermostability of proteins.
title_full_unstemmed	A protein aggregation based test for screening of the agents affecting thermostability of proteins.
title_sort	protein aggregation based test for screening of the agents affecting thermostability of proteins.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/8ae27d9ab41646138d21c101bfc010c9
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A protein aggregation based test for screening of the agents affecting thermostability of proteins.

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