Antibiotic loaded β-tricalcium phosphate/calcium sulfate for antimicrobial potency, prevention and killing efficacy of Pseudomonas aeruginosa and Staphylococcus aureus biofilms

Abstract This study investigated the efficacy of a biphasic synthetic β-tricalcium phosphate/calcium sulfate (β-TCP/CS) bone graft substitute for compatibility with vancomycin (V) in combination with tobramycin (T) or gentamicin (G) evidenced by the duration of potency and the prevention and killing...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nan Jiang, Devendra H. Dusane, Jacob R. Brooks, Craig P. Delury, Sean S. Aiken, Phillip A. Laycock, Paul Stoodley
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8ae362f79fc5406b9705b0c0cf9f87d4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8ae362f79fc5406b9705b0c0cf9f87d4
record_format	dspace
spelling	oai:doaj.org-article:8ae362f79fc5406b9705b0c0cf9f87d42021-12-02T14:12:09ZAntibiotic loaded β-tricalcium phosphate/calcium sulfate for antimicrobial potency, prevention and killing efficacy of Pseudomonas aeruginosa and Staphylococcus aureus biofilms10.1038/s41598-020-80764-62045-2322https://doaj.org/article/8ae362f79fc5406b9705b0c0cf9f87d42021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80764-6https://doaj.org/toc/2045-2322Abstract This study investigated the efficacy of a biphasic synthetic β-tricalcium phosphate/calcium sulfate (β-TCP/CS) bone graft substitute for compatibility with vancomycin (V) in combination with tobramycin (T) or gentamicin (G) evidenced by the duration of potency and the prevention and killing efficacies of P. aeruginosa (PAO1) and S. aureus (SAP231) biofilms in in vitro assays. Antibiotic loaded β-TCP/CS beads were compared with antibiotic loaded beads formed from a well characterized synthetic calcium sulfate (CS) bone void filler. β-TCP/CS antibiotic loaded showed antimicrobial potency against PAO1 in a repeated Kirby-Bauer like zone of inhibition assay for 6 days compared to 8 days for CS. However, both bead types showed potency against SAP231 for 40 days. Both formulations loaded with V + T completely prevented biofilm formation (CFU below detection limits) for the 3 days of the experiment with daily fresh inoculum challenges (P < 0.001). In addition, both antibiotic loaded materials and antibiotic combinations significantly reduced the bioburden of pre-grown biofilms by between 3 and 5 logs (P < 0.001) with V + G performing slightly better against PAO1 than V + T. Our data, combined with previous data on osteogenesis suggest that antibiotic loaded β-TCP/CS may have potential to stimulate osteogenesis through acting as a scaffold as well as simultaneously protecting against biofilm infection. Future in vivo experiments and clinical investigations are warranted to more comprehensively evaluate the use of β-TCP/CS in the management of orthopaedic infections.Nan JiangDevendra H. DusaneJacob R. BrooksCraig P. DelurySean S. AikenPhillip A. LaycockPaul StoodleyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nan Jiang Devendra H. Dusane Jacob R. Brooks Craig P. Delury Sean S. Aiken Phillip A. Laycock Paul Stoodley Antibiotic loaded β-tricalcium phosphate/calcium sulfate for antimicrobial potency, prevention and killing efficacy of Pseudomonas aeruginosa and Staphylococcus aureus biofilms
description	Abstract This study investigated the efficacy of a biphasic synthetic β-tricalcium phosphate/calcium sulfate (β-TCP/CS) bone graft substitute for compatibility with vancomycin (V) in combination with tobramycin (T) or gentamicin (G) evidenced by the duration of potency and the prevention and killing efficacies of P. aeruginosa (PAO1) and S. aureus (SAP231) biofilms in in vitro assays. Antibiotic loaded β-TCP/CS beads were compared with antibiotic loaded beads formed from a well characterized synthetic calcium sulfate (CS) bone void filler. β-TCP/CS antibiotic loaded showed antimicrobial potency against PAO1 in a repeated Kirby-Bauer like zone of inhibition assay for 6 days compared to 8 days for CS. However, both bead types showed potency against SAP231 for 40 days. Both formulations loaded with V + T completely prevented biofilm formation (CFU below detection limits) for the 3 days of the experiment with daily fresh inoculum challenges (P < 0.001). In addition, both antibiotic loaded materials and antibiotic combinations significantly reduced the bioburden of pre-grown biofilms by between 3 and 5 logs (P < 0.001) with V + G performing slightly better against PAO1 than V + T. Our data, combined with previous data on osteogenesis suggest that antibiotic loaded β-TCP/CS may have potential to stimulate osteogenesis through acting as a scaffold as well as simultaneously protecting against biofilm infection. Future in vivo experiments and clinical investigations are warranted to more comprehensively evaluate the use of β-TCP/CS in the management of orthopaedic infections.
format	article
author	Nan Jiang Devendra H. Dusane Jacob R. Brooks Craig P. Delury Sean S. Aiken Phillip A. Laycock Paul Stoodley
author_facet	Nan Jiang Devendra H. Dusane Jacob R. Brooks Craig P. Delury Sean S. Aiken Phillip A. Laycock Paul Stoodley
author_sort	Nan Jiang
title	Antibiotic loaded β-tricalcium phosphate/calcium sulfate for antimicrobial potency, prevention and killing efficacy of Pseudomonas aeruginosa and Staphylococcus aureus biofilms
title_short	Antibiotic loaded β-tricalcium phosphate/calcium sulfate for antimicrobial potency, prevention and killing efficacy of Pseudomonas aeruginosa and Staphylococcus aureus biofilms
title_full	Antibiotic loaded β-tricalcium phosphate/calcium sulfate for antimicrobial potency, prevention and killing efficacy of Pseudomonas aeruginosa and Staphylococcus aureus biofilms
title_fullStr	Antibiotic loaded β-tricalcium phosphate/calcium sulfate for antimicrobial potency, prevention and killing efficacy of Pseudomonas aeruginosa and Staphylococcus aureus biofilms
title_full_unstemmed	Antibiotic loaded β-tricalcium phosphate/calcium sulfate for antimicrobial potency, prevention and killing efficacy of Pseudomonas aeruginosa and Staphylococcus aureus biofilms
title_sort	antibiotic loaded β-tricalcium phosphate/calcium sulfate for antimicrobial potency, prevention and killing efficacy of pseudomonas aeruginosa and staphylococcus aureus biofilms
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8ae362f79fc5406b9705b0c0cf9f87d4
work_keys_str_mv	AT nanjiang antibioticloadedbtricalciumphosphatecalciumsulfateforantimicrobialpotencypreventionandkillingefficacyofpseudomonasaeruginosaandstaphylococcusaureusbiofilms AT devendrahdusane antibioticloadedbtricalciumphosphatecalciumsulfateforantimicrobialpotencypreventionandkillingefficacyofpseudomonasaeruginosaandstaphylococcusaureusbiofilms AT jacobrbrooks antibioticloadedbtricalciumphosphatecalciumsulfateforantimicrobialpotencypreventionandkillingefficacyofpseudomonasaeruginosaandstaphylococcusaureusbiofilms AT craigpdelury antibioticloadedbtricalciumphosphatecalciumsulfateforantimicrobialpotencypreventionandkillingefficacyofpseudomonasaeruginosaandstaphylococcusaureusbiofilms AT seansaiken antibioticloadedbtricalciumphosphatecalciumsulfateforantimicrobialpotencypreventionandkillingefficacyofpseudomonasaeruginosaandstaphylococcusaureusbiofilms AT phillipalaycock antibioticloadedbtricalciumphosphatecalciumsulfateforantimicrobialpotencypreventionandkillingefficacyofpseudomonasaeruginosaandstaphylococcusaureusbiofilms AT paulstoodley antibioticloadedbtricalciumphosphatecalciumsulfateforantimicrobialpotencypreventionandkillingefficacyofpseudomonasaeruginosaandstaphylococcusaureusbiofilms
_version_	1718391796179927040

Antibiotic loaded β-tricalcium phosphate/calcium sulfate for antimicrobial potency, prevention and killing efficacy of Pseudomonas aeruginosa and Staphylococcus aureus biofilms

Ejemplares similares