Cell-specific thioautotrophic productivity of epsilon-proteobacterial epibionts associated with Shinkaia crosnieri.

In this study, we report experimental evidence of the thioautotrophic activity of the epibiotic microbial community associated with the setae of Shinkaia crosnieri, a galatheid crab that is endemic to deep-sea hydrothermal systems in the Okinawa Trough in Japan. Microbial consumption of reduced sulf...

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Autores principales:	Tomo-o Watsuji, Manabu Nishizawa, Yuki Morono, Hisako Hirayama, Shinsuke Kawagucci, Naoto Takahata, Yuji Sano, Ken Takai
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8ae468f068024aeabeb42c76dc533ce7
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spelling	oai:doaj.org-article:8ae468f068024aeabeb42c76dc533ce72021-11-18T08:13:29ZCell-specific thioautotrophic productivity of epsilon-proteobacterial epibionts associated with Shinkaia crosnieri.1932-620310.1371/journal.pone.0046282https://doaj.org/article/8ae468f068024aeabeb42c76dc533ce72012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23056274/?tool=EBIhttps://doaj.org/toc/1932-6203In this study, we report experimental evidence of the thioautotrophic activity of the epibiotic microbial community associated with the setae of Shinkaia crosnieri, a galatheid crab that is endemic to deep-sea hydrothermal systems in the Okinawa Trough in Japan. Microbial consumption of reduced sulfur compounds under in situ hydrostatic and atmospheric pressure provided evidence of sulfur-oxidizing activity by the epibiotic microbial community; the rate of sulfur oxidation was similar under in situ and decompressed conditions. Results of the microbial consumption of reduced sulfur compounds and tracer experiments using (13)C-labeled bicarbonate in the presence and absence of thiosulfate (used as a thioautotrophic substrate) convincingly demonstrated that the epibiotic microbial community on S. crosnieri drove primary production via an energy metabolism that was coupled with the oxidation of reductive sulfur compounds. A combination of tracer experiments, fluorescence in situ hybridization (FISH) and nano-scale secondary ion mass spectrometry (Nano-SIMS) indicated that the filamentous cells of the genus Sulfurovum belonging to the class Epsilonproteobacteria were thioautotrophs in the epibiotic community of S. crosnieri. In conclusion, our results strongly suggest that thioautotrophic production by Sulfurovum members present as the epibiotic microbial community play a predominant role in a probable nutritional ectosymbiosis with S. crosnieri.Tomo-o WatsujiManabu NishizawaYuki MoronoHisako HirayamaShinsuke KawagucciNaoto TakahataYuji SanoKen TakaiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e46282 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tomo-o Watsuji Manabu Nishizawa Yuki Morono Hisako Hirayama Shinsuke Kawagucci Naoto Takahata Yuji Sano Ken Takai Cell-specific thioautotrophic productivity of epsilon-proteobacterial epibionts associated with Shinkaia crosnieri.
description	In this study, we report experimental evidence of the thioautotrophic activity of the epibiotic microbial community associated with the setae of Shinkaia crosnieri, a galatheid crab that is endemic to deep-sea hydrothermal systems in the Okinawa Trough in Japan. Microbial consumption of reduced sulfur compounds under in situ hydrostatic and atmospheric pressure provided evidence of sulfur-oxidizing activity by the epibiotic microbial community; the rate of sulfur oxidation was similar under in situ and decompressed conditions. Results of the microbial consumption of reduced sulfur compounds and tracer experiments using (13)C-labeled bicarbonate in the presence and absence of thiosulfate (used as a thioautotrophic substrate) convincingly demonstrated that the epibiotic microbial community on S. crosnieri drove primary production via an energy metabolism that was coupled with the oxidation of reductive sulfur compounds. A combination of tracer experiments, fluorescence in situ hybridization (FISH) and nano-scale secondary ion mass spectrometry (Nano-SIMS) indicated that the filamentous cells of the genus Sulfurovum belonging to the class Epsilonproteobacteria were thioautotrophs in the epibiotic community of S. crosnieri. In conclusion, our results strongly suggest that thioautotrophic production by Sulfurovum members present as the epibiotic microbial community play a predominant role in a probable nutritional ectosymbiosis with S. crosnieri.
format	article
author	Tomo-o Watsuji Manabu Nishizawa Yuki Morono Hisako Hirayama Shinsuke Kawagucci Naoto Takahata Yuji Sano Ken Takai
author_facet	Tomo-o Watsuji Manabu Nishizawa Yuki Morono Hisako Hirayama Shinsuke Kawagucci Naoto Takahata Yuji Sano Ken Takai
author_sort	Tomo-o Watsuji
title	Cell-specific thioautotrophic productivity of epsilon-proteobacterial epibionts associated with Shinkaia crosnieri.
title_short	Cell-specific thioautotrophic productivity of epsilon-proteobacterial epibionts associated with Shinkaia crosnieri.
title_full	Cell-specific thioautotrophic productivity of epsilon-proteobacterial epibionts associated with Shinkaia crosnieri.
title_fullStr	Cell-specific thioautotrophic productivity of epsilon-proteobacterial epibionts associated with Shinkaia crosnieri.
title_full_unstemmed	Cell-specific thioautotrophic productivity of epsilon-proteobacterial epibionts associated with Shinkaia crosnieri.
title_sort	cell-specific thioautotrophic productivity of epsilon-proteobacterial epibionts associated with shinkaia crosnieri.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/8ae468f068024aeabeb42c76dc533ce7
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Cell-specific thioautotrophic productivity of epsilon-proteobacterial epibionts associated with Shinkaia crosnieri.

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