Correlation of cellular traction forces and dissociation kinetics of adhesive protein zyxin revealed by multi-parametric live cell microscopy.

Cells exert traction forces on the extracellular matrix to which they are adhered through the formation of focal adhesions. Spatial-temporal regulation of traction forces is crucial in cell adhesion, migration, cellular division, and remodeling of the extracellular matrix. By cultivating cells on po...

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Autores principales:	Lorena Sigaut, Micaela Bianchi, Catalina von Bilderling, Lía Isabel Pietrasanta
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8ae72044821e4f37af5898dca7e40e65
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spelling	oai:doaj.org-article:8ae72044821e4f37af5898dca7e40e652021-12-02T20:04:08ZCorrelation of cellular traction forces and dissociation kinetics of adhesive protein zyxin revealed by multi-parametric live cell microscopy.1932-620310.1371/journal.pone.0251411https://doaj.org/article/8ae72044821e4f37af5898dca7e40e652021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251411https://doaj.org/toc/1932-6203Cells exert traction forces on the extracellular matrix to which they are adhered through the formation of focal adhesions. Spatial-temporal regulation of traction forces is crucial in cell adhesion, migration, cellular division, and remodeling of the extracellular matrix. By cultivating cells on polyacrylamide hydrogels of different stiffness we were able to investigate the effects of substrate stiffness on the generation of cellular traction forces by Traction Force Microscopy (TFM), and characterize the molecular dynamics of the focal adhesion protein zyxin by Fluorescence Correlation Spectroscopy (FCS) and Fluorescence Recovery After Photobleaching (FRAP). As the rigidity of the substrate increases, we observed an increment of both, cellular traction generation and zyxin residence time at the focal adhesions, while its diffusion would not be altered. Moreover, we found a positive correlation between the traction forces exerted by cells and the residence time of zyxin at the substrate elasticities studied. We found that this correlation persists at the subcellular level, even if there is no variation in substrate stiffness, revealing that focal adhesions that exert greater traction present longer residence time for zyxin, i.e., zyxin protein has less probability to dissociate from the focal adhesion.Lorena SigautMicaela BianchiCatalina von BilderlingLía Isabel PietrasantaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0251411 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lorena Sigaut Micaela Bianchi Catalina von Bilderling Lía Isabel Pietrasanta Correlation of cellular traction forces and dissociation kinetics of adhesive protein zyxin revealed by multi-parametric live cell microscopy.
description	Cells exert traction forces on the extracellular matrix to which they are adhered through the formation of focal adhesions. Spatial-temporal regulation of traction forces is crucial in cell adhesion, migration, cellular division, and remodeling of the extracellular matrix. By cultivating cells on polyacrylamide hydrogels of different stiffness we were able to investigate the effects of substrate stiffness on the generation of cellular traction forces by Traction Force Microscopy (TFM), and characterize the molecular dynamics of the focal adhesion protein zyxin by Fluorescence Correlation Spectroscopy (FCS) and Fluorescence Recovery After Photobleaching (FRAP). As the rigidity of the substrate increases, we observed an increment of both, cellular traction generation and zyxin residence time at the focal adhesions, while its diffusion would not be altered. Moreover, we found a positive correlation between the traction forces exerted by cells and the residence time of zyxin at the substrate elasticities studied. We found that this correlation persists at the subcellular level, even if there is no variation in substrate stiffness, revealing that focal adhesions that exert greater traction present longer residence time for zyxin, i.e., zyxin protein has less probability to dissociate from the focal adhesion.
format	article
author	Lorena Sigaut Micaela Bianchi Catalina von Bilderling Lía Isabel Pietrasanta
author_facet	Lorena Sigaut Micaela Bianchi Catalina von Bilderling Lía Isabel Pietrasanta
author_sort	Lorena Sigaut
title	Correlation of cellular traction forces and dissociation kinetics of adhesive protein zyxin revealed by multi-parametric live cell microscopy.
title_short	Correlation of cellular traction forces and dissociation kinetics of adhesive protein zyxin revealed by multi-parametric live cell microscopy.
title_full	Correlation of cellular traction forces and dissociation kinetics of adhesive protein zyxin revealed by multi-parametric live cell microscopy.
title_fullStr	Correlation of cellular traction forces and dissociation kinetics of adhesive protein zyxin revealed by multi-parametric live cell microscopy.
title_full_unstemmed	Correlation of cellular traction forces and dissociation kinetics of adhesive protein zyxin revealed by multi-parametric live cell microscopy.
title_sort	correlation of cellular traction forces and dissociation kinetics of adhesive protein zyxin revealed by multi-parametric live cell microscopy.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/8ae72044821e4f37af5898dca7e40e65
work_keys_str_mv	AT lorenasigaut correlationofcellulartractionforcesanddissociationkineticsofadhesiveproteinzyxinrevealedbymultiparametriclivecellmicroscopy AT micaelabianchi correlationofcellulartractionforcesanddissociationkineticsofadhesiveproteinzyxinrevealedbymultiparametriclivecellmicroscopy AT catalinavonbilderling correlationofcellulartractionforcesanddissociationkineticsofadhesiveproteinzyxinrevealedbymultiparametriclivecellmicroscopy AT liaisabelpietrasanta correlationofcellulartractionforcesanddissociationkineticsofadhesiveproteinzyxinrevealedbymultiparametriclivecellmicroscopy
_version_	1718375614960893952

Correlation of cellular traction forces and dissociation kinetics of adhesive protein zyxin revealed by multi-parametric live cell microscopy.

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