Identification of mucin degraders of the human gut microbiota

Abstract Mucins are large glycoproteins consisting of approximately 80% of hetero-oligosaccharides. Gut mucin degraders of healthy subjects were investigated, through a culture dependent and independent approach. The faeces of five healthy adults were subjected to three steps of anaerobic enrichment...

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Autores principales:	Stefano Raimondi, Eliana Musmeci, Francesco Candeliere, Alberto Amaretti, Maddalena Rossi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8ae789968b694c0c9f2c69079b294d41
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spelling	oai:doaj.org-article:8ae789968b694c0c9f2c69079b294d412021-12-02T15:49:53ZIdentification of mucin degraders of the human gut microbiota10.1038/s41598-021-90553-42045-2322https://doaj.org/article/8ae789968b694c0c9f2c69079b294d412021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90553-4https://doaj.org/toc/2045-2322Abstract Mucins are large glycoproteins consisting of approximately 80% of hetero-oligosaccharides. Gut mucin degraders of healthy subjects were investigated, through a culture dependent and independent approach. The faeces of five healthy adults were subjected to three steps of anaerobic enrichment in a medium with sole mucins as carbon and nitrogen sources. The bacterial community was compared before and after the enrichment by 16S rRNA gene profiling. Bacteria capable of fermenting sugars, such as Anaerotruncus, Holdemania, and Enterococcaceae likely took advantage of the carbohydrate chains. Escherichia coli and Enterobacteriaceae, Peptococcales, the Coriobacteriale Eggerthella, and a variety of Clostridia such as Oscillospiraceae, Anaerotruncus, and Lachnoclostridium, significantly increased and likely participated to the degradation of the protein backbone of mucin. The affinity of E. coli and Enterobacteriaceae for mucin may facilitate the access to the gut mucosa, promoting gut barrier damage and triggering systemic inflammatory responses. Only three species of strict anaerobes able to grow on mucin were isolated from the enrichments of five different microbiota: Clostridium disporicum, Clostridium tertium, and Paraclostridium benzoelyticum. The limited number of species isolated confirms that in the gut the degradation of these glycoproteins results from cooperation and cross-feeding among several species exhibiting different metabolic capabilities.Stefano RaimondiEliana MusmeciFrancesco CandeliereAlberto AmarettiMaddalena RossiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stefano Raimondi Eliana Musmeci Francesco Candeliere Alberto Amaretti Maddalena Rossi Identification of mucin degraders of the human gut microbiota
description	Abstract Mucins are large glycoproteins consisting of approximately 80% of hetero-oligosaccharides. Gut mucin degraders of healthy subjects were investigated, through a culture dependent and independent approach. The faeces of five healthy adults were subjected to three steps of anaerobic enrichment in a medium with sole mucins as carbon and nitrogen sources. The bacterial community was compared before and after the enrichment by 16S rRNA gene profiling. Bacteria capable of fermenting sugars, such as Anaerotruncus, Holdemania, and Enterococcaceae likely took advantage of the carbohydrate chains. Escherichia coli and Enterobacteriaceae, Peptococcales, the Coriobacteriale Eggerthella, and a variety of Clostridia such as Oscillospiraceae, Anaerotruncus, and Lachnoclostridium, significantly increased and likely participated to the degradation of the protein backbone of mucin. The affinity of E. coli and Enterobacteriaceae for mucin may facilitate the access to the gut mucosa, promoting gut barrier damage and triggering systemic inflammatory responses. Only three species of strict anaerobes able to grow on mucin were isolated from the enrichments of five different microbiota: Clostridium disporicum, Clostridium tertium, and Paraclostridium benzoelyticum. The limited number of species isolated confirms that in the gut the degradation of these glycoproteins results from cooperation and cross-feeding among several species exhibiting different metabolic capabilities.
format	article
author	Stefano Raimondi Eliana Musmeci Francesco Candeliere Alberto Amaretti Maddalena Rossi
author_facet	Stefano Raimondi Eliana Musmeci Francesco Candeliere Alberto Amaretti Maddalena Rossi
author_sort	Stefano Raimondi
title	Identification of mucin degraders of the human gut microbiota
title_short	Identification of mucin degraders of the human gut microbiota
title_full	Identification of mucin degraders of the human gut microbiota
title_fullStr	Identification of mucin degraders of the human gut microbiota
title_full_unstemmed	Identification of mucin degraders of the human gut microbiota
title_sort	identification of mucin degraders of the human gut microbiota
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8ae789968b694c0c9f2c69079b294d41
work_keys_str_mv	AT stefanoraimondi identificationofmucindegradersofthehumangutmicrobiota AT elianamusmeci identificationofmucindegradersofthehumangutmicrobiota AT francescocandeliere identificationofmucindegradersofthehumangutmicrobiota AT albertoamaretti identificationofmucindegradersofthehumangutmicrobiota AT maddalenarossi identificationofmucindegradersofthehumangutmicrobiota
_version_	1718385703749943296

Identification of mucin degraders of the human gut microbiota

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