The fabrication of an ICA-SF/PLCL nanofibrous membrane by coaxial electrospinning and its effect on bone regeneration in vitro and in vivo

Abstract GBR is currently accepted as one of the most effective approaches for bone defect regeneration relating to dental implant. Icariin is the main active ingredient in the extraction of total flavonoids from the Chinese traditional herb Epimediumbrevicornum Maxim. In this study, ICA was success...

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Autores principales:	Lihua Yin, Kaijuan Wang, Xiaoqin Lv, Rui Sun, Shaohua Yang, Yujie Yang, Yanyun Liu, Jiatao Liu, Jing Zhou, Zhanhai Yu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8ae8d959269e48edb49ba6c51d46ddb8
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spelling	oai:doaj.org-article:8ae8d959269e48edb49ba6c51d46ddb82021-12-02T11:52:27ZThe fabrication of an ICA-SF/PLCL nanofibrous membrane by coaxial electrospinning and its effect on bone regeneration in vitro and in vivo10.1038/s41598-017-07759-82045-2322https://doaj.org/article/8ae8d959269e48edb49ba6c51d46ddb82017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07759-8https://doaj.org/toc/2045-2322Abstract GBR is currently accepted as one of the most effective approaches for bone defect regeneration relating to dental implant. Icariin is the main active ingredient in the extraction of total flavonoids from the Chinese traditional herb Epimediumbrevicornum Maxim. In this study, ICA was successfully incorporated into the nanofibers barrier membrane (ICA-SF/PLCL) as osteoinduction factor by coaxial electrospinning and was released in a sustained and controlled manner. The entire release period included two stages: an initial burst stage (47.54 ± 0.06% on 5 d) and a decreasing and constant stage (82.09 ± 1.86% on 30 d). The membrane has good biocompatibility with BMMSCs anchored and significantly promoted its osteogenic activity. Moreover, in vivo experiment, bone defect covered by ICA-SF/PLCL membrane in rat cranium were statistically repaired compare to other groups. 12 weeks after implantation, in the test group, the new bone formation spread to cover most of the defect region with volume and density of approximately 15.95 ± 3.58 mm3 and 14.02 ± 0.93%. These results demonstrated that ICA-SF/PLCL nanofibrous membrane could be a promising barrier applicated for GBR.Lihua YinKaijuan WangXiaoqin LvRui SunShaohua YangYujie YangYanyun LiuJiatao LiuJing ZhouZhanhai YuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lihua Yin Kaijuan Wang Xiaoqin Lv Rui Sun Shaohua Yang Yujie Yang Yanyun Liu Jiatao Liu Jing Zhou Zhanhai Yu The fabrication of an ICA-SF/PLCL nanofibrous membrane by coaxial electrospinning and its effect on bone regeneration in vitro and in vivo
description	Abstract GBR is currently accepted as one of the most effective approaches for bone defect regeneration relating to dental implant. Icariin is the main active ingredient in the extraction of total flavonoids from the Chinese traditional herb Epimediumbrevicornum Maxim. In this study, ICA was successfully incorporated into the nanofibers barrier membrane (ICA-SF/PLCL) as osteoinduction factor by coaxial electrospinning and was released in a sustained and controlled manner. The entire release period included two stages: an initial burst stage (47.54 ± 0.06% on 5 d) and a decreasing and constant stage (82.09 ± 1.86% on 30 d). The membrane has good biocompatibility with BMMSCs anchored and significantly promoted its osteogenic activity. Moreover, in vivo experiment, bone defect covered by ICA-SF/PLCL membrane in rat cranium were statistically repaired compare to other groups. 12 weeks after implantation, in the test group, the new bone formation spread to cover most of the defect region with volume and density of approximately 15.95 ± 3.58 mm3 and 14.02 ± 0.93%. These results demonstrated that ICA-SF/PLCL nanofibrous membrane could be a promising barrier applicated for GBR.
format	article
author	Lihua Yin Kaijuan Wang Xiaoqin Lv Rui Sun Shaohua Yang Yujie Yang Yanyun Liu Jiatao Liu Jing Zhou Zhanhai Yu
author_facet	Lihua Yin Kaijuan Wang Xiaoqin Lv Rui Sun Shaohua Yang Yujie Yang Yanyun Liu Jiatao Liu Jing Zhou Zhanhai Yu
author_sort	Lihua Yin
title	The fabrication of an ICA-SF/PLCL nanofibrous membrane by coaxial electrospinning and its effect on bone regeneration in vitro and in vivo
title_short	The fabrication of an ICA-SF/PLCL nanofibrous membrane by coaxial electrospinning and its effect on bone regeneration in vitro and in vivo
title_full	The fabrication of an ICA-SF/PLCL nanofibrous membrane by coaxial electrospinning and its effect on bone regeneration in vitro and in vivo
title_fullStr	The fabrication of an ICA-SF/PLCL nanofibrous membrane by coaxial electrospinning and its effect on bone regeneration in vitro and in vivo
title_full_unstemmed	The fabrication of an ICA-SF/PLCL nanofibrous membrane by coaxial electrospinning and its effect on bone regeneration in vitro and in vivo
title_sort	fabrication of an ica-sf/plcl nanofibrous membrane by coaxial electrospinning and its effect on bone regeneration in vitro and in vivo
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8ae8d959269e48edb49ba6c51d46ddb8
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The fabrication of an ICA-SF/PLCL nanofibrous membrane by coaxial electrospinning and its effect on bone regeneration in vitro and in vivo

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