Application of SARS-CoV-2 Serology to Address Public Health Priorities

Background: Antibodies against SARS-CoV-2 can be detected by various testing platforms, but a detailed understanding of assay performance is critical.Methods: We developed and validated a simple enzyme-linked immunosorbent assay (ELISA) to detect IgG binding to the receptor-binding domain (RBD) of S...

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Autores principales:	Amy C. Sherman, Teresa Smith, Yerun Zhu, Kaitlin Taibl, Jessica Howard-Anderson, Taylor Landay, Nora Pisanic, Jennifer Kleinhenz, Trevor W. Simon, Daniel Espinoza, Neena Edupuganti, Skyler Hammond, Nadine Rouphael, Huifeng Shen, Jessica K. Fairley, Srilatha Edupuganti, Jaime A. Cardona-Ospina, Alfonso J. Rodriguez-Morales, Lakshmanane Premkumar, Jens Wrammert, Rick Tarleton, Scott Fridkin, Christopher D. Heaney, Erin M. Scherer, Matthew H. Collins
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	SARS-CoV-2 ELISA antibody response serology public health Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/8ae9ca0164134c2c89f0f82d83927843
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spelling	oai:doaj.org-article:8ae9ca0164134c2c89f0f82d839278432021-11-30T12:48:28ZApplication of SARS-CoV-2 Serology to Address Public Health Priorities2296-256510.3389/fpubh.2021.744535https://doaj.org/article/8ae9ca0164134c2c89f0f82d839278432021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpubh.2021.744535/fullhttps://doaj.org/toc/2296-2565Background: Antibodies against SARS-CoV-2 can be detected by various testing platforms, but a detailed understanding of assay performance is critical.Methods: We developed and validated a simple enzyme-linked immunosorbent assay (ELISA) to detect IgG binding to the receptor-binding domain (RBD) of SARS-CoV-2, which was then applied for surveillance. ELISA results were compared to a set of complimentary serologic assays using a large panel of clinical research samples.Results: The RBD ELISA exhibited robust performance in ROC curve analysis (AUC> 0.99; Se = 89%, Sp = 99.3%). Antibodies were detected in 23/353 (6.5%) healthcare workers, 6/9 RT-PCR-confirmed mild COVID-19 cases, and 0/30 non-COVID-19 cases from an ambulatory site. RBD ELISA showed a positive correlation with neutralizing activity (p = <0.0001, R2 = 0.26).Conclusions: We applied a validated SARS-CoV-2-specific IgG ELISA in multiple contexts and performed orthogonal testing on samples. This study demonstrates the utility of a simple serologic assay for detecting prior SARS-CoV-2 infection, particularly as a tool for efficiently testing large numbers of samples as in population surveillance. Our work also highlights that precise understanding of SARS-CoV-2 infection and immunity at the individual level, particularly with wide availability of vaccination, may be improved by orthogonal testing and/or more complex assays such as multiplex bead assays.Amy C. ShermanAmy C. ShermanTeresa SmithYerun ZhuKaitlin TaiblJessica Howard-AndersonTaylor LandayNora PisanicJennifer KleinhenzJennifer KleinhenzTrevor W. SimonDaniel EspinozaNeena EdupugantiSkyler HammondNadine RouphaelHuifeng ShenJessica K. FairleySrilatha EdupugantiJaime A. Cardona-OspinaJaime A. Cardona-OspinaAlfonso J. Rodriguez-MoralesAlfonso J. Rodriguez-MoralesLakshmanane PremkumarJens WrammertRick TarletonScott FridkinScott FridkinChristopher D. HeaneyErin M. SchererMatthew H. CollinsFrontiers Media S.A.articleSARS-CoV-2ELISAantibody responseserologypublic healthPublic aspects of medicineRA1-1270ENFrontiers in Public Health, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SARS-CoV-2 ELISA antibody response serology public health Public aspects of medicine RA1-1270
spellingShingle	SARS-CoV-2 ELISA antibody response serology public health Public aspects of medicine RA1-1270 Amy C. Sherman Amy C. Sherman Teresa Smith Yerun Zhu Kaitlin Taibl Jessica Howard-Anderson Taylor Landay Nora Pisanic Jennifer Kleinhenz Jennifer Kleinhenz Trevor W. Simon Daniel Espinoza Neena Edupuganti Skyler Hammond Nadine Rouphael Huifeng Shen Jessica K. Fairley Srilatha Edupuganti Jaime A. Cardona-Ospina Jaime A. Cardona-Ospina Alfonso J. Rodriguez-Morales Alfonso J. Rodriguez-Morales Lakshmanane Premkumar Jens Wrammert Rick Tarleton Scott Fridkin Scott Fridkin Christopher D. Heaney Erin M. Scherer Matthew H. Collins Application of SARS-CoV-2 Serology to Address Public Health Priorities
description	Background: Antibodies against SARS-CoV-2 can be detected by various testing platforms, but a detailed understanding of assay performance is critical.Methods: We developed and validated a simple enzyme-linked immunosorbent assay (ELISA) to detect IgG binding to the receptor-binding domain (RBD) of SARS-CoV-2, which was then applied for surveillance. ELISA results were compared to a set of complimentary serologic assays using a large panel of clinical research samples.Results: The RBD ELISA exhibited robust performance in ROC curve analysis (AUC> 0.99; Se = 89%, Sp = 99.3%). Antibodies were detected in 23/353 (6.5%) healthcare workers, 6/9 RT-PCR-confirmed mild COVID-19 cases, and 0/30 non-COVID-19 cases from an ambulatory site. RBD ELISA showed a positive correlation with neutralizing activity (p = <0.0001, R2 = 0.26).Conclusions: We applied a validated SARS-CoV-2-specific IgG ELISA in multiple contexts and performed orthogonal testing on samples. This study demonstrates the utility of a simple serologic assay for detecting prior SARS-CoV-2 infection, particularly as a tool for efficiently testing large numbers of samples as in population surveillance. Our work also highlights that precise understanding of SARS-CoV-2 infection and immunity at the individual level, particularly with wide availability of vaccination, may be improved by orthogonal testing and/or more complex assays such as multiplex bead assays.
format	article
author	Amy C. Sherman Amy C. Sherman Teresa Smith Yerun Zhu Kaitlin Taibl Jessica Howard-Anderson Taylor Landay Nora Pisanic Jennifer Kleinhenz Jennifer Kleinhenz Trevor W. Simon Daniel Espinoza Neena Edupuganti Skyler Hammond Nadine Rouphael Huifeng Shen Jessica K. Fairley Srilatha Edupuganti Jaime A. Cardona-Ospina Jaime A. Cardona-Ospina Alfonso J. Rodriguez-Morales Alfonso J. Rodriguez-Morales Lakshmanane Premkumar Jens Wrammert Rick Tarleton Scott Fridkin Scott Fridkin Christopher D. Heaney Erin M. Scherer Matthew H. Collins
author_facet	Amy C. Sherman Amy C. Sherman Teresa Smith Yerun Zhu Kaitlin Taibl Jessica Howard-Anderson Taylor Landay Nora Pisanic Jennifer Kleinhenz Jennifer Kleinhenz Trevor W. Simon Daniel Espinoza Neena Edupuganti Skyler Hammond Nadine Rouphael Huifeng Shen Jessica K. Fairley Srilatha Edupuganti Jaime A. Cardona-Ospina Jaime A. Cardona-Ospina Alfonso J. Rodriguez-Morales Alfonso J. Rodriguez-Morales Lakshmanane Premkumar Jens Wrammert Rick Tarleton Scott Fridkin Scott Fridkin Christopher D. Heaney Erin M. Scherer Matthew H. Collins
author_sort	Amy C. Sherman
title	Application of SARS-CoV-2 Serology to Address Public Health Priorities
title_short	Application of SARS-CoV-2 Serology to Address Public Health Priorities
title_full	Application of SARS-CoV-2 Serology to Address Public Health Priorities
title_fullStr	Application of SARS-CoV-2 Serology to Address Public Health Priorities
title_full_unstemmed	Application of SARS-CoV-2 Serology to Address Public Health Priorities
title_sort	application of sars-cov-2 serology to address public health priorities
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/8ae9ca0164134c2c89f0f82d83927843
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Application of SARS-CoV-2 Serology to Address Public Health Priorities

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