Simultaneous Determination of Ciprofloxacin and Ofloxacin in Animal Tissues with the Use of Capillary Electrophoresis with Transient Pseudo-Isotachophoresis

We have developed a precise and accurate method for the determination of ciprofloxacin and ofloxacin in meat tissues. Our method utilizes capillary electrophoresis with a transient pseudo-isotachophoresis mechanism and liquid–liquid extraction during sample preparation. For our experiment, a meat ti...

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Autores principales:	Izabella Kośka, Krystian Purgat, Rafał Głowacki, Paweł Kubalczyk
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	capillary electrophoresis transient pseudo-isotachophoresis extraction antibiotics ciprofloxacin ofloxacin Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/8ae9de6b59cd4b06b6ac73785d7dd501
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spelling	oai:doaj.org-article:8ae9de6b59cd4b06b6ac73785d7dd5012021-11-25T18:28:32ZSimultaneous Determination of Ciprofloxacin and Ofloxacin in Animal Tissues with the Use of Capillary Electrophoresis with Transient Pseudo-Isotachophoresis10.3390/molecules262269311420-3049https://doaj.org/article/8ae9de6b59cd4b06b6ac73785d7dd5012021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/6931https://doaj.org/toc/1420-3049We have developed a precise and accurate method for the determination of ciprofloxacin and ofloxacin in meat tissues. Our method utilizes capillary electrophoresis with a transient pseudo-isotachophoresis mechanism and liquid–liquid extraction during sample preparation. For our experiment, a meat tissue sample was homogenized in pH 7.00 phosphate buffer at a ratio of 1:10 (tissue mass: buffer volume; g/mL). The extraction of each sample was carried out twice for 15 min with 600 µL of a mixture of dichloromethane and acetonitrile at a 2:1 volume ratio. We then conducted the electrophoretic separation at a voltage of 16 kV and a temperature of 25 °C using a background electrolyte of 0.1 mol/L phosphate–borate (pH 8.40). We used the UV detection at 288 nm. The experimentally determined LOQs for ciprofloxacin and ofloxacin were 0.27 ppm (0.8 nmol/g tissue) and 0.11 ppm (0.3 nmol/g tissue), respectively. The calibration curves exhibited linearity over the tested concentration range of 2 to 10 nmol/g tissue for both analytes. The relative standard deviation of the determination did not exceed 15%, and the recovery was in the range of 85–115%. We used the method to analyze various meat tissues for their ciprofloxacin and ofloxacin contents.Izabella KośkaKrystian PurgatRafał GłowackiPaweł KubalczykMDPI AGarticlecapillary electrophoresistransient pseudo-isotachophoresisextractionantibioticsciprofloxacinofloxacinOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6931, p 6931 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	capillary electrophoresis transient pseudo-isotachophoresis extraction antibiotics ciprofloxacin ofloxacin Organic chemistry QD241-441
spellingShingle	capillary electrophoresis transient pseudo-isotachophoresis extraction antibiotics ciprofloxacin ofloxacin Organic chemistry QD241-441 Izabella Kośka Krystian Purgat Rafał Głowacki Paweł Kubalczyk Simultaneous Determination of Ciprofloxacin and Ofloxacin in Animal Tissues with the Use of Capillary Electrophoresis with Transient Pseudo-Isotachophoresis
description	We have developed a precise and accurate method for the determination of ciprofloxacin and ofloxacin in meat tissues. Our method utilizes capillary electrophoresis with a transient pseudo-isotachophoresis mechanism and liquid–liquid extraction during sample preparation. For our experiment, a meat tissue sample was homogenized in pH 7.00 phosphate buffer at a ratio of 1:10 (tissue mass: buffer volume; g/mL). The extraction of each sample was carried out twice for 15 min with 600 µL of a mixture of dichloromethane and acetonitrile at a 2:1 volume ratio. We then conducted the electrophoretic separation at a voltage of 16 kV and a temperature of 25 °C using a background electrolyte of 0.1 mol/L phosphate–borate (pH 8.40). We used the UV detection at 288 nm. The experimentally determined LOQs for ciprofloxacin and ofloxacin were 0.27 ppm (0.8 nmol/g tissue) and 0.11 ppm (0.3 nmol/g tissue), respectively. The calibration curves exhibited linearity over the tested concentration range of 2 to 10 nmol/g tissue for both analytes. The relative standard deviation of the determination did not exceed 15%, and the recovery was in the range of 85–115%. We used the method to analyze various meat tissues for their ciprofloxacin and ofloxacin contents.
format	article
author	Izabella Kośka Krystian Purgat Rafał Głowacki Paweł Kubalczyk
author_facet	Izabella Kośka Krystian Purgat Rafał Głowacki Paweł Kubalczyk
author_sort	Izabella Kośka
title	Simultaneous Determination of Ciprofloxacin and Ofloxacin in Animal Tissues with the Use of Capillary Electrophoresis with Transient Pseudo-Isotachophoresis
title_short	Simultaneous Determination of Ciprofloxacin and Ofloxacin in Animal Tissues with the Use of Capillary Electrophoresis with Transient Pseudo-Isotachophoresis
title_full	Simultaneous Determination of Ciprofloxacin and Ofloxacin in Animal Tissues with the Use of Capillary Electrophoresis with Transient Pseudo-Isotachophoresis
title_fullStr	Simultaneous Determination of Ciprofloxacin and Ofloxacin in Animal Tissues with the Use of Capillary Electrophoresis with Transient Pseudo-Isotachophoresis
title_full_unstemmed	Simultaneous Determination of Ciprofloxacin and Ofloxacin in Animal Tissues with the Use of Capillary Electrophoresis with Transient Pseudo-Isotachophoresis
title_sort	simultaneous determination of ciprofloxacin and ofloxacin in animal tissues with the use of capillary electrophoresis with transient pseudo-isotachophoresis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8ae9de6b59cd4b06b6ac73785d7dd501
work_keys_str_mv	AT izabellakoska simultaneousdeterminationofciprofloxacinandofloxacininanimaltissueswiththeuseofcapillaryelectrophoresiswithtransientpseudoisotachophoresis AT krystianpurgat simultaneousdeterminationofciprofloxacinandofloxacininanimaltissueswiththeuseofcapillaryelectrophoresiswithtransientpseudoisotachophoresis AT rafałgłowacki simultaneousdeterminationofciprofloxacinandofloxacininanimaltissueswiththeuseofcapillaryelectrophoresiswithtransientpseudoisotachophoresis AT pawełkubalczyk simultaneousdeterminationofciprofloxacinandofloxacininanimaltissueswiththeuseofcapillaryelectrophoresiswithtransientpseudoisotachophoresis
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Simultaneous Determination of Ciprofloxacin and Ofloxacin in Animal Tissues with the Use of Capillary Electrophoresis with Transient Pseudo-Isotachophoresis

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