Acute kidney injury and inflammatory response of sepsis following cecal ligation and puncture in D-galactose-induced aging rats

Chao Liu,1,* Jie Hu,1,* Zhi Mao,1,* Hongjun Kang,1 Hui Liu,1 Wanlei Fu,2 Yangfan Lv,2 Feihu Zhou1 1Department of Critical Care Medicine, Chinese People’s Liberation Army General Hospital, Beijing, People’s Republic of China; 2Department of Pathology, Xinqiao Hospital, Third Mili...

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Autores principales:	Liu C, Hu J, Mao Z, Kang H, Liu H, Fu W, Lv Y, Zhou F
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Acute kidney injury Inflammatory response Caecal ligation and puncture D-galactose Aging rat Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/8aea683af7f24d93a4abee817ba1b232
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spelling	oai:doaj.org-article:8aea683af7f24d93a4abee817ba1b2322021-12-02T06:55:37ZAcute kidney injury and inflammatory response of sepsis following cecal ligation and puncture in D-galactose-induced aging rats1178-1998https://doaj.org/article/8aea683af7f24d93a4abee817ba1b2322017-03-01T00:00:00Zhttps://www.dovepress.com/acute-kidney-injury-and-inflammatory-response-of-sepsis-following-ceca-peer-reviewed-article-CIAhttps://doaj.org/toc/1178-1998Chao Liu,1,* Jie Hu,1,* Zhi Mao,1,* Hongjun Kang,1 Hui Liu,1 Wanlei Fu,2 Yangfan Lv,2 Feihu Zhou1 1Department of Critical Care Medicine, Chinese People’s Liberation Army General Hospital, Beijing, People’s Republic of China; 2Department of Pathology, Xinqiao Hospital, Third Military Medical University, Chongqing, People’s Republic of China *These authors contributed equally to this work Background: Recently, the D-galactose (D-gal)-induced mimetic aging rat model has been widely used in studies of age-associated diseases, which have shown that chronic D-gal exposure induces premature aging similar to natural aging in rats. With the increasing rate of sepsis in the geriatric population, an easy-access animal model for preclinical studies of elderly sepsis is urgently needed. This study investigates whether a sepsis model that is established in D-gal-induced aging rats can serve as a suitable model for preclinical studies of elderly patients with sepsis.Objective: To investigate the acute kidney injury (AKI) and inflammatory response of sepsis following cecal ligation and puncture (CLP) in D-gal-induced aging rats.Methods: Twelve-week-old male Sprague Dawley rats were divided into low-dose D-gal (L D-gal, 125 mg/kg/d), high-dose D-gal (H D-gal, 500 mg/kg/d), and control groups. After daily subcutaneous injection of D-gal for 6 weeks, the CLP method was used to establish a sepsis model.Results: The mortality was 73.3%, 40%, and 33.3% in the H D-gal, L D-gal, and control groups, respectively. Blood urea nitrogen, creatinine, plasma neutrophil gelatinase-associated lipocalin, interleukin-6, interleukin-10, and tumor necrosis factor-α were markedly increased in the H D-gal group after establishment of the sepsis model (H D-gal vs control, P<0.05 at 12 h and 24 h post-CLP). The rate of severe AKI (RIFLE-F) at 24 h post-CLP was 43% for both the control and L D-gal groups and 80% for the H D-gal group.Conclusion: High-dose-D-gal-induced aging rats are more likely to die from sepsis than are young rats, and probably this is associated with increased severity of septic AKI and an increased inflammatory response. Therefore, use of the high-dose-D-gal-induced aging rat model of sepsis for preclinical studies can provide more useful information for the treatment of sepsis in elderly patients. Keywords: acute kidney injury, inflammatory response, cecal ligation and puncture, D-galactose, aging ratLiu CHu JMao ZKang HLiu HFu WLv YZhou FDove Medical PressarticleAcute kidney injuryInflammatory responseCaecal ligation and punctureD-galactoseAging ratGeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 12, Pp 593-602 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Acute kidney injury Inflammatory response Caecal ligation and puncture D-galactose Aging rat Geriatrics RC952-954.6
spellingShingle	Acute kidney injury Inflammatory response Caecal ligation and puncture D-galactose Aging rat Geriatrics RC952-954.6 Liu C Hu J Mao Z Kang H Liu H Fu W Lv Y Zhou F Acute kidney injury and inflammatory response of sepsis following cecal ligation and puncture in D-galactose-induced aging rats
description	Chao Liu,1,* Jie Hu,1,* Zhi Mao,1,* Hongjun Kang,1 Hui Liu,1 Wanlei Fu,2 Yangfan Lv,2 Feihu Zhou1 1Department of Critical Care Medicine, Chinese People’s Liberation Army General Hospital, Beijing, People’s Republic of China; 2Department of Pathology, Xinqiao Hospital, Third Military Medical University, Chongqing, People’s Republic of China *These authors contributed equally to this work Background: Recently, the D-galactose (D-gal)-induced mimetic aging rat model has been widely used in studies of age-associated diseases, which have shown that chronic D-gal exposure induces premature aging similar to natural aging in rats. With the increasing rate of sepsis in the geriatric population, an easy-access animal model for preclinical studies of elderly sepsis is urgently needed. This study investigates whether a sepsis model that is established in D-gal-induced aging rats can serve as a suitable model for preclinical studies of elderly patients with sepsis.Objective: To investigate the acute kidney injury (AKI) and inflammatory response of sepsis following cecal ligation and puncture (CLP) in D-gal-induced aging rats.Methods: Twelve-week-old male Sprague Dawley rats were divided into low-dose D-gal (L D-gal, 125 mg/kg/d), high-dose D-gal (H D-gal, 500 mg/kg/d), and control groups. After daily subcutaneous injection of D-gal for 6 weeks, the CLP method was used to establish a sepsis model.Results: The mortality was 73.3%, 40%, and 33.3% in the H D-gal, L D-gal, and control groups, respectively. Blood urea nitrogen, creatinine, plasma neutrophil gelatinase-associated lipocalin, interleukin-6, interleukin-10, and tumor necrosis factor-α were markedly increased in the H D-gal group after establishment of the sepsis model (H D-gal vs control, P<0.05 at 12 h and 24 h post-CLP). The rate of severe AKI (RIFLE-F) at 24 h post-CLP was 43% for both the control and L D-gal groups and 80% for the H D-gal group.Conclusion: High-dose-D-gal-induced aging rats are more likely to die from sepsis than are young rats, and probably this is associated with increased severity of septic AKI and an increased inflammatory response. Therefore, use of the high-dose-D-gal-induced aging rat model of sepsis for preclinical studies can provide more useful information for the treatment of sepsis in elderly patients. Keywords: acute kidney injury, inflammatory response, cecal ligation and puncture, D-galactose, aging rat
format	article
author	Liu C Hu J Mao Z Kang H Liu H Fu W Lv Y Zhou F
author_facet	Liu C Hu J Mao Z Kang H Liu H Fu W Lv Y Zhou F
author_sort	Liu C
title	Acute kidney injury and inflammatory response of sepsis following cecal ligation and puncture in D-galactose-induced aging rats
title_short	Acute kidney injury and inflammatory response of sepsis following cecal ligation and puncture in D-galactose-induced aging rats
title_full	Acute kidney injury and inflammatory response of sepsis following cecal ligation and puncture in D-galactose-induced aging rats
title_fullStr	Acute kidney injury and inflammatory response of sepsis following cecal ligation and puncture in D-galactose-induced aging rats
title_full_unstemmed	Acute kidney injury and inflammatory response of sepsis following cecal ligation and puncture in D-galactose-induced aging rats
title_sort	acute kidney injury and inflammatory response of sepsis following cecal ligation and puncture in d-galactose-induced aging rats
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/8aea683af7f24d93a4abee817ba1b232
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Acute kidney injury and inflammatory response of sepsis following cecal ligation and puncture in D-galactose-induced aging rats

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