Characterization of cellular transcriptomic signatures induced by different respiratory viruses in human reconstituted airway epithelia

Abstract Acute respiratory infections, a large part being of viral origin, constitute a major public health issue. To propose alternative and/or new therapeutic approaches, it is necessary to increase our knowledge about the interactions between respiratory viruses and their primary cellular targets...

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Autores principales:	Claire Nicolas de Lamballerie, Andrés Pizzorno, Julia Dubois, Thomas Julien, Blandine Padey, Mendy Bouveret, Aurélien Traversier, Catherine Legras-Lachuer, Bruno Lina, Guy Boivin, Olivier Terrier, Manuel Rosa-Calatrava
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8af0545f4c5a4b2f98c545996b8d0670
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spelling	oai:doaj.org-article:8af0545f4c5a4b2f98c545996b8d06702021-12-02T15:09:45ZCharacterization of cellular transcriptomic signatures induced by different respiratory viruses in human reconstituted airway epithelia10.1038/s41598-019-48013-72045-2322https://doaj.org/article/8af0545f4c5a4b2f98c545996b8d06702019-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-48013-7https://doaj.org/toc/2045-2322Abstract Acute respiratory infections, a large part being of viral origin, constitute a major public health issue. To propose alternative and/or new therapeutic approaches, it is necessary to increase our knowledge about the interactions between respiratory viruses and their primary cellular targets using the most biologically relevant experimental models. In this study, we used RNAseq to characterize and compare the transcriptomic signature of infection induced by different major respiratory viruses (Influenza viruses, hRSV and hMPV) in a model of reconstituted human airway epithelia. Our results confirm the importance of several cellular pathways commonly or specifically induced by these respiratory viruses, such as the innate immune response or antiviral defense. A very interesting common feature revealed by the global virogenomic signature shared between hRSV, hMPV and influenza viruses is the global downregulation of cilium-related gene expression, in good agreement with experimental evaluation of mucociliary clearance. Beyond providing new information about respiratory virus/host interactions, our study also underlines the interest of using biologically relevant experimental models to study human respiratory viruses.Claire Nicolas de LamballerieAndrés PizzornoJulia DuboisThomas JulienBlandine PadeyMendy BouveretAurélien TraversierCatherine Legras-LachuerBruno LinaGuy BoivinOlivier TerrierManuel Rosa-CalatravaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Claire Nicolas de Lamballerie Andrés Pizzorno Julia Dubois Thomas Julien Blandine Padey Mendy Bouveret Aurélien Traversier Catherine Legras-Lachuer Bruno Lina Guy Boivin Olivier Terrier Manuel Rosa-Calatrava Characterization of cellular transcriptomic signatures induced by different respiratory viruses in human reconstituted airway epithelia
description	Abstract Acute respiratory infections, a large part being of viral origin, constitute a major public health issue. To propose alternative and/or new therapeutic approaches, it is necessary to increase our knowledge about the interactions between respiratory viruses and their primary cellular targets using the most biologically relevant experimental models. In this study, we used RNAseq to characterize and compare the transcriptomic signature of infection induced by different major respiratory viruses (Influenza viruses, hRSV and hMPV) in a model of reconstituted human airway epithelia. Our results confirm the importance of several cellular pathways commonly or specifically induced by these respiratory viruses, such as the innate immune response or antiviral defense. A very interesting common feature revealed by the global virogenomic signature shared between hRSV, hMPV and influenza viruses is the global downregulation of cilium-related gene expression, in good agreement with experimental evaluation of mucociliary clearance. Beyond providing new information about respiratory virus/host interactions, our study also underlines the interest of using biologically relevant experimental models to study human respiratory viruses.
format	article
author	Claire Nicolas de Lamballerie Andrés Pizzorno Julia Dubois Thomas Julien Blandine Padey Mendy Bouveret Aurélien Traversier Catherine Legras-Lachuer Bruno Lina Guy Boivin Olivier Terrier Manuel Rosa-Calatrava
author_facet	Claire Nicolas de Lamballerie Andrés Pizzorno Julia Dubois Thomas Julien Blandine Padey Mendy Bouveret Aurélien Traversier Catherine Legras-Lachuer Bruno Lina Guy Boivin Olivier Terrier Manuel Rosa-Calatrava
author_sort	Claire Nicolas de Lamballerie
title	Characterization of cellular transcriptomic signatures induced by different respiratory viruses in human reconstituted airway epithelia
title_short	Characterization of cellular transcriptomic signatures induced by different respiratory viruses in human reconstituted airway epithelia
title_full	Characterization of cellular transcriptomic signatures induced by different respiratory viruses in human reconstituted airway epithelia
title_fullStr	Characterization of cellular transcriptomic signatures induced by different respiratory viruses in human reconstituted airway epithelia
title_full_unstemmed	Characterization of cellular transcriptomic signatures induced by different respiratory viruses in human reconstituted airway epithelia
title_sort	characterization of cellular transcriptomic signatures induced by different respiratory viruses in human reconstituted airway epithelia
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/8af0545f4c5a4b2f98c545996b8d0670
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Characterization of cellular transcriptomic signatures induced by different respiratory viruses in human reconstituted airway epithelia

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