Emulsified nanoparticles containing inactivated influenza virus and CpG oligodeoxynucleotides critically influences the host immune responses in mice.

<h4>Background</h4>Antigen sparing and cross-protective immunity are regarded as crucial in pandemic influenza vaccine development. Both targets can be achieved by adjuvantation strategy to elicit a robust and broadened immune response. We assessed the immunogenicity of an inactivated H5...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ming-Hsi Huang, Su-Chen Lin, Chia-Hsin Hsiao, Hsin-Ju Chao, Hung-Ren Yang, Chien-Chun Liao, Po-Wei Chuang, Huang-Pi Wu, Chiung-Yi Huang, Chih-Hsiang Leng, Shih-Jen Liu, Hsin-Wei Chen, Ai-Hsiang Chou, Alan Yung-Chih Hu, Pele Chong
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8af2a45793c74b95b348aab891684f90
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8af2a45793c74b95b348aab891684f90
record_format	dspace
spelling	oai:doaj.org-article:8af2a45793c74b95b348aab891684f902021-11-18T06:35:53ZEmulsified nanoparticles containing inactivated influenza virus and CpG oligodeoxynucleotides critically influences the host immune responses in mice.1932-620310.1371/journal.pone.0012279https://doaj.org/article/8af2a45793c74b95b348aab891684f902010-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20808862/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Antigen sparing and cross-protective immunity are regarded as crucial in pandemic influenza vaccine development. Both targets can be achieved by adjuvantation strategy to elicit a robust and broadened immune response. We assessed the immunogenicity of an inactivated H5N1 whole-virion vaccine (A/Vietnam/1194/2004 NIBRG-14, clade 1) formulated with emulsified nanoparticles and investigated whether it can induce cross-clade protecting immunity.<h4>Methodology/principal findings</h4>After formulation with PELC, a proprietary water-in-oil-in-water nanoemulsion comprising of bioresorbable polymer/Span(R)85/squalene, inactivated virus was intramuscularly administered to mice in either one-dose or two-dose schedule. We found that the antigen-specific serum antibody responses elicited after two doses of non-adjuvanted vaccine were lower than those observed after a single dose of adjuvanted vaccine, PELC and the conventional alum adjuvant as well. Moreover, 5 microg HA of PELC-formulated inactivated virus were capable of inducing higher antibodies than those obtained from alum-adjuvanted vaccine. In single-dose study, we found that encapsulating inactivated virus into emulsified PELC nanoparticles could induce better antibody responses than those formulated with PELC-adsorbed vaccine. However, the potency was rather reduced when the inactivated virus and CpG (an immunostimulatory oligodeoxynucleotide containing unmethylated cytosine-guanosine motifs) were co-encapsulated within the emulsion. Finally, the mice who received PELC/CpG(adsorption)-vaccine could easily and quickly reach 100% of seroprotection against a homologous virus strain and effective cross-protection against a heterologous virus strain (A/Whooper swan/Mongolia/244/2005, clade 2.2).<h4>Conclusions/significance</h4>Encapsulating inactivated H5N1 influenza virus and CpG into emulsified nanoparticles critically influences the humoral responses against pandemic influenza. These results demonstrated that the use of PELC could be as antigen-sparing in preparation for a potential shortage of prophylactic vaccines against local infectious diseases, in particular pandemic influenza. Moreover, the cross-clade neutralizing antibody responses data verify the potential of such adjuvanted H5N1 candidate vaccine as an effective tool in pre-pandemic preparedness.Ming-Hsi HuangSu-Chen LinChia-Hsin HsiaoHsin-Ju ChaoHung-Ren YangChien-Chun LiaoPo-Wei ChuangHuang-Pi WuChiung-Yi HuangChih-Hsiang LengShih-Jen LiuHsin-Wei ChenAi-Hsiang ChouAlan Yung-Chih HuPele ChongPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 8, p e12279 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ming-Hsi Huang Su-Chen Lin Chia-Hsin Hsiao Hsin-Ju Chao Hung-Ren Yang Chien-Chun Liao Po-Wei Chuang Huang-Pi Wu Chiung-Yi Huang Chih-Hsiang Leng Shih-Jen Liu Hsin-Wei Chen Ai-Hsiang Chou Alan Yung-Chih Hu Pele Chong Emulsified nanoparticles containing inactivated influenza virus and CpG oligodeoxynucleotides critically influences the host immune responses in mice.
description	<h4>Background</h4>Antigen sparing and cross-protective immunity are regarded as crucial in pandemic influenza vaccine development. Both targets can be achieved by adjuvantation strategy to elicit a robust and broadened immune response. We assessed the immunogenicity of an inactivated H5N1 whole-virion vaccine (A/Vietnam/1194/2004 NIBRG-14, clade 1) formulated with emulsified nanoparticles and investigated whether it can induce cross-clade protecting immunity.<h4>Methodology/principal findings</h4>After formulation with PELC, a proprietary water-in-oil-in-water nanoemulsion comprising of bioresorbable polymer/Span(R)85/squalene, inactivated virus was intramuscularly administered to mice in either one-dose or two-dose schedule. We found that the antigen-specific serum antibody responses elicited after two doses of non-adjuvanted vaccine were lower than those observed after a single dose of adjuvanted vaccine, PELC and the conventional alum adjuvant as well. Moreover, 5 microg HA of PELC-formulated inactivated virus were capable of inducing higher antibodies than those obtained from alum-adjuvanted vaccine. In single-dose study, we found that encapsulating inactivated virus into emulsified PELC nanoparticles could induce better antibody responses than those formulated with PELC-adsorbed vaccine. However, the potency was rather reduced when the inactivated virus and CpG (an immunostimulatory oligodeoxynucleotide containing unmethylated cytosine-guanosine motifs) were co-encapsulated within the emulsion. Finally, the mice who received PELC/CpG(adsorption)-vaccine could easily and quickly reach 100% of seroprotection against a homologous virus strain and effective cross-protection against a heterologous virus strain (A/Whooper swan/Mongolia/244/2005, clade 2.2).<h4>Conclusions/significance</h4>Encapsulating inactivated H5N1 influenza virus and CpG into emulsified nanoparticles critically influences the humoral responses against pandemic influenza. These results demonstrated that the use of PELC could be as antigen-sparing in preparation for a potential shortage of prophylactic vaccines against local infectious diseases, in particular pandemic influenza. Moreover, the cross-clade neutralizing antibody responses data verify the potential of such adjuvanted H5N1 candidate vaccine as an effective tool in pre-pandemic preparedness.
format	article
author	Ming-Hsi Huang Su-Chen Lin Chia-Hsin Hsiao Hsin-Ju Chao Hung-Ren Yang Chien-Chun Liao Po-Wei Chuang Huang-Pi Wu Chiung-Yi Huang Chih-Hsiang Leng Shih-Jen Liu Hsin-Wei Chen Ai-Hsiang Chou Alan Yung-Chih Hu Pele Chong
author_facet	Ming-Hsi Huang Su-Chen Lin Chia-Hsin Hsiao Hsin-Ju Chao Hung-Ren Yang Chien-Chun Liao Po-Wei Chuang Huang-Pi Wu Chiung-Yi Huang Chih-Hsiang Leng Shih-Jen Liu Hsin-Wei Chen Ai-Hsiang Chou Alan Yung-Chih Hu Pele Chong
author_sort	Ming-Hsi Huang
title	Emulsified nanoparticles containing inactivated influenza virus and CpG oligodeoxynucleotides critically influences the host immune responses in mice.
title_short	Emulsified nanoparticles containing inactivated influenza virus and CpG oligodeoxynucleotides critically influences the host immune responses in mice.
title_full	Emulsified nanoparticles containing inactivated influenza virus and CpG oligodeoxynucleotides critically influences the host immune responses in mice.
title_fullStr	Emulsified nanoparticles containing inactivated influenza virus and CpG oligodeoxynucleotides critically influences the host immune responses in mice.
title_full_unstemmed	Emulsified nanoparticles containing inactivated influenza virus and CpG oligodeoxynucleotides critically influences the host immune responses in mice.
title_sort	emulsified nanoparticles containing inactivated influenza virus and cpg oligodeoxynucleotides critically influences the host immune responses in mice.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/8af2a45793c74b95b348aab891684f90
work_keys_str_mv	AT minghsihuang emulsifiednanoparticlescontaininginactivatedinfluenzavirusandcpgoligodeoxynucleotidescriticallyinfluencesthehostimmuneresponsesinmice AT suchenlin emulsifiednanoparticlescontaininginactivatedinfluenzavirusandcpgoligodeoxynucleotidescriticallyinfluencesthehostimmuneresponsesinmice AT chiahsinhsiao emulsifiednanoparticlescontaininginactivatedinfluenzavirusandcpgoligodeoxynucleotidescriticallyinfluencesthehostimmuneresponsesinmice AT hsinjuchao emulsifiednanoparticlescontaininginactivatedinfluenzavirusandcpgoligodeoxynucleotidescriticallyinfluencesthehostimmuneresponsesinmice AT hungrenyang emulsifiednanoparticlescontaininginactivatedinfluenzavirusandcpgoligodeoxynucleotidescriticallyinfluencesthehostimmuneresponsesinmice AT chienchunliao emulsifiednanoparticlescontaininginactivatedinfluenzavirusandcpgoligodeoxynucleotidescriticallyinfluencesthehostimmuneresponsesinmice AT poweichuang emulsifiednanoparticlescontaininginactivatedinfluenzavirusandcpgoligodeoxynucleotidescriticallyinfluencesthehostimmuneresponsesinmice AT huangpiwu emulsifiednanoparticlescontaininginactivatedinfluenzavirusandcpgoligodeoxynucleotidescriticallyinfluencesthehostimmuneresponsesinmice AT chiungyihuang emulsifiednanoparticlescontaininginactivatedinfluenzavirusandcpgoligodeoxynucleotidescriticallyinfluencesthehostimmuneresponsesinmice AT chihhsiangleng emulsifiednanoparticlescontaininginactivatedinfluenzavirusandcpgoligodeoxynucleotidescriticallyinfluencesthehostimmuneresponsesinmice AT shihjenliu emulsifiednanoparticlescontaininginactivatedinfluenzavirusandcpgoligodeoxynucleotidescriticallyinfluencesthehostimmuneresponsesinmice AT hsinweichen emulsifiednanoparticlescontaininginactivatedinfluenzavirusandcpgoligodeoxynucleotidescriticallyinfluencesthehostimmuneresponsesinmice AT aihsiangchou emulsifiednanoparticlescontaininginactivatedinfluenzavirusandcpgoligodeoxynucleotidescriticallyinfluencesthehostimmuneresponsesinmice AT alanyungchihhu emulsifiednanoparticlescontaininginactivatedinfluenzavirusandcpgoligodeoxynucleotidescriticallyinfluencesthehostimmuneresponsesinmice AT pelechong emulsifiednanoparticlescontaininginactivatedinfluenzavirusandcpgoligodeoxynucleotidescriticallyinfluencesthehostimmuneresponsesinmice
_version_	1718424427805278208

Emulsified nanoparticles containing inactivated influenza virus and CpG oligodeoxynucleotides critically influences the host immune responses in mice.

Ejemplares similares