EXPRESSION OF T-bet FACTOR IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN BRONCHIAL ASTHMA

Abstract. We studied expression of transcription factor T-bet protein in allergic (ABA) versus non-allergic bronchial asthma (NABA), depending on severity grade and clinical phase of the disease. A Western blotting technique was applied, according to the standard protocol (Amersham), using polyclona...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	V. N. Mineev, L. N. Sorokina
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	bronchial asthma t-bet mononuclear cells Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/8af325dc5bc24bb58d4839bb79a38b93
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8af325dc5bc24bb58d4839bb79a38b93
record_format	dspace
spelling	oai:doaj.org-article:8af325dc5bc24bb58d4839bb79a38b932021-11-18T08:03:40ZEXPRESSION OF T-bet FACTOR IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN BRONCHIAL ASTHMA1563-06252313-741X10.15789/1563-0625-2012-1-2-169-173https://doaj.org/article/8af325dc5bc24bb58d4839bb79a38b932014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/327https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. We studied expression of transcription factor T-bet protein in allergic (ABA) versus non-allergic bronchial asthma (NABA), depending on severity grade and clinical phase of the disease. A Western blotting technique was applied, according to the standard protocol (Amersham), using polyclonal anti-T-bet antibodies at a 1:500 dilution (Santa Cruz Biotechnology, UK). The protein levels were quantified, using β-actin as a reference. It is shown for the first time that T-bet expression in ABA patients, as compared to healthy controls, was reduced with increasing disease severity, but did not correlate with clinical phase of the disease. In NABA patients, no sufficient differences in T-bet expression were revealed, as compared with healthy controls. The work was supported by St.Petersburg governmental grant PD04-4.0-102 (Certificate № ASP604079).V. N. MineevL. N. SorokinaSPb RAACIarticlebronchial asthmat-betmononuclear cellsImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 14, Iss 1-2, Pp 169-173 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	bronchial asthma t-bet mononuclear cells Immunologic diseases. Allergy RC581-607
spellingShingle	bronchial asthma t-bet mononuclear cells Immunologic diseases. Allergy RC581-607 V. N. Mineev L. N. Sorokina EXPRESSION OF T-bet FACTOR IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN BRONCHIAL ASTHMA
description	Abstract. We studied expression of transcription factor T-bet protein in allergic (ABA) versus non-allergic bronchial asthma (NABA), depending on severity grade and clinical phase of the disease. A Western blotting technique was applied, according to the standard protocol (Amersham), using polyclonal anti-T-bet antibodies at a 1:500 dilution (Santa Cruz Biotechnology, UK). The protein levels were quantified, using β-actin as a reference. It is shown for the first time that T-bet expression in ABA patients, as compared to healthy controls, was reduced with increasing disease severity, but did not correlate with clinical phase of the disease. In NABA patients, no sufficient differences in T-bet expression were revealed, as compared with healthy controls. The work was supported by St.Petersburg governmental grant PD04-4.0-102 (Certificate № ASP604079).
format	article
author	V. N. Mineev L. N. Sorokina
author_facet	V. N. Mineev L. N. Sorokina
author_sort	V. N. Mineev
title	EXPRESSION OF T-bet FACTOR IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN BRONCHIAL ASTHMA
title_short	EXPRESSION OF T-bet FACTOR IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN BRONCHIAL ASTHMA
title_full	EXPRESSION OF T-bet FACTOR IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN BRONCHIAL ASTHMA
title_fullStr	EXPRESSION OF T-bet FACTOR IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN BRONCHIAL ASTHMA
title_full_unstemmed	EXPRESSION OF T-bet FACTOR IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN BRONCHIAL ASTHMA
title_sort	expression of t-bet factor in peripheral blood mononuclear cells in bronchial asthma
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/8af325dc5bc24bb58d4839bb79a38b93
work_keys_str_mv	AT vnmineev expressionoftbetfactorinperipheralbloodmononuclearcellsinbronchialasthma AT lnsorokina expressionoftbetfactorinperipheralbloodmononuclearcellsinbronchialasthma
_version_	1718422490887225344

EXPRESSION OF T-bet FACTOR IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN BRONCHIAL ASTHMA

Ejemplares similares