Synthesis, Antifungal Activity, and Cytotoxicity of AgBr-NP@CTMAB Hybrid and Its Application in PMMA

Qiao-Jun Zhang,1,2,* Yue Liu,3,* Wen-Ting Zhang,3 Jing-Jing Huang,2 Hao-Hong Li,3 You-Guang Lu,1 Ming Zheng,2 Da-Li Zheng1 1Fujian Key Laboratory of Oral Diseases, Fujian Biological Materials Engineering and Technology Center of Stomatology, School and Hospital of Stomatology, Fujian Medical Univers...

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Autores principales:	Zhang QJ, Liu Y, Zhang WT, Huang JJ, Li HH, Lu YG, Zheng M, Zheng DL
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	c. albicans agbr-np@ctmab polymethyl methacrylate cytotoxicity biofilm Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8af57759f15c4872b10a0d17053615b7
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spelling	oai:doaj.org-article:8af57759f15c4872b10a0d17053615b72021-12-02T15:39:47ZSynthesis, Antifungal Activity, and Cytotoxicity of AgBr-NP@CTMAB Hybrid and Its Application in PMMA1178-2013https://doaj.org/article/8af57759f15c4872b10a0d17053615b72021-04-01T00:00:00Zhttps://www.dovepress.com/synthesis-antifungal-activity-and-cytotoxicity-of-agbr-npctmab-hybrid--peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Qiao-Jun Zhang,1,2,* Yue Liu,3,* Wen-Ting Zhang,3 Jing-Jing Huang,2 Hao-Hong Li,3 You-Guang Lu,1 Ming Zheng,2 Da-Li Zheng1 1Fujian Key Laboratory of Oral Diseases, Fujian Biological Materials Engineering and Technology Center of Stomatology, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, Fujian, 350004, People’s Republic of China; 2Department of Prosthodontics, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, 350001, People’s Republic of China; 3College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, People’s Republic of China*These authors contributed equally to this workCorrespondence: Da-Li ZhengFujian Key Laboratory of Oral Diseases, School and Hospital of Stomatology, Fujian Medical University, 88 Jiaotong Road, Fuzhou, 350004, People’s Republic of ChinaTel +86-591-83720599Email dalizheng@fjmu.edu.cnMing ZhengDepartment of Prosthodontics, School and Hospital of Stomatology, Fujian Medical University, 246 Yangqiao Middle Road, Fuzhou, 350001, People’s Republic of ChinaTel +86-591-83736431Email zmdentist@163.comObjective: To synthesize and determine the antifungal activity of AgBr-nanoparticles (NP) @CTMAB (cetyltrimethyl-ammonium bromide) against Candida albicans (C. albicans) for use in the field of denture cleaning.Methods: The morphology and structure of AgBr-NP@CTMAB were characterized by IR, UV-Vis, XRD and SEM. The antifungal potential of AgBr-NP@CTMAB against C. albicans was determined by colony formation assay and growth curve analysis. PMMA containing AgBr-NP@CTMAB was prepared, and the long-term antifungal efficacy was analyzed. The effect against C. albicans biofilm was analyzed by SEM and OD600 , and the color changes of the specimens were observed by stereomicroscopy after 1 week of incubation. Cytotoxicity to human oral gingival fibroblasts and oral mucosal epithelial cells was detected by Cell Counting Kit-8 (CCK-8) in vitro.Results: The compound showed a good crystalline phase, the presence of AgBr nanoparticles and the hybridization of CTMAB+ with AgBr-NPs. AgBr-NP@CTMAB showed significant antifungal activity against C. albicans at concentrations of 10 μg/mL and 20 μg/mL. PMMA specimens containing AgBr-NP@CTMAB showed no long-term antifungal effect against C. albicans biofilm. The clearance rate of C. albicans attached to PMMA was 44.73% after soaking in 10 μg/mL AgBr-NP@CTMAB solution for 30 min and 91.35% for 8 h. There was no significant residual cytotoxicity or visual color change after soaking.Significance: AgBr-NP@CTMAB showed promising potential treatment for denture cleaners.Keywords: C. albicans, AgBr-NP@CTMAB, polymethyl methacrylate, cytotoxicity, biofilmZhang QJLiu YZhang WTHuang JJLi HHLu YGZheng MZheng DLDove Medical Pressarticlec. albicansagbr-np@ctmabpolymethyl methacrylatecytotoxicitybiofilmMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 3091-3103 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	c. albicans agbr-np@ctmab polymethyl methacrylate cytotoxicity biofilm Medicine (General) R5-920
spellingShingle	c. albicans agbr-np@ctmab polymethyl methacrylate cytotoxicity biofilm Medicine (General) R5-920 Zhang QJ Liu Y Zhang WT Huang JJ Li HH Lu YG Zheng M Zheng DL Synthesis, Antifungal Activity, and Cytotoxicity of AgBr-NP@CTMAB Hybrid and Its Application in PMMA
description	Qiao-Jun Zhang,1,2,* Yue Liu,3,* Wen-Ting Zhang,3 Jing-Jing Huang,2 Hao-Hong Li,3 You-Guang Lu,1 Ming Zheng,2 Da-Li Zheng1 1Fujian Key Laboratory of Oral Diseases, Fujian Biological Materials Engineering and Technology Center of Stomatology, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, Fujian, 350004, People’s Republic of China; 2Department of Prosthodontics, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, 350001, People’s Republic of China; 3College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, People’s Republic of China*These authors contributed equally to this workCorrespondence: Da-Li ZhengFujian Key Laboratory of Oral Diseases, School and Hospital of Stomatology, Fujian Medical University, 88 Jiaotong Road, Fuzhou, 350004, People’s Republic of ChinaTel +86-591-83720599Email dalizheng@fjmu.edu.cnMing ZhengDepartment of Prosthodontics, School and Hospital of Stomatology, Fujian Medical University, 246 Yangqiao Middle Road, Fuzhou, 350001, People’s Republic of ChinaTel +86-591-83736431Email zmdentist@163.comObjective: To synthesize and determine the antifungal activity of AgBr-nanoparticles (NP) @CTMAB (cetyltrimethyl-ammonium bromide) against Candida albicans (C. albicans) for use in the field of denture cleaning.Methods: The morphology and structure of AgBr-NP@CTMAB were characterized by IR, UV-Vis, XRD and SEM. The antifungal potential of AgBr-NP@CTMAB against C. albicans was determined by colony formation assay and growth curve analysis. PMMA containing AgBr-NP@CTMAB was prepared, and the long-term antifungal efficacy was analyzed. The effect against C. albicans biofilm was analyzed by SEM and OD600 , and the color changes of the specimens were observed by stereomicroscopy after 1 week of incubation. Cytotoxicity to human oral gingival fibroblasts and oral mucosal epithelial cells was detected by Cell Counting Kit-8 (CCK-8) in vitro.Results: The compound showed a good crystalline phase, the presence of AgBr nanoparticles and the hybridization of CTMAB+ with AgBr-NPs. AgBr-NP@CTMAB showed significant antifungal activity against C. albicans at concentrations of 10 μg/mL and 20 μg/mL. PMMA specimens containing AgBr-NP@CTMAB showed no long-term antifungal effect against C. albicans biofilm. The clearance rate of C. albicans attached to PMMA was 44.73% after soaking in 10 μg/mL AgBr-NP@CTMAB solution for 30 min and 91.35% for 8 h. There was no significant residual cytotoxicity or visual color change after soaking.Significance: AgBr-NP@CTMAB showed promising potential treatment for denture cleaners.Keywords: C. albicans, AgBr-NP@CTMAB, polymethyl methacrylate, cytotoxicity, biofilm
format	article
author	Zhang QJ Liu Y Zhang WT Huang JJ Li HH Lu YG Zheng M Zheng DL
author_facet	Zhang QJ Liu Y Zhang WT Huang JJ Li HH Lu YG Zheng M Zheng DL
author_sort	Zhang QJ
title	Synthesis, Antifungal Activity, and Cytotoxicity of AgBr-NP@CTMAB Hybrid and Its Application in PMMA
title_short	Synthesis, Antifungal Activity, and Cytotoxicity of AgBr-NP@CTMAB Hybrid and Its Application in PMMA
title_full	Synthesis, Antifungal Activity, and Cytotoxicity of AgBr-NP@CTMAB Hybrid and Its Application in PMMA
title_fullStr	Synthesis, Antifungal Activity, and Cytotoxicity of AgBr-NP@CTMAB Hybrid and Its Application in PMMA
title_full_unstemmed	Synthesis, Antifungal Activity, and Cytotoxicity of AgBr-NP@CTMAB Hybrid and Its Application in PMMA
title_sort	synthesis, antifungal activity, and cytotoxicity of agbr-np@ctmab hybrid and its application in pmma
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/8af57759f15c4872b10a0d17053615b7
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Synthesis, Antifungal Activity, and Cytotoxicity of AgBr-NP@CTMAB Hybrid and Its Application in PMMA

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