Daidzin inhibits growth and induces apoptosis through the JAK2/STAT3 in human cervical cancer HeLa cells

Daidzin, 4′, 7-dihydroxyisoflavone is an isoflavonic phytoestrogen present in leguminous plants. Traditional Chinese medicine utilizes daidzin to treat various diseases such diarrhea, fever, hepatitis, cardiac problems etc. In current study we examined the anticancer activity of daidzin against huma...

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Autores principales:	Zhilin Yao, Xiaojuan Xu, Yinghong Huang
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cervical cancer Daidzin Apoptosis HeLa cell Caspases Inflammation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8af660c6cc3d48069455cb51d73e348d
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spelling	oai:doaj.org-article:8af660c6cc3d48069455cb51d73e348d2021-11-20T04:56:48ZDaidzin inhibits growth and induces apoptosis through the JAK2/STAT3 in human cervical cancer HeLa cells1319-562X10.1016/j.sjbs.2021.08.011https://doaj.org/article/8af660c6cc3d48069455cb51d73e348d2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319562X21006847https://doaj.org/toc/1319-562XDaidzin, 4′, 7-dihydroxyisoflavone is an isoflavonic phytoestrogen present in leguminous plants. Traditional Chinese medicine utilizes daidzin to treat various diseases such diarrhea, fever, hepatitis, cardiac problems etc. In current study we examined the anticancer activity of daidzin against human cervical cancer in vitro. HeLa, human cervical cancer cell line was purchased from ATCC and the cells were cultured with DMEM medium. The cytotoxic effect of daidzin against HeLa cell line was analyzed with MTT assay. The IC-50 value was obtained at 20 µM hence the cells were treated with 20 µM of daidzin for further analysis. ROS generation was assessed with DCFH-DA staining and the induction of apoptosis was examined with Rhoadmine-123 staining. Acridine orange and ethidium bromide staining was done to examine the apoptotic and viable cells. Further the matrigel cell adhesion assay was done to analyze the inhibitory property of daidzin against cancer cell adhesion. Apoptotic induction of daidzin was examined by estimating the levels of Caspase 8 & 9 using ELISA technique. Inflammatory and cell proliferation signaling proteins were analyzed with qPCR analysis to confirm the anticancer activity of daidzin against human cervical cancer HeLa cell line. Daidzin significantly generated ROS and altered the mitochondrial membrane permeability in HeLa cell line. The results of AO/EtBr staining prove daidzin induced apoptosis in HeLa cell line and it also inhibited the cell adhesion property of HeLa which is reported in our matrigel cell adhesion assay. It also increased the caspases 8 & 9 which are key regulators of apoptosis. Daidzin significantly decreased the expression of inflammatory gene and cell proliferating signaling molecule. To, conclude our results confirm daidzin effectively decreased inflammation and induced apoptosis in human cervical cancer HeLa cell line.Zhilin YaoXiaojuan XuYinghong HuangElsevierarticleCervical cancerDaidzinApoptosisHeLa cellCaspasesInflammationBiology (General)QH301-705.5ENSaudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 7077-7081 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cervical cancer Daidzin Apoptosis HeLa cell Caspases Inflammation Biology (General) QH301-705.5
spellingShingle	Cervical cancer Daidzin Apoptosis HeLa cell Caspases Inflammation Biology (General) QH301-705.5 Zhilin Yao Xiaojuan Xu Yinghong Huang Daidzin inhibits growth and induces apoptosis through the JAK2/STAT3 in human cervical cancer HeLa cells
description	Daidzin, 4′, 7-dihydroxyisoflavone is an isoflavonic phytoestrogen present in leguminous plants. Traditional Chinese medicine utilizes daidzin to treat various diseases such diarrhea, fever, hepatitis, cardiac problems etc. In current study we examined the anticancer activity of daidzin against human cervical cancer in vitro. HeLa, human cervical cancer cell line was purchased from ATCC and the cells were cultured with DMEM medium. The cytotoxic effect of daidzin against HeLa cell line was analyzed with MTT assay. The IC-50 value was obtained at 20 µM hence the cells were treated with 20 µM of daidzin for further analysis. ROS generation was assessed with DCFH-DA staining and the induction of apoptosis was examined with Rhoadmine-123 staining. Acridine orange and ethidium bromide staining was done to examine the apoptotic and viable cells. Further the matrigel cell adhesion assay was done to analyze the inhibitory property of daidzin against cancer cell adhesion. Apoptotic induction of daidzin was examined by estimating the levels of Caspase 8 & 9 using ELISA technique. Inflammatory and cell proliferation signaling proteins were analyzed with qPCR analysis to confirm the anticancer activity of daidzin against human cervical cancer HeLa cell line. Daidzin significantly generated ROS and altered the mitochondrial membrane permeability in HeLa cell line. The results of AO/EtBr staining prove daidzin induced apoptosis in HeLa cell line and it also inhibited the cell adhesion property of HeLa which is reported in our matrigel cell adhesion assay. It also increased the caspases 8 & 9 which are key regulators of apoptosis. Daidzin significantly decreased the expression of inflammatory gene and cell proliferating signaling molecule. To, conclude our results confirm daidzin effectively decreased inflammation and induced apoptosis in human cervical cancer HeLa cell line.
format	article
author	Zhilin Yao Xiaojuan Xu Yinghong Huang
author_facet	Zhilin Yao Xiaojuan Xu Yinghong Huang
author_sort	Zhilin Yao
title	Daidzin inhibits growth and induces apoptosis through the JAK2/STAT3 in human cervical cancer HeLa cells
title_short	Daidzin inhibits growth and induces apoptosis through the JAK2/STAT3 in human cervical cancer HeLa cells
title_full	Daidzin inhibits growth and induces apoptosis through the JAK2/STAT3 in human cervical cancer HeLa cells
title_fullStr	Daidzin inhibits growth and induces apoptosis through the JAK2/STAT3 in human cervical cancer HeLa cells
title_full_unstemmed	Daidzin inhibits growth and induces apoptosis through the JAK2/STAT3 in human cervical cancer HeLa cells
title_sort	daidzin inhibits growth and induces apoptosis through the jak2/stat3 in human cervical cancer hela cells
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/8af660c6cc3d48069455cb51d73e348d
work_keys_str_mv	AT zhilinyao daidzininhibitsgrowthandinducesapoptosisthroughthejak2stat3inhumancervicalcancerhelacells AT xiaojuanxu daidzininhibitsgrowthandinducesapoptosisthroughthejak2stat3inhumancervicalcancerhelacells AT yinghonghuang daidzininhibitsgrowthandinducesapoptosisthroughthejak2stat3inhumancervicalcancerhelacells
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Daidzin inhibits growth and induces apoptosis through the JAK2/STAT3 in human cervical cancer HeLa cells

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