High-fidelity CRISPR/Cas9- based gene-specific hydroxymethylation rescues gene expression and attenuates renal fibrosis

Suppression of gene expression due to aberrant promoter methylation contributes to organ fibrosis. Here, the authors couple a deactivated Cas9 to the TET3 catalytic domain to induce expression of four antifibrotic genes, and show that lentiviral-mediated delivery is effective in reducing kidney fibr...

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Autores principales:	Xingbo Xu, Xiaoying Tan, Björn Tampe, Tim Wilhelmi, Melanie S. Hulshoff, Shoji Saito, Tobias Moser, Raghu Kalluri, Gerd Hasenfuss, Elisabeth M. Zeisberg, Michael Zeisberg
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/8afce270d13543dc99f4fba3b97f9f22
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spelling	oai:doaj.org-article:8afce270d13543dc99f4fba3b97f9f222021-12-02T15:34:29ZHigh-fidelity CRISPR/Cas9- based gene-specific hydroxymethylation rescues gene expression and attenuates renal fibrosis10.1038/s41467-018-05766-52041-1723https://doaj.org/article/8afce270d13543dc99f4fba3b97f9f222018-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05766-5https://doaj.org/toc/2041-1723Suppression of gene expression due to aberrant promoter methylation contributes to organ fibrosis. Here, the authors couple a deactivated Cas9 to the TET3 catalytic domain to induce expression of four antifibrotic genes, and show that lentiviral-mediated delivery is effective in reducing kidney fibrosis in mouse models.Xingbo XuXiaoying TanBjörn TampeTim WilhelmiMelanie S. HulshoffShoji SaitoTobias MoserRaghu KalluriGerd HasenfussElisabeth M. ZeisbergMichael ZeisbergNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-15 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Xingbo Xu Xiaoying Tan Björn Tampe Tim Wilhelmi Melanie S. Hulshoff Shoji Saito Tobias Moser Raghu Kalluri Gerd Hasenfuss Elisabeth M. Zeisberg Michael Zeisberg High-fidelity CRISPR/Cas9- based gene-specific hydroxymethylation rescues gene expression and attenuates renal fibrosis
description	Suppression of gene expression due to aberrant promoter methylation contributes to organ fibrosis. Here, the authors couple a deactivated Cas9 to the TET3 catalytic domain to induce expression of four antifibrotic genes, and show that lentiviral-mediated delivery is effective in reducing kidney fibrosis in mouse models.
format	article
author	Xingbo Xu Xiaoying Tan Björn Tampe Tim Wilhelmi Melanie S. Hulshoff Shoji Saito Tobias Moser Raghu Kalluri Gerd Hasenfuss Elisabeth M. Zeisberg Michael Zeisberg
author_facet	Xingbo Xu Xiaoying Tan Björn Tampe Tim Wilhelmi Melanie S. Hulshoff Shoji Saito Tobias Moser Raghu Kalluri Gerd Hasenfuss Elisabeth M. Zeisberg Michael Zeisberg
author_sort	Xingbo Xu
title	High-fidelity CRISPR/Cas9- based gene-specific hydroxymethylation rescues gene expression and attenuates renal fibrosis
title_short	High-fidelity CRISPR/Cas9- based gene-specific hydroxymethylation rescues gene expression and attenuates renal fibrosis
title_full	High-fidelity CRISPR/Cas9- based gene-specific hydroxymethylation rescues gene expression and attenuates renal fibrosis
title_fullStr	High-fidelity CRISPR/Cas9- based gene-specific hydroxymethylation rescues gene expression and attenuates renal fibrosis
title_full_unstemmed	High-fidelity CRISPR/Cas9- based gene-specific hydroxymethylation rescues gene expression and attenuates renal fibrosis
title_sort	high-fidelity crispr/cas9- based gene-specific hydroxymethylation rescues gene expression and attenuates renal fibrosis
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/8afce270d13543dc99f4fba3b97f9f22
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High-fidelity CRISPR/Cas9- based gene-specific hydroxymethylation rescues gene expression and attenuates renal fibrosis

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