Chromosomal integration of HHV-6A during non-productive viral infection

Abstract Human herpesvirus 6A (HHV-6A) and 6B (HHV-6B) are two different species of betaherpesviruses that integrate into sub-telomeric ends of human chromosomes, for which different prevalence rates of integration have been reported. It has been demonstrated that integrated viral genome is stable a...

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Autores principales:	Nitish Gulve, Celina Frank, Maximilian Klepsch, Bhupesh K. Prusty
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8afd52bd2a7447dfa6c0844cae6611e0
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spelling	oai:doaj.org-article:8afd52bd2a7447dfa6c0844cae6611e02021-12-02T11:41:01ZChromosomal integration of HHV-6A during non-productive viral infection10.1038/s41598-017-00658-y2045-2322https://doaj.org/article/8afd52bd2a7447dfa6c0844cae6611e02017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00658-yhttps://doaj.org/toc/2045-2322Abstract Human herpesvirus 6A (HHV-6A) and 6B (HHV-6B) are two different species of betaherpesviruses that integrate into sub-telomeric ends of human chromosomes, for which different prevalence rates of integration have been reported. It has been demonstrated that integrated viral genome is stable and is fully retained. However, study of chromosomally integrated viral genome in individuals carrying inherited HHV-6 (iciHHV-6) showed unexpected number of viral DR copies. Hence, we created an in vitro infection model and studied retention of full or partial viral genome over a period of time. We observed an exceptional event where cells retained viral direct repeats (DRs) alone in the absence of the full viral genome. Finally, we found evidence for non-telomeric integration of HHV-6A DR in both cultured cells and in an iciHHV-6 individual. Our results shed light on several novel features of HHV-6A chromosomal integration and provide valuable information for future screening techniques.Nitish GulveCelina FrankMaximilian KlepschBhupesh K. PrustyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nitish Gulve Celina Frank Maximilian Klepsch Bhupesh K. Prusty Chromosomal integration of HHV-6A during non-productive viral infection
description	Abstract Human herpesvirus 6A (HHV-6A) and 6B (HHV-6B) are two different species of betaherpesviruses that integrate into sub-telomeric ends of human chromosomes, for which different prevalence rates of integration have been reported. It has been demonstrated that integrated viral genome is stable and is fully retained. However, study of chromosomally integrated viral genome in individuals carrying inherited HHV-6 (iciHHV-6) showed unexpected number of viral DR copies. Hence, we created an in vitro infection model and studied retention of full or partial viral genome over a period of time. We observed an exceptional event where cells retained viral direct repeats (DRs) alone in the absence of the full viral genome. Finally, we found evidence for non-telomeric integration of HHV-6A DR in both cultured cells and in an iciHHV-6 individual. Our results shed light on several novel features of HHV-6A chromosomal integration and provide valuable information for future screening techniques.
format	article
author	Nitish Gulve Celina Frank Maximilian Klepsch Bhupesh K. Prusty
author_facet	Nitish Gulve Celina Frank Maximilian Klepsch Bhupesh K. Prusty
author_sort	Nitish Gulve
title	Chromosomal integration of HHV-6A during non-productive viral infection
title_short	Chromosomal integration of HHV-6A during non-productive viral infection
title_full	Chromosomal integration of HHV-6A during non-productive viral infection
title_fullStr	Chromosomal integration of HHV-6A during non-productive viral infection
title_full_unstemmed	Chromosomal integration of HHV-6A during non-productive viral infection
title_sort	chromosomal integration of hhv-6a during non-productive viral infection
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8afd52bd2a7447dfa6c0844cae6611e0
work_keys_str_mv	AT nitishgulve chromosomalintegrationofhhv6aduringnonproductiveviralinfection AT celinafrank chromosomalintegrationofhhv6aduringnonproductiveviralinfection AT maximilianklepsch chromosomalintegrationofhhv6aduringnonproductiveviralinfection AT bhupeshkprusty chromosomalintegrationofhhv6aduringnonproductiveviralinfection
_version_	1718395471772254208

Chromosomal integration of HHV-6A during non-productive viral infection

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