SCF ensures meiotic chromosome segregation through a resolution of meiotic recombination intermediates.

The SCF (Skp1-Cul1-F-box) complex contributes to a variety of cellular events including meiotic cell cycle control, but its function during meiosis is not understood well. Here we describe a novel function of SCF/Skp1 in meiotic recombination and subsequent chromosome segregation. The skp1 temperatu...

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Autores principales:	Shin-ya Okamoto, Masamitsu Sato, Takashi Toda, Masayuki Yamamoto
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8aff3f567a37487cb24fc69e4c1c836e
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spelling	oai:doaj.org-article:8aff3f567a37487cb24fc69e4c1c836e2021-11-18T07:29:35ZSCF ensures meiotic chromosome segregation through a resolution of meiotic recombination intermediates.1932-620310.1371/journal.pone.0030622https://doaj.org/article/8aff3f567a37487cb24fc69e4c1c836e2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22292001/?tool=EBIhttps://doaj.org/toc/1932-6203The SCF (Skp1-Cul1-F-box) complex contributes to a variety of cellular events including meiotic cell cycle control, but its function during meiosis is not understood well. Here we describe a novel function of SCF/Skp1 in meiotic recombination and subsequent chromosome segregation. The skp1 temperature-sensitive mutant exhibited abnormal distribution of spindle microtubules in meiosis II, which turned out to originate from abnormal bending of the spindle in meiosis I. Bent spindles were reported in mitosis of this mutant, but it remained unknown how SCF could affect spindle morphology. We found that the meiotic bent spindle in skp1 cells was due to a hypertension generated by chromosome entanglement. The spindle bending was suppressed by inhibiting double strand break (DSB) formation, indicating that the entanglement was generated by the meiotic recombination machinery. Consistently, Rhp51/Rad51-Rad22/Rad52 foci persisted until meiosis I in skp1 cells, proving accumulation of recombination intermediates. Intriguingly bent spindles were also observed in the mutant of Fbh1, an F-box protein containing the DNA helicase domain, which is involved in meiotic recombination. Genetic evidence suggested its cooperation with SCF/Skp1. Thus, SCF/Skp1 together with Fbh1 is likely to function in the resolution of meiotic recombination intermediates, thereby ensuring proper chromosome segregation.Shin-ya OkamotoMasamitsu SatoTakashi TodaMasayuki YamamotoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e30622 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shin-ya Okamoto Masamitsu Sato Takashi Toda Masayuki Yamamoto SCF ensures meiotic chromosome segregation through a resolution of meiotic recombination intermediates.
description	The SCF (Skp1-Cul1-F-box) complex contributes to a variety of cellular events including meiotic cell cycle control, but its function during meiosis is not understood well. Here we describe a novel function of SCF/Skp1 in meiotic recombination and subsequent chromosome segregation. The skp1 temperature-sensitive mutant exhibited abnormal distribution of spindle microtubules in meiosis II, which turned out to originate from abnormal bending of the spindle in meiosis I. Bent spindles were reported in mitosis of this mutant, but it remained unknown how SCF could affect spindle morphology. We found that the meiotic bent spindle in skp1 cells was due to a hypertension generated by chromosome entanglement. The spindle bending was suppressed by inhibiting double strand break (DSB) formation, indicating that the entanglement was generated by the meiotic recombination machinery. Consistently, Rhp51/Rad51-Rad22/Rad52 foci persisted until meiosis I in skp1 cells, proving accumulation of recombination intermediates. Intriguingly bent spindles were also observed in the mutant of Fbh1, an F-box protein containing the DNA helicase domain, which is involved in meiotic recombination. Genetic evidence suggested its cooperation with SCF/Skp1. Thus, SCF/Skp1 together with Fbh1 is likely to function in the resolution of meiotic recombination intermediates, thereby ensuring proper chromosome segregation.
format	article
author	Shin-ya Okamoto Masamitsu Sato Takashi Toda Masayuki Yamamoto
author_facet	Shin-ya Okamoto Masamitsu Sato Takashi Toda Masayuki Yamamoto
author_sort	Shin-ya Okamoto
title	SCF ensures meiotic chromosome segregation through a resolution of meiotic recombination intermediates.
title_short	SCF ensures meiotic chromosome segregation through a resolution of meiotic recombination intermediates.
title_full	SCF ensures meiotic chromosome segregation through a resolution of meiotic recombination intermediates.
title_fullStr	SCF ensures meiotic chromosome segregation through a resolution of meiotic recombination intermediates.
title_full_unstemmed	SCF ensures meiotic chromosome segregation through a resolution of meiotic recombination intermediates.
title_sort	scf ensures meiotic chromosome segregation through a resolution of meiotic recombination intermediates.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/8aff3f567a37487cb24fc69e4c1c836e
work_keys_str_mv	AT shinyaokamoto scfensuresmeioticchromosomesegregationthrougharesolutionofmeioticrecombinationintermediates AT masamitsusato scfensuresmeioticchromosomesegregationthrougharesolutionofmeioticrecombinationintermediates AT takashitoda scfensuresmeioticchromosomesegregationthrougharesolutionofmeioticrecombinationintermediates AT masayukiyamamoto scfensuresmeioticchromosomesegregationthrougharesolutionofmeioticrecombinationintermediates
_version_	1718423357998759936

SCF ensures meiotic chromosome segregation through a resolution of meiotic recombination intermediates.

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