A haplotype in CFH family genes confers high risk of rare glomerular nephropathies
Abstract Despite distinct renal lesions, a series of rare glomerular nephropathies are reportedly mediated by complement overactivation. Genetic variations in complement genes contribute to disease risk, but the relationship of genotype to phenotype has not been straightforward. Here, we screened 11...
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Nature Portfolio
2017
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oai:doaj.org-article:8b01a17b909c4a4fb5cba79f402d8a1c2021-12-02T16:08:19ZA haplotype in CFH family genes confers high risk of rare glomerular nephropathies10.1038/s41598-017-05173-82045-2322https://doaj.org/article/8b01a17b909c4a4fb5cba79f402d8a1c2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05173-8https://doaj.org/toc/2045-2322Abstract Despite distinct renal lesions, a series of rare glomerular nephropathies are reportedly mediated by complement overactivation. Genetic variations in complement genes contribute to disease risk, but the relationship of genotype to phenotype has not been straightforward. Here, we screened 11 complement genes from 91 patients with atypical hemolytic uremic syndrome (aHUS), C3 glomerulopathy (C3G) and membranoproliferative glomerulonephritis type I (MPGN I), and identified the concomitant presence of three missense variations located within the human complement Factor H (CFH) gene cluster. The three variations, rs55807605, rs61737525 and rs57960694, have strong linkage disequilibrium; subsequent haplotype analysis indicated that ATA increased the susceptibility of these renal diseases. In silico analysis, the CFHR3 rs61737525-T risk allele altered the physical and structural properties and generated a reduction in binding affinity of the CFHR3/C3b complex. Surface plasmon resonance (SPR) binding analysis further demonstrated the substitution induced a decrease of two orders of magnitude in C3b-binding properties, with a declined cofactor activity in fluid phase. These data suggest that the haplotype carrying the causative allele behaves as a partial C3 convertase deficiency, predisposing individuals to diverse pathologic lesions underlying complement overactivation. Such genotype-phenotype discrepancies allow better understanding about these nephropathies mediated by genetic complement disorders.Yin DingWeiwei ZhaoTao ZhangHao QiangJianping LuXin SuShuzhen WenFeng XuMingchao ZhangHaitao ZhangCaihong ZengZhihong LiuHuimei ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) |
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Medicine R Science Q Yin Ding Weiwei Zhao Tao Zhang Hao Qiang Jianping Lu Xin Su Shuzhen Wen Feng Xu Mingchao Zhang Haitao Zhang Caihong Zeng Zhihong Liu Huimei Chen A haplotype in CFH family genes confers high risk of rare glomerular nephropathies |
| description |
Abstract Despite distinct renal lesions, a series of rare glomerular nephropathies are reportedly mediated by complement overactivation. Genetic variations in complement genes contribute to disease risk, but the relationship of genotype to phenotype has not been straightforward. Here, we screened 11 complement genes from 91 patients with atypical hemolytic uremic syndrome (aHUS), C3 glomerulopathy (C3G) and membranoproliferative glomerulonephritis type I (MPGN I), and identified the concomitant presence of three missense variations located within the human complement Factor H (CFH) gene cluster. The three variations, rs55807605, rs61737525 and rs57960694, have strong linkage disequilibrium; subsequent haplotype analysis indicated that ATA increased the susceptibility of these renal diseases. In silico analysis, the CFHR3 rs61737525-T risk allele altered the physical and structural properties and generated a reduction in binding affinity of the CFHR3/C3b complex. Surface plasmon resonance (SPR) binding analysis further demonstrated the substitution induced a decrease of two orders of magnitude in C3b-binding properties, with a declined cofactor activity in fluid phase. These data suggest that the haplotype carrying the causative allele behaves as a partial C3 convertase deficiency, predisposing individuals to diverse pathologic lesions underlying complement overactivation. Such genotype-phenotype discrepancies allow better understanding about these nephropathies mediated by genetic complement disorders. |
| format |
article |
| author |
Yin Ding Weiwei Zhao Tao Zhang Hao Qiang Jianping Lu Xin Su Shuzhen Wen Feng Xu Mingchao Zhang Haitao Zhang Caihong Zeng Zhihong Liu Huimei Chen |
| author_facet |
Yin Ding Weiwei Zhao Tao Zhang Hao Qiang Jianping Lu Xin Su Shuzhen Wen Feng Xu Mingchao Zhang Haitao Zhang Caihong Zeng Zhihong Liu Huimei Chen |
| author_sort |
Yin Ding |
| title |
A haplotype in CFH family genes confers high risk of rare glomerular nephropathies |
| title_short |
A haplotype in CFH family genes confers high risk of rare glomerular nephropathies |
| title_full |
A haplotype in CFH family genes confers high risk of rare glomerular nephropathies |
| title_fullStr |
A haplotype in CFH family genes confers high risk of rare glomerular nephropathies |
| title_full_unstemmed |
A haplotype in CFH family genes confers high risk of rare glomerular nephropathies |
| title_sort |
haplotype in cfh family genes confers high risk of rare glomerular nephropathies |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/8b01a17b909c4a4fb5cba79f402d8a1c |
| work_keys_str_mv |
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