FePt nanoparticles as a potential X-ray activated chemotherapy agent for HeLa cells

Yanhong Zheng,1 Yunlan Tang,2 Zhirong Bao,1 Hui Wang,1 Feng Ren,1 Mingxiong Guo,2 Hong Quan,1 Changzhong Jiang11Key Laboratory of Artificial Micro- and Nano-structures of the Ministry of Education and Center for Electronic Microscopy and Department of Physics, Wuhan University; 2College of Life Scie...

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Autores principales:	Zheng Y, Tang Y, Bao Z, Wang H, Ren F, Guo M, Quan H, Jiang C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8b05b3252bbf48a5a7f12b689aed5537
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spelling	oai:doaj.org-article:8b05b3252bbf48a5a7f12b689aed55372021-12-02T07:46:16ZFePt nanoparticles as a potential X-ray activated chemotherapy agent for HeLa cells1178-2013https://doaj.org/article/8b05b3252bbf48a5a7f12b689aed55372015-10-01T00:00:00Zhttps://www.dovepress.com/fept-nanoparticles-as-a-potential-x-ray-activated-chemotherapy-agent-f-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yanhong Zheng,1 Yunlan Tang,2 Zhirong Bao,1 Hui Wang,1 Feng Ren,1 Mingxiong Guo,2 Hong Quan,1 Changzhong Jiang11Key Laboratory of Artificial Micro- and Nano-structures of the Ministry of Education and Center for Electronic Microscopy and Department of Physics, Wuhan University; 2College of Life Sciences, Wuhan University, Wuhan, People’s Republic of ChinaAbstract: Nanomaterials have an advantage in “personalized” therapy, which is the ultimate goal of tumor treatment. In order to investigate the potential ability of FePt nanoparticles (NPs) in the diagnosis and chemoradiotherapy treatment of malignant tumors, superparamagnetic, monodispersed FePt (~3 nm) alloy NPs were synthesized, using cysteamine as a capping agent. The NPs were characterized by means of X-ray diffraction; transmission electron microscopy, Physical Property Measurement System, and Fourier transform infrared spectroscopy. The cytotoxicity of FePt NPs on Vero cells was assessed using an MTT assay, and tumor cell proliferation inhibited by individual FePt NPs and FePt NPs combined with X-ray beams were also collected using MTT assays; HeLa human cancer cell lines were used as in vitro models. Further confirmation of the combined effect of FePt NPs and X-rays was verified using HeLa cells, after which, the cellular uptake of FePt NPs was captured by transmission electron microscopy. The results indicated that the growth of HeLa cells was significantly inhibited by FePt NPs in a concentration-dependent manner, and the growth was significantly more inhibited by FePt NPs combined with a series of X-ray beam doses; the individual NPs did not display any remarkable cytotoxicity on Vero cells at a concentration <250 µg/mL. Meanwhile, the FePt NPs showed negative/positive contrast enhancement for MRI/CT molecule imaging at the end of the study. Therefore, the combined results implied that FePt NPs might potentially serve as a promising nanoprobe for the integration of tumor diagnosis and chemoradiotherapy. Keywords: superparamagnetism, MRI/CT, chemoradiotherapy, intelligent nanoprobeZheng YTang YBao ZWang HRen FGuo MQuan HJiang CDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 6435-6444 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zheng Y Tang Y Bao Z Wang H Ren F Guo M Quan H Jiang C FePt nanoparticles as a potential X-ray activated chemotherapy agent for HeLa cells
description	Yanhong Zheng,1 Yunlan Tang,2 Zhirong Bao,1 Hui Wang,1 Feng Ren,1 Mingxiong Guo,2 Hong Quan,1 Changzhong Jiang11Key Laboratory of Artificial Micro- and Nano-structures of the Ministry of Education and Center for Electronic Microscopy and Department of Physics, Wuhan University; 2College of Life Sciences, Wuhan University, Wuhan, People’s Republic of ChinaAbstract: Nanomaterials have an advantage in “personalized” therapy, which is the ultimate goal of tumor treatment. In order to investigate the potential ability of FePt nanoparticles (NPs) in the diagnosis and chemoradiotherapy treatment of malignant tumors, superparamagnetic, monodispersed FePt (~3 nm) alloy NPs were synthesized, using cysteamine as a capping agent. The NPs were characterized by means of X-ray diffraction; transmission electron microscopy, Physical Property Measurement System, and Fourier transform infrared spectroscopy. The cytotoxicity of FePt NPs on Vero cells was assessed using an MTT assay, and tumor cell proliferation inhibited by individual FePt NPs and FePt NPs combined with X-ray beams were also collected using MTT assays; HeLa human cancer cell lines were used as in vitro models. Further confirmation of the combined effect of FePt NPs and X-rays was verified using HeLa cells, after which, the cellular uptake of FePt NPs was captured by transmission electron microscopy. The results indicated that the growth of HeLa cells was significantly inhibited by FePt NPs in a concentration-dependent manner, and the growth was significantly more inhibited by FePt NPs combined with a series of X-ray beam doses; the individual NPs did not display any remarkable cytotoxicity on Vero cells at a concentration <250 µg/mL. Meanwhile, the FePt NPs showed negative/positive contrast enhancement for MRI/CT molecule imaging at the end of the study. Therefore, the combined results implied that FePt NPs might potentially serve as a promising nanoprobe for the integration of tumor diagnosis and chemoradiotherapy. Keywords: superparamagnetism, MRI/CT, chemoradiotherapy, intelligent nanoprobe
format	article
author	Zheng Y Tang Y Bao Z Wang H Ren F Guo M Quan H Jiang C
author_facet	Zheng Y Tang Y Bao Z Wang H Ren F Guo M Quan H Jiang C
author_sort	Zheng Y
title	FePt nanoparticles as a potential X-ray activated chemotherapy agent for HeLa cells
title_short	FePt nanoparticles as a potential X-ray activated chemotherapy agent for HeLa cells
title_full	FePt nanoparticles as a potential X-ray activated chemotherapy agent for HeLa cells
title_fullStr	FePt nanoparticles as a potential X-ray activated chemotherapy agent for HeLa cells
title_full_unstemmed	FePt nanoparticles as a potential X-ray activated chemotherapy agent for HeLa cells
title_sort	fept nanoparticles as a potential x-ray activated chemotherapy agent for hela cells
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/8b05b3252bbf48a5a7f12b689aed5537
work_keys_str_mv	AT zhengy feptnanoparticlesasapotentialxrayactivatedchemotherapyagentforhelacells AT tangy feptnanoparticlesasapotentialxrayactivatedchemotherapyagentforhelacells AT baoz feptnanoparticlesasapotentialxrayactivatedchemotherapyagentforhelacells AT wangh feptnanoparticlesasapotentialxrayactivatedchemotherapyagentforhelacells AT renf feptnanoparticlesasapotentialxrayactivatedchemotherapyagentforhelacells AT guom feptnanoparticlesasapotentialxrayactivatedchemotherapyagentforhelacells AT quanh feptnanoparticlesasapotentialxrayactivatedchemotherapyagentforhelacells AT jiangc feptnanoparticlesasapotentialxrayactivatedchemotherapyagentforhelacells
_version_	1718399167740510208

FePt nanoparticles as a potential X-ray activated chemotherapy agent for HeLa cells

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