Transcription factors NF-YA2 and NF-YA10 regulate leaf growth via auxin signaling in Arabidopsis

Abstract In plants, leaf is crucial for photosynthesis and respiration. Leaf area and quantity are important for leaf vegetables to increase biomass. The process of leaf development involves coordinated regulation among small RNAs, transcription factors and hormones. Here, we found leaf size were re...

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Autores principales:	Min Zhang, Xiaolong Hu, Ming Zhu, Miaoyun Xu, Lei Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8b075cf5f220413a9d23d5ed81585774
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spelling	oai:doaj.org-article:8b075cf5f220413a9d23d5ed815857742021-12-02T12:32:59ZTranscription factors NF-YA2 and NF-YA10 regulate leaf growth via auxin signaling in Arabidopsis10.1038/s41598-017-01475-z2045-2322https://doaj.org/article/8b075cf5f220413a9d23d5ed815857742017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01475-zhttps://doaj.org/toc/2045-2322Abstract In plants, leaf is crucial for photosynthesis and respiration. Leaf area and quantity are important for leaf vegetables to increase biomass. The process of leaf development involves coordinated regulation among small RNAs, transcription factors and hormones. Here, we found leaf size were regulated by transcription factors NF-YA2 and NF-YA10 in Arabidopsis. NF-YA2 and NF-YA10 overexpression increased biomass accumulation through promoting leaf growth and cell expansion. NF-YA2 and NF-YA10 were expressed in SAM and leaf vasculature. Endogenous IAA content reduced by 20% and 24% in transgenic Arabidopsis plants overexpressing NF-YA2 and NF-YA10 compared to wild-type plants. Chromatin immunoprecipitation assays revealed that NF-YA2 and NF-YA10 bound directly to the cis-element CCAAT in the promoter of the YUC2, and decreased the expression of YUC2, a YUCCA family gene. The auxin transporter gene PIN1 and auxin response factor1 and 2 (ARF1 and ARF2) genes, transcriptional repressors, were downregulated. These findings showed leaf development was regulated by NF-YA2 and NF-YA10 through the auxin-signaling pathway and may provide a new insight into the genetic engineering of vegetables biomass and crop productivity.Min ZhangXiaolong HuMing ZhuMiaoyun XuLei WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Min Zhang Xiaolong Hu Ming Zhu Miaoyun Xu Lei Wang Transcription factors NF-YA2 and NF-YA10 regulate leaf growth via auxin signaling in Arabidopsis
description	Abstract In plants, leaf is crucial for photosynthesis and respiration. Leaf area and quantity are important for leaf vegetables to increase biomass. The process of leaf development involves coordinated regulation among small RNAs, transcription factors and hormones. Here, we found leaf size were regulated by transcription factors NF-YA2 and NF-YA10 in Arabidopsis. NF-YA2 and NF-YA10 overexpression increased biomass accumulation through promoting leaf growth and cell expansion. NF-YA2 and NF-YA10 were expressed in SAM and leaf vasculature. Endogenous IAA content reduced by 20% and 24% in transgenic Arabidopsis plants overexpressing NF-YA2 and NF-YA10 compared to wild-type plants. Chromatin immunoprecipitation assays revealed that NF-YA2 and NF-YA10 bound directly to the cis-element CCAAT in the promoter of the YUC2, and decreased the expression of YUC2, a YUCCA family gene. The auxin transporter gene PIN1 and auxin response factor1 and 2 (ARF1 and ARF2) genes, transcriptional repressors, were downregulated. These findings showed leaf development was regulated by NF-YA2 and NF-YA10 through the auxin-signaling pathway and may provide a new insight into the genetic engineering of vegetables biomass and crop productivity.
format	article
author	Min Zhang Xiaolong Hu Ming Zhu Miaoyun Xu Lei Wang
author_facet	Min Zhang Xiaolong Hu Ming Zhu Miaoyun Xu Lei Wang
author_sort	Min Zhang
title	Transcription factors NF-YA2 and NF-YA10 regulate leaf growth via auxin signaling in Arabidopsis
title_short	Transcription factors NF-YA2 and NF-YA10 regulate leaf growth via auxin signaling in Arabidopsis
title_full	Transcription factors NF-YA2 and NF-YA10 regulate leaf growth via auxin signaling in Arabidopsis
title_fullStr	Transcription factors NF-YA2 and NF-YA10 regulate leaf growth via auxin signaling in Arabidopsis
title_full_unstemmed	Transcription factors NF-YA2 and NF-YA10 regulate leaf growth via auxin signaling in Arabidopsis
title_sort	transcription factors nf-ya2 and nf-ya10 regulate leaf growth via auxin signaling in arabidopsis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8b075cf5f220413a9d23d5ed81585774
work_keys_str_mv	AT minzhang transcriptionfactorsnfya2andnfya10regulateleafgrowthviaauxinsignalinginarabidopsis AT xiaolonghu transcriptionfactorsnfya2andnfya10regulateleafgrowthviaauxinsignalinginarabidopsis AT mingzhu transcriptionfactorsnfya2andnfya10regulateleafgrowthviaauxinsignalinginarabidopsis AT miaoyunxu transcriptionfactorsnfya2andnfya10regulateleafgrowthviaauxinsignalinginarabidopsis AT leiwang transcriptionfactorsnfya2andnfya10regulateleafgrowthviaauxinsignalinginarabidopsis
_version_	1718393931700371456

Transcription factors NF-YA2 and NF-YA10 regulate leaf growth via auxin signaling in Arabidopsis

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